اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,049
تعداد مقالات34,082
تعداد مشاهده مقاله46,546,212
تعداد دریافت فایل اصل مقاله77,599,501
Torabi, S, Soleimani, S, Mahravani, H, Ebrahimi, M. M, Shahsavandi, S. (1402). Mouse Fibroblast L929 Cell Line as a Useful Tool for Replication and Adaptation of Infectious Bursal Disease Virus. سامانه مدیریت نشریات علمی, 78(3), 863-871. doi: 10.22092/ari.2023.361584.2663
S Torabi; S Soleimani; H Mahravani; M. M Ebrahimi; S Shahsavandi. "Mouse Fibroblast L929 Cell Line as a Useful Tool for Replication and Adaptation of Infectious Bursal Disease Virus". سامانه مدیریت نشریات علمی, 78, 3, 1402, 863-871. doi: 10.22092/ari.2023.361584.2663
Torabi, S, Soleimani, S, Mahravani, H, Ebrahimi, M. M, Shahsavandi, S. (1402). 'Mouse Fibroblast L929 Cell Line as a Useful Tool for Replication and Adaptation of Infectious Bursal Disease Virus', سامانه مدیریت نشریات علمی, 78(3), pp. 863-871. doi: 10.22092/ari.2023.361584.2663
Torabi, S, Soleimani, S, Mahravani, H, Ebrahimi, M. M, Shahsavandi, S. Mouse Fibroblast L929 Cell Line as a Useful Tool for Replication and Adaptation of Infectious Bursal Disease Virus. سامانه مدیریت نشریات علمی, 1402; 78(3): 863-871. doi: 10.22092/ari.2023.361584.2663

Mouse Fibroblast L929 Cell Line as a Useful Tool for Replication and Adaptation of Infectious Bursal Disease Virus

Archives of Razi Institute
مقاله 11، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 863-871    اصل مقاله (642.56 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2023.361584.2663
نویسندگان
S Torabi؛ S Soleimani؛ H Mahravani؛ M. M Ebrahimi؛ S Shahsavandi*
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده
Infectious bursal disease virus (IBDV) causes a highly contagious disease associated with immunosuppression in young chickens. Production of either egg-based or primary cell-based high-quality vaccines requires time-consuming and costly procedures. To determine a suitable cell line for IBDV replication, L929 cell line was a candidate for the growth kinetics processing of the virus. The L929 cells were proliferated in monolayer, and doubling time was calculated. Replication kinetics an IBDV isolate at the multiplicity of infection 0.1 PFU/cell were determined using virus titration. To adapt IBDV on L929 cells, seven consecutive passages were performed. Virus titer and levels of apoptosis were quantitatively analyzed at each passage. The viral VP2 gene was amplified and sequenced in three passages. An average doubling time of 21 h was estimated for monolayers of L929 cells. Although during early passages, virus growth did not produce a clear cytopathic effect (CPE), an increase in IBDV titers was observed. Serial passages led to the evidence of marked CPEs and an increase in the virus titer in the third passage. During the fourth to seventh passages, consistent CPEs characterized by the formation of granulated and round cells were evident within 24 to 48 hours post-inoculation. The titer of the virus was increased in the third passage onwards to peak in the fourth and constant at 5.9 TCID50 until the end passage. The IBDV replication in connection with DNA fragmentation and FITC, revealed the characteristic picture of apoptosis in a time-dependent manner. We found that the IBDV could easily be adapted to L929 cells, increasing virus yields by about two orders of magnitude. These results indicated that the cell line may be useful in the production of efficient virus particles.
کلیدواژه‌ها
Adaptation؛ Apoptosis؛ Infectious bursal disease virus؛ L929 cell line
سایر فایل های مرتبط با مقاله
مراجع
  1. Li J, Zheng S. Role of MicroRNAs in host defense against infectious bursal disease virus (IBDV) infection: a hidden front line. Viruses. 2020;12(5):543.
  2. Gimenez MC, Rodríguez Aguirre JF, Colombo MI, Delgui LR. Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a R ab5‐dependent manner. Cell Microbiol. 2015;17(7):988-1007.
  3. Qin Y, Zheng SJ. Infectious bursal disease virus-host interactions: multifunctional viral proteins that perform multiple and differing jobs. Int J Mol Sci. 2017;18(1):161.
