Masihipour, S, Sabiza, S, Rezaie, A, Ghaderi Ghahfarokhi, M. (1402). Histopathological Evaluation of Eggshell and DBM Combination on the Repair of Critical Size Experimental Calvarial Bone Defects in Rats. سامانه مدیریت نشریات علمی, 78(6), 1709-1718. doi: 10.32592/ARI.2023.78.6.1709
S Masihipour; S Sabiza; A Rezaie; M Ghaderi Ghahfarokhi. "Histopathological Evaluation of Eggshell and DBM Combination on the Repair of Critical Size Experimental Calvarial Bone Defects in Rats". سامانه مدیریت نشریات علمی, 78, 6, 1402, 1709-1718. doi: 10.32592/ARI.2023.78.6.1709
Masihipour, S, Sabiza, S, Rezaie, A, Ghaderi Ghahfarokhi, M. (1402). 'Histopathological Evaluation of Eggshell and DBM Combination on the Repair of Critical Size Experimental Calvarial Bone Defects in Rats', سامانه مدیریت نشریات علمی, 78(6), pp. 1709-1718. doi: 10.32592/ARI.2023.78.6.1709
Masihipour, S, Sabiza, S, Rezaie, A, Ghaderi Ghahfarokhi, M. Histopathological Evaluation of Eggshell and DBM Combination on the Repair of Critical Size Experimental Calvarial Bone Defects in Rats. سامانه مدیریت نشریات علمی, 1402; 78(6): 1709-1718. doi: 10.32592/ARI.2023.78.6.1709
Histopathological Evaluation of Eggshell and DBM Combination on the Repair of Critical Size Experimental Calvarial Bone Defects in Rats
1DVM Graduate, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4Assistant Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده
Fracture repair is a constant clinical challenge, and finding a method to promote and improve restoration is a primary goal for researchers. This is examined from various perspectives, such as fewer complications, increased speed, and cost-effectiveness. The present study aimed to investigate the effectiveness of eggshell powder, compared to the commercial form of demineralized bone matrix (DBM), in critical-size defects in rat calvarial bone. In this study, 40 adult male Wistar rats were selected and randomly divided into four groups of 10. The first group was the control group (C), the second was the eggshell powder group (E), the third was the DBM group (D), and the fourth was the one simultaneously receiving eggshell powder and DBM (DE). In these groups, a 5 mm diameter defect was created in the calvaria using a trephine. All animals received the appropriate treatment for their group. Each group was then divided into two subgroups of five. On days 30 and 60 post-surgery, these subgroups were euthanized, followed by sampling and histopathology examinations. After evaluating the repair percentage using Quick Photo software, the DE group had the highest repair percentage on days 30 and 60. Groups E and D had similar recovery percentages, with group D having a slightly higher one. There was a significant difference between all three groups and the control group. In conclusion, eggshell powder may potentially serve as a suitable substitute for some transplants.
ارزیابی هیستوپاتولوژی ترکیب Eggshell و DBM بر ترمیم نقیصه تجربی استخوان کالواریا با اندازه بحرانی در موش صحرایی
چکیده [English]
ترمیم شکستگی جزو چالشهای همیشگی بالینی میباشد و یافتن روشی برای ارتقاء و بهبود ترمیم از نکات اصلی مورد توجه محققان میباشد که از دیدگاههای مختلفی مانند عوارض کمتر، سرعت بیشتر و مقرون به صرفه بودن مورد بررسی قرار میگیرد. هدف از انجام مطالعه حاضر، بررسی اثر بخشی پودر پوسته تخم مرغ در مقایسه و به همراه فرم تجاری ماتریکس استخوانی دمینرالیزه در نقیصههای با اندازهی بحرانی در استخوان کالواریا موش صحرایی میباشد. در این تحقیق 40 سر موش صحرایی بالغ و نر از نژاد ویستار انتخاب شده و به صورت تصادفی در 4 گروه 10تایی تقسیم شدند. گروه اول کنترل (C)، گروه دوم گروه دریافت کننده پودر پوسته تخممرغ(E)، گروه سوم گروه دریافت کننده DBM(D) و گروه چهارم گروه دریافت کننده همزمان پودر پوسته تخم مرغ و DBM(DE) بودند. در این گروهها توسط یک ترفین نقیصهای بهقطر 5 میلیمتر در ناحیهی کالواریا ایجاد شد. تمامی حیوانات درمان متناسب با گروه خود را دریافت نمودند. سپس هر گروه به دو زیرگروه 5تایی تقسیم شدند و زیر گروه اول 30 روز و زیرگروه دوم 60 روز پس از جراحی، ابتدا آسانکشی و سپس نمونهگیری و بررسی هسیتوپاتولوژی انجام شد. پس از ارزیابی درصد ترمیم توسط نرمافزار QuickPhoto بیشترین درصد ترمیم در روزهای 30 و 60 را گروه DE داشت، گروههای E و D تقریباً درصد ترمیم مشابهی داشتند که گروه D اندکی بیشتر بود و هر سه گروه نسبت به گروه کنترل اختلاف معنیداری داشتند. در نهایت میتوان نتیجه گرفت که پودر پوستهی تخممرغ احتمالا میتواند جایگزین مناسبی برای بعضی از پیوندها باشد.
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