| تعداد نشریات | 283 |
| تعداد نشریات سازمان | 81 |
| تعداد شماره‌ها | 2,766 |
| تعداد مقالات | 31,497 |
| تعداد مشاهده مقاله | 39,694,192 |
| تعداد دریافت فایل اصل مقاله | 17,250,685 |
Morphological and molecular characterization of Podosphaera minor on Spiraea in Iran | ||
| Mycologia Iranica | ||
| مقاله 9، دوره 10، شماره 1، شهریور 2023، صفحه 103-108 اصل مقاله (1019.79 K) | ||
| نوع مقاله: Short Article | ||
| شناسه دیجیتال (DOI): 10.22043/MI.2023.361225.1249 | ||
| نویسندگان | ||
| S.A Khodaparast* ؛ H Darsaraei؛ M.J Pourmoghaddam | ||
| Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| چکیده | ||
| Powdery mildew is a common disease of Spiraea spp. worldwide; however, there are no reports of this destructive disease in Spiraea species from Iran. In recent years, severe powdery mildew symptoms have been observed in Spiraea spp. throughout the Guilan Province. In this study, we collected infected plant specimens and used morphological and molecular approaches to identify the powdery mildew fungus involved in this disease. We did not observe the teleomorphic state of the fungus in this area, and it appears that the fungus occurred only in the anamorphic state. At least six species of Podosphaera occur worldwide, and there is a considerable overlap between the anamorphic characteristics of some species. Hence, the ITS sequence was used to identify common powdery mildew species occurring in Spiraea in the Guilan Province. The results showed that the ITS sequence is useful for Podosphaera species in Spiraea. Podosphaera minor Howe. was identified as the causal agent of powdery mildew disease in this plant. To our knowledge, this is the first report of Podosphaera minor as a fungus in Iran. | ||
| کلیدواژه‌ها | ||
| Erysiphaceae؛ ITS-rDNA؛ Plant disease؛ Powdery mildew | ||
| مراجع | ||
|
Anonymous. 2023. eflora.org. Retrieved January 15, 2023, from shttp://www.efloras.org. Bradshaw M, Tobin PC. 2020. Sequencing herbarium specimens of a common detrimental plant disease (powdery mildew). Phytopathology 110: 1248– 1254. Braun U, Cook R. 2012. Taxonomic manual of the Erysiphales. Utrecht: CBS-KNAW Fungal Biodiversity Centre. Cunnington JH, Takamatsu S, Lawrie AC, Pascoe IG. 2003. Molecular identification of anamorphic powdery mildews (Erysiphales). Australasian Plant Pathology 32: 421–428. Darsaraei H, Khodaparast SA, Takamatsu S, Abbasi M, Asgari B, Sajedi S, Gotz M, Liu Sh, Feng J, Bradshaw M, Bulgakov T, Braun U. 2021. Phylogeny and taxonomy of the Erysiphe adunca complex (Erysiphaceae, Helotiales) on poplars and willows. Mycological Progress 20: 517–537. Khodaparast SA, Darsaraei H, Abbasi M. 2021. The genus Arthrocladiella: a new report of powdery mildew fungi from Iran. Mycologia Iranica 8: 135– 140. Farr DF, Rossman AY. 2023. Fungal Databases, U.S. National Fungus Collections, ARS, USDA. Retrieved January 15, 2023, from https://nt.arsgrin.gov/fungaldatabases. Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33: 1870–1874. Silvestro D, Michalak I. 2012. raxmlGUI: a graphical front-end for RAxML. Organisms Diversity & Evolution 12:335–337. Takamatsu S, Niinomi S, Harada M, Havrylenko M. 2010. Molecular phylogenetic analyses reveal a close evolutionary relationship between Podosphaera (Erysiphales: Erysiphaceae) and its rosaceous hosts. Persoonia-Molecular Phylogeny and Evolution of Fungi 24: 38–48. Zhang YJ, Zhang S, Liu X, Wen HA, Wang M. 2010. A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Letters in Applied Microbiology 51: 114–118 | ||
|
آمار تعداد مشاهده مقاله: 123 تعداد دریافت فایل اصل مقاله: 180 |
||