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Nouroozi Kouh, T, Hoghooghi Rad, N, Navidpour, Sh, Shirali, S, Esmailnia, K. (1402). Molecular Identification of Cutaneous Leishmaniasis Vectors in Alborz Province, North of Iran. سامانه مدیریت نشریات علمی, 78(4), 1277-1284. doi: 10.32592/ARI.2023.78.4.1277
T Nouroozi Kouh; N Hoghooghi Rad; Sh Navidpour; S Shirali; K Esmailnia. "Molecular Identification of Cutaneous Leishmaniasis Vectors in Alborz Province, North of Iran". سامانه مدیریت نشریات علمی, 78, 4, 1402, 1277-1284. doi: 10.32592/ARI.2023.78.4.1277
Nouroozi Kouh, T, Hoghooghi Rad, N, Navidpour, Sh, Shirali, S, Esmailnia, K. (1402). 'Molecular Identification of Cutaneous Leishmaniasis Vectors in Alborz Province, North of Iran', سامانه مدیریت نشریات علمی, 78(4), pp. 1277-1284. doi: 10.32592/ARI.2023.78.4.1277
Nouroozi Kouh, T, Hoghooghi Rad, N, Navidpour, Sh, Shirali, S, Esmailnia, K. Molecular Identification of Cutaneous Leishmaniasis Vectors in Alborz Province, North of Iran. سامانه مدیریت نشریات علمی, 1402; 78(4): 1277-1284. doi: 10.32592/ARI.2023.78.4.1277

Molecular Identification of Cutaneous Leishmaniasis Vectors in Alborz Province, North of Iran

Archives of Razi Institute
مقاله 14، دوره 78، شماره 4، مهر و آبان 2023، صفحه 1277-1284    اصل مقاله (635.47 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2023.78.4.1277
نویسندگان
T Nouroozi Kouh1؛ N Hoghooghi Rad* 1؛ Sh Navidpour2؛ S Shirali1، 3؛ K Esmailnia2
1Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3Department of Biotechnology, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran
چکیده
Cutaneous leishmaniasis (CL) is a vector-borne disease widely distributed in tropical and subtropical areas of North and South America, Europe, Asia, and Africa. Considering the increasing number of CL cases in recent years and the fact that no study has been conducted to identify CL fauna and vectors in Alborz province, this study was carried out to identify sand flies and CL vectors in this region. Sand flies were collected from August to October 2021 from plain and mountainous indoor and outdoor areas of the region using sticky paper traps and were detected morphologically. DNA was extracted from the midguts of female sand flies. In this study, 1157 sand flies were collected and identified. The number of sand flies caught from indoor and outdoor places was 367 (31.72%) and 790 (68.28%), respectively. Overall, six species of flies were of the genus Phlebotomus (Raynal, 1937), including Phlebotomus papatasi (P. papatasi, 695 [60.07%]; Scopoli, 1786), P. kandelakii (13 [1.12%]; Shchurenkova, 1926), P. sergenti (232 [20.05%]; Parrot, 1917), P. major (14 [1.21%]; Annandale, 1910), P. caucasicus (4 [0.35%]; Marzinowsky, 1917), P. alexandri (18 [1.56%]; Alexandri Sinton, 1920), and four were of the genus Sergentomyia (Artemiev, 1978), including Sergentomyia tiberiadis (109 [9.42%]; Adler, Theodor & Lourie, 1930), Sergentomyia baghdadis (53 [4.58%]), Sergentomyia sintoni (14 [1.21%]; Sintoni Pringle, 1933), Sergentomyia clydei  (5 [0.43%]). P. papatasi spp. were dominant in indoor and outdoor places, with a prevalence of 695 (60.07%). The Leishmania major (L. major) gene was identified in five samples of P. papatasi spp. This suggests that P. papatasi is the potential vector spp. in the study area. Moreover, L. major was confirmed as the aetiological agent of CL cases in Alborz province. The identification of vectors and parasite spp. is very important for the treatment and operational planning of disease vectors.
کلیدواژه‌ها
kDNA؛ Leishmania major؛ Nested-PCR؛ Phlebotomus
عنوان مقاله [English]
تشخیص مولکولی ناقلین لیشمانیوز جلدی در استان البرز، شمال ایران
چکیده [English]
لیشمانیوز جلدی یک بیماری ناقل زاد است که به طور گسترده در مناطق گرمسیری و نیمه گرمسیری آمریکای شمالی و جنوبی، اروپا، آسیا و آفریقا پخش شده است. با توجه به روند رو به افزایش موارد لیشمانیوز جلدی در سال های اخیر و با توجه به اینکه تاکنون مطالعه ای در زمینه شناسایی ناقلین لیشمانیوز جلدی در استان البرز انجام نشده است، این مطالعه برای اولین بار به منظور شناسایی فون پشه های خاکی و ناقلین لیشمانیوز جلدی در منطقه انجام شد. پشه خاکی ها در طی ماه های مرداد، شهریور و مهر 1400 از اماکن داخلی و خارجی، در مناطق دشت و کوهستان با استفاده از تله های چسبان جمع آوری شدند و از نظر مورفولوژیکی شناسایی گردیدند. DNA از روده پشه خاکی های ماده استخراج شد. در این مطالعه 1157 پشه خاکی جمع آوری و شناسایی شد. تعداد پشه خاکی های صید شده از مکان های داخلی و خارجی به ترتیب 367(72/31%) و 790(28/68%) بودند. در مجموع، شش گونه از جنس Phlebotomus (Raynal, 1937) شامل (Scopoli, 1786) Phlebotomus papatasi 695(07/60%)، (Shchurenkova, 1926) Phlebotomus kandelakii 13(12/1%)، Phlebotomus sergenti (Parrot, 1917) 232(05/20%)، Phlebotomus major (Annandale, 1910) 14(21/1%)، Phlebotomus caucasicus (Marzinowsky,1917) 4(35/0%)، Phlebotomus alexanderi 18(56/1%)، و چهار گونه از جنس Sergentomyia (Artemiev, 1978) شامل Sergentomyia tiberiadis 109(42/9%)، Sergentomyia baghdadis 53(58/4%)، Sergentomyia sintoni 14(21/1%)، Sergentomyia clydei 5(43/0%)، بودند. گونه Phlebotomus papatasi با شیوع 07/60 درصد نمونه غالب در اماکن داخلی و خارجی بود. ژن لیشمانیا ماژور در پنج نمونه گونه Phlebotomus papatasi شناسایی شد. این مطالعه نشان داد و تایید کرد، که گونه Phlebotomus papatasi ناقل بالقوه و لیشمانیا ماژور عامل اتیولوژیک موارد لیشمانیوز جلدی در استان البرز است. شناسایی ناقلین و گونه های انگل برای درمان و برنامه ریزی عملیاتی کنترل ناقلین بیماری بسیار مهم است.
کلیدواژه‌ها [English]
لیشمانیا ماژور, kDNA, فلبوتوموس, Nested- PCR
مراجع
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  1. Shamsi L, Samaeinasab S. Prevalence of Cutaneous Leishmaniasis in the City of Sabzevar, 2013 to 2016. Int J Adv Boil Biomed Res. 2018;6(1):53-7.
  2. Yimam Ayene Y, Mohebali M, Hajjaran H, Akhoundi B, Shojaee S, Rahimi-Foroushani A, et al. A comparative study of nested-PCR and direct agglutination test (DAT) for the detection of Leishmania infantum infection in symptomatic and asymptomatic domestic dogs. BMC Res Notes. 2021;14(1):270.
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