| Coltsfoot (Tussilago farfara L.) is a perennial plant of the Asteraceae family that has been used as an ancient folk remedy to treat coughs, bronchitis, and asthmatic disorders. Ripe seeds were collected from T. farfara plants growing in Pol-e Zangholeh, Iran, in this study. Samples were gathered by the Tarbiat Modares University (TMU) Department of Horticultural Sciences laboratory in late April 2016. The extracted seed essential oil constituents identified and quantified using GC/MS and GC, respectively. Phy,tol, n-Nonadecane, n-Tetradecane, and 4,4-Dimethyltetracyclo [5.2.1.02,6.03,5] decane, with amounts of 30.5%, 11.4%, 9.8%, and 6.2%, respectively, were the main components in the seeds oil of T. farfara. The germination variables were studied using a factorial experiment with 5 replicates × 50 seeds in a totally randomized design. Treatments include: cultivating non-pappus-bearing seeds in a Petri dish and placing them at 4°C (c1t1) and 25 °C (c1t2), Cultivating pappus-bearing seeds in a Petri dish and keeping them at 4 °C (c2t1) and 25 °C (c2t2) and cultivating of non-pappus-bearing seeds treated with sodium hypochlorite 1.5% for 20 minutes and keep them at 4 °C (c3t1) and 25 °C (c3t2). The interaction between culture type and temperature was significant (p≤0.01) for the germination percentage of seeds, germination rate, and seed vigor of T. farfara, according to analysis of variance. The rising seed germination rates of C3t2 and C3t1 were reported at 76.4% and 74.8%, respectively. The results revealed the closest positive correlation between germination rate, vigor index, and seed germination percentage (p≤0.001). |
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