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شناسایی میکروارگانیسم‌های آنتاگونیست همراه فیلوسفر توت فرنگی و ارزیابی آنها در مهار زیستی Botrytis cinerea | ||
| مهار زیستی در گیاه پزشکی | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 09 اردیبهشت 1402 | ||
| نوع مقاله: مقاله علمی-پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2023.360936.328 | ||
| نویسندگان | ||
| نسرین سیدی1؛ حمیدرضا علیزاده1؛ مهدی آزادوار2؛ امیررضا امیرمیجانی* 3 | ||
| 1گروه گیاه پزشکی، دانشکدة کشاورزی، دانشگاه جیرفت | ||
| 2بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمان، کرمان، ایران | ||
| 3گروه گیاهپزشکی دانشکده کشاورزی دانشگاه جیرفت | ||
| چکیده | ||
| بیماری کپک خاکستری ناشی از Botrytis cinerea یکی از بیماری‌‌های مهم توتفرنگی است که باعث پوسیدگی میوه پیش و پس از برداشت محصول میشود. کنترل زیستی با استفاده از عوامل بیوکنترل یک روش ایمن و سالم برای کاهش خسارت این بیماری است. در این پژوهش با استفاده از روشهای مبتنی بر کشت، در مجموع 268 جدایه باکتریایی و 136 جدایه قارچی از فیلوسفر گیاهان توتفرنگی گلخانهای و فضای باز شهرستان جیرفت جداسازی و خالصسازی شد. اثر آنتاگونیستی جدایهها در برابر بیمارگر B. cinerea به روشهای کشت متقابل و بررسی هاله بازدارنده و اثر ترکیبات فرار در شرایط آزمایشگاه و همچنین خواص ضدقارچی باکتری‌های آنتاگونیست روی میوه توتفرنگی مورد بررسی قرار گرفت. شناسایی جدایههای باکتری و قارچی منتخب براساس واکنش زنجیرهای پلیمراز به ترتیب با تعیین توالی نوکلئوتیدی بخشی از ژن 16S–rDNA و ناحیه ITS–rDNA انجام گرفت. نتایج نشان داد که از بین جدایههای مورد بررسی جدایههای باکتریایی UJB1، UJB3، UJB4، UJB10، UJB5، UJB6، UJB7، UJB11، UJB2، UJB8 و UJB9 و جدایه قارچی UJF1300، UJF1301، UJF1302، UJF1303،UJF1304 ،UJF1305 اثر بازدارندگی موثری علیه B. cinereaداشتند و ترکیبات فرار آنها رشد پرگنه بیمارگر را کاهش داد. در ارزیابی اثر ضدقارچی جدایههای باکتری روی میوه توتفرنگی، جدایههای باکتریایی UJB8، UJB2، UJB11، UJB6، UJB5، UJB10، UJB4، UJB1 میزان شاخص بیماری ناشی از قارچ B. cinerea را به طور معنی‌داری نسبت به شاهد کاهش دادند. نتایج آنالیز دادههای حاصل ازتعیین توالی نوکلئوتیدی نشان داد که جدایههای باکتریایی متعلق به جنس‌های Delftia (جدایههای UJB1، UJB3، UJB4، UJB10)، Bacillus (جدایههای UJB5، UJB6، UJB7، UJB11 و UJB2) و Stenotrophomonas (جدایههای UJB8 و UJB9) و جدایههای قارچی متعلق به گونه‌های Albifimbria verrucaria، Aspergillus terreus، Leptosphaerulina australis، Pilidium lythri، Pseudozyma flocculosa و Seimatosporium pistaciae میباشند. | ||
| کلیدواژه‌ها | ||
| آنتاگونیست؛ بیوکنترل؛ جیرفت؛ 16S-rDNA؛ ITS-rDNA | ||
| عنوان مقاله [English] | ||
| Identification of antagonistic microorganisms associated with strawberry phyllosphere and their evaluation in the biological control of Botrytis cinerea | ||
| نویسندگان [English] | ||
| Nasrin Seyyedi1؛ Hamidreza Alizadeh1؛ Mehdi Azadvar2؛ Amirreza Amirmijani3 | ||
| 1Department of Plant Protection, Faculty of Agriculture, University of Jiroft, Jiroft, Iran | ||
| 2Department of Plant Protection, Kerman Agricultural and Natural Resources Research and Education Center, Tehran, Iran | ||
| 3Dept. of Plant Protection, Faculty of Agriculture, University of Jiroft | ||
| چکیده [English] | ||
| Gray mold, caused by Botrytis cinerea is one of the most important diseases in strawberry, causing pre– and postharvest fruit rot. One of the safe and healthy ways to reduce this disease is biocontrol and the use of biological control agents. In this study, using culture–based methods, 268 bacterial strains, and 136 fungal isolates, were isolated and purified from the phyllosphere of strawberry plants in the greenhouse and field in Jiroft city. The antagonistic effect of the isolates against the pathogen B. cinerea was investigated using dual culture and investigation of the inhibition zone, and the effect of volatile compounds under in vitro conditions, as well as the antifungal properties of the antagonistic bacteria on strawberry fruit. Identification of the selected bacterial and fungal strains was made by polymerase chain reaction, respectively, by determining the nucleotide sequence of a part of the 16S–rDNA gene and its region. The results showed that among the examined isolates, bacterial isolates UJB1, UJB3, UJB4, UJB10, UJB5, UJB6, UJB7, UJB11, UJB2, UJB8 and UJB9 and fungal isolates UJF1300, UJF1301, UJF1302, UJF1303, UJF1304, UJF1305 had an effective inhibitory effect against B. cinerea and their volatile compounds reduced pathogenic mycelium growth. In evaluating the antifungal impact of bacterial isolates on strawberry fruit, bacterial isolates UJB8, UJB2, UJB11, UJB6, UJB5, UJB10, UJB4, and UJB1 significantly increased the disease index caused by B. cinerea compared to They reduced the witness. According to morphological and molecular data, bacterial isolates belonging to the genera Delftia (isolates UJB1, UJB3, UJB4, UJB10), Bacillus (isolates UJB5, UJB6, UJB7, UJB11, and UJB2) and Stenotrophomonas (isolates UJB8 and UJB9) and fungal isolates belonging to Albifimbria verrucaria, Aspergillus terreus, Leptosphaerulina australis, Pilidium lythri, Pseudozyma flocculosa, and Seimatosporium pistaciae species. | ||
| کلیدواژه‌ها [English] | ||
| Antagonist, biological control, Jiroft, ITS-rDNA, 16S-rDNA | ||
| مراجع | ||
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