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Razavi-Nikoo, Hadi, Behboudi, Emad, Aghcheli, Bahman, Hashemi, Seyed Mohammad Ali, Moradi, Abdolvahab. (1402). Bac to Bac system efficiency in HPV vaccine production. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2023.361975.2708
Hadi Razavi-Nikoo; Emad Behboudi; Bahman Aghcheli; Seyed Mohammad Ali Hashemi; Abdolvahab Moradi. "Bac to Bac system efficiency in HPV vaccine production". سامانه مدیریت نشریات علمی, , , 1402, -. doi: 10.22092/ari.2023.361975.2708
Razavi-Nikoo, Hadi, Behboudi, Emad, Aghcheli, Bahman, Hashemi, Seyed Mohammad Ali, Moradi, Abdolvahab. (1402). 'Bac to Bac system efficiency in HPV vaccine production', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2023.361975.2708
Razavi-Nikoo, Hadi, Behboudi, Emad, Aghcheli, Bahman, Hashemi, Seyed Mohammad Ali, Moradi, Abdolvahab. Bac to Bac system efficiency in HPV vaccine production. سامانه مدیریت نشریات علمی, 1402; (): -. doi: 10.22092/ari.2023.361975.2708

Bac to Bac system efficiency in HPV vaccine production

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 30 اردیبهشت 1402
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2023.361975.2708
نویسندگان
Hadi Razavi-Nikoo1؛ Emad Behboudi2؛ Bahman Aghcheli1؛ Seyed Mohammad Ali Hashemi3؛ Abdolvahab Moradi* 1
1Golestan University of Medical Sciences
2Khoy University of Medical Sciences
3Goletan University of Medical Sciences
چکیده
Today, the Human Papillomavirus (HPV) L1 protein is the main target in the construction of prophylactic HPV vaccines. The production of virus-like particles (VLPs) that closely resemble the natural structure of the HPV16 virus and induce high levels of virus-neutralizing antibodies in animals and humans is facilitated by the expression of HPV16-L1 protein in eukaryotic cells. The Bac to Bac system has been previously used to produce high levels of recombinant proteins. In this study, we utilized this expression system to generate HPV16-L1 VLPs in Sf9 insect cells.
The wild-type L1 gene of papillomavirus type 16 was selected from Gene Bank and after codon optimization using pFast Bac vector was placed in bacmid structure. The recombinant baculovirus containing HPV-16 / L1 gene was then provided using the Bac-to-Bac system. The vector was transfected into Sf9 cell. The cells were then lysed and the expression of L1 protein was examined by SDS-PAGE and Western Blot. VLP formation of papillomavirus L1 protein was evaluated by electron microscopy. The expressed recombinant L1 was ~60 KD on SDS-PAGE which by a specific anti-L1 monoclonal antibody was identified in western blot. Electron microscopy has confirmed the assembly of VLPs. This study showed that the production of this protein at the industrial level is optimized using a baculovirus/Sf9 system. The characteristics and advantages of this system make it a suitable candidate for large-scale protein synthesis.
کلیدواژه‌ها
HPV؛ L1؛ Sf9؛ baculovirus؛ vaccine
عنوان مقاله [English]
اثربخشی سیستم بیانی Bac to Bac در تولید واکسن پاپیلوما
آمار
تعداد مشاهده مقاله: 19


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