Mesgari Abbasi, Mehran, Khordadmehr, Monireh, Shanehbandi, Dariush, Jigari Asl, Farinaz, Teimuri-Mofrad, Reza, Tahmasebi, Shabnam, Asar, Mohammad Shahab, Eskandari, Fatemeh, Panahi, Yousef. (1402). Apoptosis Induction by New Coumarin Derivatives in a Mice Model of Breast Cancer. سامانه مدیریت نشریات علمی, 78(5), 1430-1439. doi: 10.32592/ARI.2023.78.5.1430
Mehran Mesgari Abbasi; Monireh Khordadmehr; Dariush Shanehbandi; Farinaz Jigari Asl; Reza Teimuri-Mofrad; Shabnam Tahmasebi; Mohammad Shahab Asar; Fatemeh Eskandari; Yousef Panahi. "Apoptosis Induction by New Coumarin Derivatives in a Mice Model of Breast Cancer". سامانه مدیریت نشریات علمی, 78, 5, 1402, 1430-1439. doi: 10.32592/ARI.2023.78.5.1430
Mesgari Abbasi, Mehran, Khordadmehr, Monireh, Shanehbandi, Dariush, Jigari Asl, Farinaz, Teimuri-Mofrad, Reza, Tahmasebi, Shabnam, Asar, Mohammad Shahab, Eskandari, Fatemeh, Panahi, Yousef. (1402). 'Apoptosis Induction by New Coumarin Derivatives in a Mice Model of Breast Cancer', سامانه مدیریت نشریات علمی, 78(5), pp. 1430-1439. doi: 10.32592/ARI.2023.78.5.1430
Mesgari Abbasi, Mehran, Khordadmehr, Monireh, Shanehbandi, Dariush, Jigari Asl, Farinaz, Teimuri-Mofrad, Reza, Tahmasebi, Shabnam, Asar, Mohammad Shahab, Eskandari, Fatemeh, Panahi, Yousef. Apoptosis Induction by New Coumarin Derivatives in a Mice Model of Breast Cancer. سامانه مدیریت نشریات علمی, 1402; 78(5): 1430-1439. doi: 10.32592/ARI.2023.78.5.1430
Apoptosis Induction by New Coumarin Derivatives in a Mice Model of Breast Cancer
1Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
3Immunology research center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
5Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
6Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
چکیده
In the last decades, numerous studies have focused on the search for new agents to suppress the growth of cancer cells. In this study, we investigated the effect of two novel synthetic coumarin derivatives, namely 2-amino-4-(4-(2-hydroxyethoxy)-3-methoxyphenyl)-5-oxo-4H,5H-pyrano[3,2-c]coumarin-3-carbonitrile and 2-amino-4-(4-hydroxyphenyl)-5-oxo-4H,5H-pyrano[3,2-c]coumarin-3-carbonitrile, on the induction of apoptosis in breast cancer in a mouse model. Breast cancer was induced in BALB/c mice, which were randomly divided into six groups and then underwent the experiment. The groups and treatments included A1: coumarin A with a low dose (10 µm), A2: coumarin A with a high dose (1 mM), B1: coumarin B with a low dose (10 µm), B2: coumarin B with a high dose (1 mM), D: doxorubicin, and C: cancer control/ treatment with normal saline. The samples underwent treatments for 5 weeks. Animals were euthanized, and tissue samples, including the lung, liver, and tumor mass, were collected for histopathological examination. In addition, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine some apoptotic markers, such as BCL-2, caspase-9, COX-2, and c-Myc. The qRT-PCR presented that both coumarin compounds could significantly alter the expression levels of BCL-2, caspase-9, COX-2, and c-Myc. Consistent with these results, histopathological observations showed a significant reduction in pathological lesions and severity of malignancy of the tumor mass, as well as a decrease in microscopic metastases in the lung and liver. This suggests that the present new coumarin compounds may induce apoptosis in breast cancer cells by altering some apoptosis-related genes that may play a chemotherapeutic role in breast cancer therapy in the future.
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