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Golchinfar, Fariba, Madani, Rasool, Hezarosi, Mahsa. (1402). Study the Profile of Foot-and-mouth Disease Virus Protein by Electrophoresis and Identification of the Immunodominant Proteins. سامانه مدیریت نشریات علمی, 78(5), 1563-1571. doi: 10.32592/ARI.2023.78.5.1563
Fariba Golchinfar; Rasool Madani; Mahsa Hezarosi. "Study the Profile of Foot-and-mouth Disease Virus Protein by Electrophoresis and Identification of the Immunodominant Proteins". سامانه مدیریت نشریات علمی, 78, 5, 1402, 1563-1571. doi: 10.32592/ARI.2023.78.5.1563
Golchinfar, Fariba, Madani, Rasool, Hezarosi, Mahsa. (1402). 'Study the Profile of Foot-and-mouth Disease Virus Protein by Electrophoresis and Identification of the Immunodominant Proteins', سامانه مدیریت نشریات علمی, 78(5), pp. 1563-1571. doi: 10.32592/ARI.2023.78.5.1563
Golchinfar, Fariba, Madani, Rasool, Hezarosi, Mahsa. Study the Profile of Foot-and-mouth Disease Virus Protein by Electrophoresis and Identification of the Immunodominant Proteins. سامانه مدیریت نشریات علمی, 1402; 78(5): 1563-1571. doi: 10.32592/ARI.2023.78.5.1563

Study the Profile of Foot-and-mouth Disease Virus Protein by Electrophoresis and Identification of the Immunodominant Proteins

Archives of Razi Institute
مقاله 17، دوره 78، شماره 5، آذر و دی 2023، صفحه 1563-1571    اصل مقاله (505.28 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2023.78.5.1563
نویسندگان
Fariba Golchinfar* 1؛ Rasool Madani2؛ Mahsa Hezarosi2
1Department of Proteomics and Biochemistry, Razi vaccine and serum research institute, Agricultural research education and extension organization (AREEO), Karaj, Iran
2Department of Proteomics and Biochemistry, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
چکیده
Foot-and-mouth disease is an extremely infectious and occasionally fatal viral disease with a rapid onset and a short course that affects cloven-hoofed animals and results in considerable financial losses. Today, Foot-and-mouth disease is controlled by traditional inactivated vaccines. Due to the short duration of immunity, a study was conducted for proteins of the virus as well as obtaining immunodominant proteins to design more efficient vaccines against Foot-and-mouth disease virus. This research aims to study the profile of Foot-and-mouth disease virus protein by electrophoresis and identification of the immunodominant proteins. The purified Foot-and-mouth disease virus was purchased then the protein concentration of that solution was measured by Lowry method. SDS-PAGE was done to achieve the protein profiles of the virus and immunization of 5 guinea pigs was done, then blood samples were taken for obtaining serum. Finally, serology tests; double immunodiffusion, ELISA, and western blotting were used to evaluate antigen response to antibodies (antigenic immunization). The protein concentration was 3.5 mg/ml. In SDS-PAGE with 10% gel, the protein profile of the virus was observed. After immunization, by conducting double immunodiffusion tests, the sediment lines between the serum antibody and the antigen of the virus were formed. Also, The ELISA test showed that the antibodies were formed against the antigens. In the western blot test, two immunodominant proteins of the FMD virus were obtained. According to the results, the immunodominant proteins of the FMD virus were determined. These proteins can be used in immunological diagnostic methods and also novel vaccines.
کلیدواژه‌ها
Double immunodiffusion؛ Electrophoresis؛ ELISA؛ Foot-and-mouth disease؛ Immunodominant proteins؛ Lowry method؛ Western blot
عنوان مقاله [English]
بررسی پروفایل الکتروفورزی پروتئین ویروس تب برفکی و شناسایی پروتئین‌های ایمونودومیننت ویروس تب برفکی
چکیده [English]
تب برفکی یک بیماری ویروسی بسیار عفونی و گهگاه کشنده با شروع سریع و دوره کوتاه است که بر حیوانات سم پاش تأثیر می گذارد و خسارات مالی قابل توجهی به دنبال دارد. امروزه بیماری تب برفکی با واکسن غیرفعال سنتی کنترل می شود. با توجه به کوتاه بودن مدت ایمنی، مطالعه ای برای پروتئین های ویروس و همچنین به دست آوردن پروتئین های ایمنی غالب برای طراحی واکسن های کارآمدتر علیه ویروس تب برفکی انجام شد. هدف از این تحقیق بررسی مشخصات پروتئین ویروس تب برفکی با الکتروفورز و شناسایی پروتئین‌های ایمنی غالب است.
ویروس تب برفکی خالص خریداری شد و غلظت پروتئین آن محلول به روش لوری اندازه‌گیری شد. برای دستیابی به پروفایل پروتئینی ویروس SDS-PAGE انجام شد و 5 خوکچه هندی ایمن سازی شدند، سپس نمونه خون برای تهیه سرم گرفته شد. در نهایت، آزمایشات سرولوژی؛ ایمونودیفیوژن دوبل، الایزا و وسترن بلات برای ارزیابی پاسخ آنتی ژن به آنتی بادی ها (ایمن سازی آنتی ژنی) استفاده شد.
غلظت پروتئین 3.5 میلی گرم بر میلی لیتر بود. در SDS-PAGE با ژل 10 درصد، مشخصات پروتئینی ویروس مشاهده شد. پس از ایمن سازی، با انجام تست ایمونودیفیوژن مضاعف، خطوط رسوبی بین آنتی بادی سرم و آنتی ژن ویروس تشکیل شد. همچنین آزمایش الایزا نشان داد که آنتی بادی ها علیه آنتی ژن ها تشکیل شده اند. در تست وسترن بلات، دو پروتئین ایمنی غالب از ویروس FMD بدست آمد.
با توجه به نتایج، پروتئین های ایمنی غالب ویروس FMD تعیین شد. از این پروتئین ها می توان در روش های تشخیص ایمونولوژیک و همچنین واکسن های جدید استفاده کرد.
کلیدواژه‌ها [English]
تب برفکی, سیستم ایمنی غالب, ایمونودیفیوژن, الکتروفورز, الایزا
سایر فایل های مرتبط با مقاله
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