اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,996
تعداد مقالات33,553
تعداد مشاهده مقاله44,161,103
تعداد دریافت فایل اصل مقاله20,534,253
Erdiansyah, Muhammad. (1402). Comparison of Lowenstein Jensen Media and Ogawa Media Usage for Viability Test of BCG Vaccine Pasteur P11732 and Russian (Moscow) – 384 sub-strains. سامانه مدیریت نشریات علمی, 79(1), 240-245. doi: 10.32592/ARI.2024.79.1.240
Muhammad Erdiansyah. "Comparison of Lowenstein Jensen Media and Ogawa Media Usage for Viability Test of BCG Vaccine Pasteur P11732 and Russian (Moscow) – 384 sub-strains". سامانه مدیریت نشریات علمی, 79, 1, 1402, 240-245. doi: 10.32592/ARI.2024.79.1.240
Erdiansyah, Muhammad. (1402). 'Comparison of Lowenstein Jensen Media and Ogawa Media Usage for Viability Test of BCG Vaccine Pasteur P11732 and Russian (Moscow) – 384 sub-strains', سامانه مدیریت نشریات علمی, 79(1), pp. 240-245. doi: 10.32592/ARI.2024.79.1.240
Erdiansyah, Muhammad. Comparison of Lowenstein Jensen Media and Ogawa Media Usage for Viability Test of BCG Vaccine Pasteur P11732 and Russian (Moscow) – 384 sub-strains. سامانه مدیریت نشریات علمی, 1402; 79(1): 240-245. doi: 10.32592/ARI.2024.79.1.240

Comparison of Lowenstein Jensen Media and Ogawa Media Usage for Viability Test of BCG Vaccine Pasteur P11732 and Russian (Moscow) – 384 sub-strains

Archives of Razi Institute
مقاله 26، دوره 79، شماره 1، فروردین و اردیبهشت 2024، صفحه 240-245    اصل مقاله (293.26 K)
نوع مقاله: Short Communication
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.1.240
نویسنده
Muhammad Erdiansyah*
National Quality Control Laboratory of Drug and Food (NQCLDF), Indonesian FDA, Central Jakarta, Indonesia
چکیده
BCG vaccines on the market have employed a Mycobacterium bovis sub-strains derived from the initial strain. To date, there has been no recommendation regarding the sub-strains with the highest effectiveness when administered to humans. Because it remains the standard for Tuberculosis treatment, the BCG vaccine's quality must be verified. One of the parameters for BCG vaccine quality control is the test for viability. The culture method has become the gold standard for viability testing with various testing media. This study aims to evaluate the performance of Lowenstein Jensen and Ogawa media for the viability test of Pasteur 1173P2 and Russian (Moscow) – 384 sub-strains of M. bovis in the BCG vaccine. The number of culturable particles of each sub-strain in the BCG vaccine was estimated and statistically evaluated using the t-test. The Colonies of the Pasteur 1173P2 has characteristics; tended to clump on both mediums with tiny, rough, and pale yellow/cream colors. While the colony character of the Russian (Moscow) – 384 generally has similar feature but did not cluster and had a smooth texture. In terms of growth rate, Lowenstein Jensen and Ogawa media performed similarly for Pasteur 1173P2 and Russian (Moscow) – 384 sub-strains. Maximum growth is reached by week 5. The culturable particles of Pasteur P1173P2 sub-strains did not differ between mediums. Whereas the growth of the Russian (Moscow) - 384 sub-strains was statistically better on Ogawa media. The results of this study reveal that the performance of the media used for determining the number of culturable particles is based on the sub-strains of M. bovis present in the BCG vaccine.
کلیدواژه‌ها
BCG Vaccine؛ Culture Media؛ Microbial Viability؛ Vaccine potency؛ Mycobacterium bovis
سایر فایل های مرتبط با مقاله
مراجع
References
1. Ritz N, Dutta B, Donath S, Casalaz D, Connell TG,
Tebruegge M, Robins-Browne R, Hanekom WA, Britton
WJ, Curtis N. The influence of bacille Calmette-Guérin
vaccine strain on the immune response against
tuberculosis: A randomized trial. Am J Respir Crit Care
Med. 2012; 185:213–222. doi: 10.1164/rccm.201104-
0714OC. PMID: 22071384.
2. Pascoe J, Hendon-Dunn CL, Birch CPD, Williams GA,
Chambers MA, Bacon J. Optimisation of mycobacterium
bovis BCG fermentation and storage survival.
Pharmaceutics. 2020; 12:1–14. doi:
10.3390/pharmaceutics12090900.
3. Palange P, Narang R, Kandi V. Evaluation of Culture
Media for Isolation of Mycobacterium Species from
Human Clinical Specimens. Cureus. 2016; 8:e757. doi:
10.7759/cureus.757.
4. Naveen G, Peerapur B v. Comparison of the
Lowenstein-Jensen medium, the middlebrook 7H10
medium and MB/BACT for the isolation of
Mycobacterium tuberculosis (MTB) from clinical
specimens. Journal of Clinical and Diagnostic Research.
2012; 6:1704–1709. doi: 10.7860/JCDR/2012/4603.2635.
PMID: 23373033.
5. Costa RR da, Silva SF da, Fochat RC, Macedo RL,
Pereira TV, Silva MR, Pinto CPG, Leite ICG.
Comparison between Ogawa-Kudoh and modified Petroff
techniques for mycobacteria cultivation in the diagnosis of
pulmonary tuberculosis. Einstein (Sao Paulo). 2018;
16:eAO4214. doi: 10.1590/S1679-45082018AO4214.
PMID: 29898027.
6. Franco-Sotomayor G, Rivera-Olivero IA, LeonBenitez M, Uruchima-Campoverde SE, Cardenas-Franco
G, Perdomo-Castro ME, Cardenas-Franco CS, OrtegaVivanco J, Abad-Ruiz AS, de Waard JH, et al. Fast,
Simple, and Cheap: the Kudoh-Ogawa Swab Method as
an Alternative to the Petroff-Lowenstein-Jensen Method
for Culturing of Mycobacterium tuberculosis. J Clin
Microbiol. 2020; 58:e01424-19. doi:
10.1128/JCM.01424-19.
7. Jensen KA. Bulletin of the International Union against
Tuberculosis. 1954; 24:78–112. PMID: 1756295.
8. Obayashi Y. Dried BCG Vaccine [Internet]. Geneva:
World Health Organization; 1955. Available from:
https://apps.who.int/iris/handle/10665/41663.
9. World Health Organization. In Vitro Assays of BCG
Products [Internet]. 1977. Available from:
https://apps.who.int/iris/handle/10665/99372.
10. Tanoue S, Mitarai S, Shishido H. Comparative study
on the use of solid media: Löwenstein-Jensen and Ogawa
in the determination of anti-tuberculosis drug
susceptibility. Tuberculosis. 2002; 82:63–67. doi:
10.1054/tube.2002.0323.

آمار
تعداد مشاهده مقاله: 11,248
تعداد دریافت فایل اصل مقاله: 497


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب