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تأثیر بیوچار و تنش خشکی بر برخی سازوکار‌های حفاظتی سیاه‌دانه (Nigella sativa L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 11، دوره 39، شماره 2، تیر 1402، صفحه 285-302 اصل مقاله (856.51 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2023.360517.3253 | ||
| نویسندگان | ||
| هاوری کیانی1؛ شیوا خالص رو* 2؛ علی مختصی بیدگلی3؛ زاهد شریفی4 | ||
| 1دانشجوی دکتری، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه کردستان، سنندج،ایران | ||
| 2استادیار، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه کردستان، سنندج، ایران | ||
| 3استادیار، گروه زراعت، دانشگاه تربیت مدرس، تهران، ایران | ||
| 4دانشیار، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه کردستان، سنندج، ایران | ||
| چکیده | ||
| سیاه‌دانه (Nigella sativa L.) از گیاهان دارویی ارزشمند است که در صنایع مختلف کاربرد وسیعی دارد. تجمع اسمولیت‌های سازگار از پاسخ‌های رایج گیاهان در رویارویی با تنش خشکی است. به‌منظور بررسی اثر رژیم‌های آبیاری و کاربرد بیوچار (حاصل از گرمادهی کود گاوی) بر گیاه دارویی سیاه‌دانه، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در گلخانه دانشکده کشاورزی دانشگاه کردستان در سال 1397 به اجرا درآمد. فاکتورهای آزمایش شامل تنش خشکی در سه سطح 100، 70 و 40 درصد ظرفیت زراعی و مصرف بیوچار در دو سطح 0 و 15 تن در هکتار بودند. نتـایج تجزیه واریانس داده‌ها نشان داد که اثرات متقابل سطوح تنش خشکی و کاربرد بیوچار روی پراکسید هیدروژن، مالون دی آلدئید، پراکسیداز، سوپراکسید دیسموتاز، پرولین، کربوهیدرات‌های محلول (محلول در آب و اتانول) و پتانسیل اسمزی معنی‌دار بود. افـزایش شدت تنش خشکی منجر به افزایش پراکسید هیدروژن، مالون دی آلدئید، پراکسیداز، سوپراکسید دیسموتاز، پرولین و کربوهیدرات‌های محلول (محلول در آب و اتانول) و منفی‌تر شدن پتانسیل اسمزی شد. کاربرد بیوچار سبب کاهش اثرات منفی تنش خشکی گردید، به‌طوری که میزان تولید پراکسید هیدروژن، مالون دی آلدئید، پراکسیداز، سوپراکسید دیسموتاز، پرولین و کربوهیدرات‌های محلول (در آب و الکل) نسبت به تیمار‌های فاقد بیوچار کمتر بود. به‌طور کلی نتایج تحقیق حاضر بیان‌کننده نقش مفید و مؤثر بیوچار در بهبود خصوصیات فیزیولوژیک و اسمولیت‌های محافظتی سیاه‌دانه در شرایط تنش خشکی بود. | ||
| کلیدواژه‌ها | ||
| آنزیم‌های آنتی‌اکسیدانی؛ اسمولیت‌های سازگار؛ پرولین؛ تنش اکسیداتیو؛ کمبود آب | ||
| عنوان مقاله [English] | ||
| Effects of biochar and drought stress on some protective mechanisms of Nigella sativa L. | ||
| نویسندگان [English] | ||
| H. Kiani1؛ Sh. Khalesro2؛ A. Mokhatssi-Bidgoli3؛ Z. Sharifi4 | ||
| 1Ph.D. student, Department of Plant Production and Genetics, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran | ||
| 2Assistant Professor, Department of Plant Production and Genetics, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran | ||
| 3Assistant Professor, Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 4Associate Professor, Department of Soil Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran | ||
| چکیده [English] | ||
| Nigella sativa L. is a valuable medicinal plant that is widely used in different industries. Accumulation of compatible osmolytes is one of the common responses of plants under drought stress. To investigate the effects of irrigation regimes and biochar (resulting from the heating of cattle manure) on N. sativa, a factorial experiment was conducted in a completely randomized design with three replications in the greenhouse of the Faculty of Agriculture at the University of Kurdistan in 2018. The experimental factors consisted of three drought stress levels (40, 70, and 100% of FC) and two biochar use levels (0 and 15 tons.ha-1). The ANOVA results showed that the interaction effects of drought stress and biochar were significant on hydrogen peroxide, malondialdehyde, superoxide dismutase, peroxidase, proline, soluble carbohydrates (water and ethanol soluble), and osmotic potential. Increasing the intensity of drought stress enhanced the amount of hydrogen peroxide, malondialdehyde, superoxide dismutase, peroxidase, proline, and soluble carbohydrates (water and ethanol soluble) and caused the osmotic potential to become more negative. Biochar application decreased the negative effects of drought stress so that hydrogen peroxide, malondialdehyde, superoxide dismutase, peroxidase, proline, and soluble carbohydrates (water and ethanol soluble) amounts were lower than the treatments without biochar. Overall, the present research results proved the useful and effective role of biochar in improving the physiological traits and protective osmolytes of N. sativa under drought stress. | ||
| کلیدواژه‌ها [English] | ||
| Antioxidant enzymes, compatible osmolytes, proline, oxidative stress, water deficiency | ||
| مراجع | ||
|
- Abbaspour, F., Asghari, H.R., Rezvani Moghaddam, P., Abbasdokht, H., Shabahang, J. and Baig Babaei, A., 2017. Effects of biochar application on yield and yield components of black seed (Nigella sativa L.) under low irrigation conditions. Iranian Journal of Medicinal and Aromatic Plants, 33: 837-852. - Abbas, T., Rizwan, M., Ali, S., Rehman, M.Z., Qayyum, M.F., Abbas, F., Hannan, F., Rinklebe, J. and Ok, Y.S., 2017. Effect of biochar on cadmium bioavailability and uptake in wheat (Triticum aestivum L.) grown in a soil with aged contamination. Ecotoxicology and Environmental Safety, 140: 37-47. - Ajithkumar, P. and Panneerselvam, R., 2013. Osmolyte accumulation, photosynthetic pigment and growth of Setaria italica under droght stress. Asian Pacific Journal, 2: 220-224. - Alexieva, V., Sergei, I., Mapelli, S. and Karanov, E., 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environment, 24: 1337-1344. - Bates, L.S., Waldern, R.P. and Tear, I.D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207. - Berek, A.K., Hue, N. and Ahmad, A., 2011. Beneficial use of biochar to correct soil acidity. The Food Provider, Available at www.ctahr.hawaii.edu/huen/nvh/biochar. - Bradford, M.M., 1979. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. - Chaitanya, K.V., Rasineni, G.K. and Reddy, A.R., 2009. Biochemical responses to drought stress in mulberry (Morus alba L.): evaluation of proline, glycine betaine and abscisic acid accumulation in five cultivars. Acta Physiology Plantarum, 31: - Darvizheh, H., Zahedi, M., Abbaszadeh, B., Razmjoo, J., 2019. Changes in some antioxidant enzymes and physiological indices of purple coneflower (Echinacea purpurea L.) in response to water deficit and foliar application of salicylic acid and spermine under field condition. Scientia Horticulturae, - Davey, M.W., Stals, E., Panis, B., Keulemans, J. and Swennen, R.L., 2005. High throughput of malondialdehyde in plant tissues. Analytical Biochemistry, 347: 201-207. - Dhindsa, R.S., Plumb-Dhindsa, P. and Thorpe, A., 1981. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32: 93-101. - Farhangi-Abriz, S. and Ghassemi-Golezani, K., 2022. The modified biochars influence nutrient and osmotic statuses and hormonal signaling of mint plants under fluoride and cadmium toxicities. Frontiers in Plant Scince, 13: 1064409. - Fallaha, S., Malekzadeha, S. and Pessarakli, M., 2018. Seed priming improves seedling emergence and reduces oxidative stress in Nigella sativa under soil moisture stress. Journal of Plant Nutrition, 41: 29-40. - Haj Seyed Hadi, M.R., Darzi, M.T. and Riazi, G.H., 2016. Black cumin (Nigella sativa L.) yield affected by irrigation and plant growth promoting bacteria. Journal of Medicinal Plants and By-products, 2: 125-133. - Hasanuzzaman, M., Alam, M.M., Rahman, A., Hasanuzzaman, M., Nahar, K. and Fujita, M., 2014. Exogenous proline and glycine betaine mediated upregulation of antioxidant defense and glyoxalase systems provides better protection against salt-induced oxidative stress in two rice (Oryza sativa L.) varieties. BioMed Research International, 2014: 757219 doi: 10.1155/2014/757219. - Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J. and Ahmad, A., 2012. Role of proline under changing environments. Plant Signaling and Behavior, 7(11): 1456-1466. - Hayati, A., Rahimi, M., Kelidari, A. and Hosseini, M., 2021. Effects of humic acid and iron nanochelate on osmolytes content of black cumin (Nigella sativa L.) under drought stress conditions. Iranian Journal of Medicinal and AromaticPlants Research, 37(5): 809-821. - Hedge, J.E. and Hofreiter, B.T., 1962. Carbohydrate chemistry: In R.L. Whistler, J.N. Be Miller, (Eds). Academic Press, New York, 1962p. - Hertig, E. and Tramblay, Y., 2017. Regional downscaling of Mediterranean droughts under past and future climatic conditions. Global and Planetary Change, 151: 36-48. - Hoerling, M., Eischeid, J., Perlwitz, J., Quan, X., Zhang, T. and Pegion, P., 2012. On the increased frequency of Mediterranean drought. Journal of Climate, 25: 2146-2161. - Kabiri, R., Nasibi, F. and Farahbakhsh, H., 2014. Effect of exogenous salicylic acid on some physiological parameters and alleviation of drought stress in Nigella sativa plant under hydroponic culture. Plant Protection Science, 50: 43-51. - Ghassemi-Golezani, K., Farhangi-Abriz, S., 2023. Biochar related treatments improved physiological performance, growth and productivity of Mentha crispa L. plants under fluoride and cadmium toxicities. Industrial Crops and Products, 194(1): 1-13. - Khaleghi Gazik, S., Saffari, V.R. and Daneshvar, S., 2021. Effect of different levels of biochar, hydrochar and salicylic acid on the growth of Calendula officinalis L. Iranian Journal of Horticultural Science, 53(3): 553-566. - KhasheiSiuki, A., Shahidi, A., Yaghoubzadeh, M. and Dastorani, M., 2019. Effect of Biochar Application and Irrigation Management on Yield and Yield Components Medicinal Plant (Trachyspermum ammi.). Journal of Irrigation and Drainage, 2: 319-328. - Khatun, S., Babar Ali, M., Hahn, E.J. and Paek, K.Y., 2008. Copper toxicity in Withania somnifera: Growth and antioxidant enzymes responses of in vitro grown plants. Environmental and Experimental Botany, 64: 279-285. - Kim, H.S., Kim, K.R., Yang, J.E., Ok, Y.S., Owens, G., Nehls, T., Wessolek, G. and Kim, K.H., 2016. Effect of biochar on reclaimed tidal land soil properties and maize (Zea mays L.) response. Chemosphere, 142: 153-159. - Kumar, A., Joseph, S., Tsechansky, L., Schreiter, I., Schuth, C., Taherysoosavi, S., Mitchell, D. and Graber, E., 2019. Mechanistic evaluation of biochar potential for plant growth promotion and alleviation of chromium-induced phytotoxicity in Ficus elastica. Chemosphere, 243: 1-23. - Laird, D.A., Fleming, P.D., Karlen, D.L., Wang, B. and Horton, R., 2010. Biochar impact on nutrient leaching from a Midwestern agricultural soil. Geoderma, 158: 436-442. - Lehmann, J. and Joseph, S. 2015. Biochar for Environmental Management Science, Technology and Implementation Second Edition. Scientific Research Publishing, Routledge, New York, 977p. - Liang, B., Lehmann, J., Solomon, D., Kinyangi, J., Grossman, J., O’Neill, B., Skjemstad, J.O., Theis, J., Luizao, F.J., Peterson, J. and Neves, E.G., 2006. Black carbon increases cation exchange capacity in soils. Soil Science Society of America Journal, - Lim, T.J., Spokas, K.A., Feyereisen, G. and Novak, J.M., 2016. Predicting the impact of biochar additions on soil hydraulic properties. Chemosphere, 142: 136-144. - Lotfi, R., Pessarakli, M., Gharavi-Kouchebagh, P. and Khoshvaghti, H., 2015. Physiological responses of Brassica napus to fulvic acid under water stress: chlorophyll a fluorescence and antioxidant enzyme activity. Crop Journal, 3: 434-439. - Mac-Adam, J.W., Nelson, C.J. and Sharp, R.E., 1992. Peroxidase activity in the leaf elongation zone of tall fescue. Plant Physiology, 99: 872-878. - Manavalan, p. and Nguyen, H.T., 2017. Drought Tolerance in Crops: Physiology to Genomics. Plant Stress Physiology, 2nd Edition Edited by Sergey Shabala. Boston, MA: CABI, 362p. - Matysik, J., Alia, A., Bhalu, B. and Mohanty, P., 2002. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science, 82: 525-532. - Naumann, G., Alfieri, L., Wyser, K., Mentaschi, L., Betts, R.A. and Carrao, H., 2018. Global changes in drought conditions under different levels of warming. Geophysical Research Letters, 45: 3285-3296. - Páscoa, P., Gouveia, C.M., Russo, A. and Trigo, R.M., 2012. The role of drought on wheat yield interannual variability in the Iberian Peninsula from 1929 to 2012. International Journal of Biometeorology, 61(3): 439-451. - Rahimzadeh, S. and Ghassemi-Golezani, K., 2022. Biochar-based nutritional nanocomposites altered nutrient uptake and vacuolar h+-pump activities of dill under salinity. Soil Science Plant Nutrient, 22: 3568-3581. - Rezaei-Chiyaneh, E., Seyyedi, S., Ebrahimian, M., Siavash, E., Moghaddam, S. and Damalas, C.A., 2018. Exogenous application of gamma-aminobutyric acid (GABA) alleviates the effect of water deficit stress in black cumin (Nigella sativa L.) Esmaeil. Industrial Crops and Products, 112: 741-748. - Safahani, A. and Noora, R., 2018. Effect of different levels of biochar on physiological traits of pumpkin under water shortage stress. Plant Environmental Physiology Journal, 13(49): 13-32. - Shahid, M., Pourrut, B., Dumat, C., Nadeem, M., Aslam, M. and Pinelli, E., 2014. Heavy-metal induced reactive oxygen species: phytotoxicity and physicochemical changes in plants. Reviews of Environmental Contamination and Toxicology, - Shulaev, V. and Oliver, D.J., 2006. Metabolic and proteomic markers for oxidative stress, new tools for reactive oxygen species research. Plant Physiology, 141: 367-372. - Sun, Y., Geng, Q., Du, Y., Yang, X. and Zhai, H., 2017. Induction of cyclic electron flow around photosystem I during heat stress in grape leaves. Plant Science, 256: 65-71. - Tiryaki, I., 2016. Drought stress and tolerance mechanisms in alfalfa (Medicago sativa L.). KSU Journal of Natural Science, 19: 296-305. - Unyayar, S., Kele, Y. and Cekic, F.O., 2005. The antioxidative response of two tomato species with different drought tolerances as a result of drought and cadmium stress combinations. Plant Soil Environment, 51(2): 57-64. - Verbruggen, N. and Hermans, C., 2008. Proline accumulation in plants: a review. Amino acids, 35: 753-759. - Zaki‚ R.N. and Radwan, T.E., 2011. Improving wheat grain yield and its quality under salinity conditions at a newly reclaimed soil by using different organic sources as soil or foliar applications. Journal of Applied Science Research, 7: 42-55. | ||
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