  4. Qin Y, Xu Z, Wang Y, Li X, Cao H, Zheng S. VP2 of infectious bursal disease virus induces apoptosis via triggering oral cancer overexpressed 1 (ORAOV1) protein degradation. Front Microbiol. 2017;8:1351.
  5. Shahsavandi S, Ebrahimi MM, Sadeghi K, Mahravani H. Apoptotic response of chicken embryonic fibroblast cells to infectious bursal disease virus infections reflects viral pathogenicity. In Vitro Cell Dev Biol-Anim. 2014;50(9):858-64.
  6. Lawal Nu, Hair-Bejo M, Arshad SS, Omar AR, Ideris A. Adaptation and molecular characterization of two malaysian very virulent infectious bursal disease virus isolates adapted in BGM-70 cell line. Adv Virol. 2017;2017.
  1. Luo J, Zhang H, Teng M, Fan J-M, You L-M, Xiao Z-J, et al. Surface IgM on DT40 cells may be a component of the putative receptor complex responsible for the binding of infectious bursal disease virus. Avian Pathol. 2010;39(5):359-65.
  2. Rekha K, Sivasubramanian C, Chung I-M, Thiruvengadam M. Growth and replication of infectious bursal disease virus in the DF-1 cell line and chicken embryo fibroblasts. BioMed Res Int. 2014;2014.
  3. Lin T-W, Lo C-W, Lai S-Y, Fan R-J, Lo C-J, Chou Y-m, et al. Chicken heat shock protein 90 is a component of the putative cellular receptor complex of infectious bursal disease virus. J Virol. 2007;81(16):8730-41.
  4. Liu A, Pan Q, Li Y, Yan N, Wang J, Yang B, et al. Identification of chicken CD74 as a novel cellular attachment receptor for infectious bursal disease virus in bursa B lymphocytes. J Virol. 2020;94(2):e01712-19.
  5. Liu A, Pan Q, Wang S, Zhang Y, Li Y, Wang Y, et al. Identification of chicken CD44 as a novel B lymphocyte receptor for infectious bursal disease virus. J Virol. 2022;96(6):e00113-22.
  6. Ren X, Zhang L, Gao Y, Gao H, Wang Y, Liu C, et al. Binding chicken Anx2 is beneficial for infection with infectious bursal disease virus. Virus Res. 2015;210:232-40.
  7. Schneider-Schaulies J. Cellular receptors for viruses: links to tropism and pathogenesis. J Gen Virol. 2000;81(6):1413-29.
  8. Zhu LQ, Wu SL, Zhang GP, Zhu GQ. The cellular receptors for infectious bursal disease virus. Afr J Biotechnol. 2008;7(25).
  9. Chen C, Qin Y, Qian K, Shao H, Ye J, Qin A. HSC70 is required for infectious bursal disease virus (IBDV) infection in DF-1 cells. Virol J. 2020;17(1):1-11.
  10. Maddaluno L, Urwyler C, Rauschendorfer T, Meyer M, Stefanova D, Spörri R, et al. Antagonism of interferon signaling by fibroblast growth factors promotes viral replication. EMBO Mol Med. 2020;12(9):e11793.
  11. Kumar R, Khandelwal N, Thachamvally R, Tripathi BN, Barua S, Kashyap SK, et al. Role of MAPK/MNK1 signaling in virus replication. Virus Res. 2018;253:48-61.
  12. Ebrahimi MM, Shahsavandi S, Shayan P, Goudarzi H, Masoudi S. Recombinant VP2 expressed in baculovirus and adjuvanted with TIR-TLR7: a vaccine candidate against infectious bursal disease virus. Comp Clin Path. 2018;27:911-6.
  13. Genzel Y. Designing cell lines for viral vaccine production: Where do we stand?. Biotechnol J 2015;10(5):728-40.
  14. Jackwood DH, Saif Y, Hughes J. Replication of infectious bursal disease virus in continuous cell lines. Avian Dis.1987:370-5.
  15. Yip CW, Hon CC, Zeng F, Leung FCC. Cell culture-adapted IBDV uses endocytosis for entry in DF-1 chicken embryonic fibroblasts. Virus Res. 2012;165(1):9-16.
  16. Letzel T, Coulibaly F, Rey FA, Delmas B, Jagt E, Van Loon AA, et al. Molecular and structural bases for the antigenicity of VP2 of infectious bursal disease virus. J Virol. 2007;81(23):12827-35.
  17. Delgui L, Ona A, Gutierrez S, Luque D, Navarro A, Caston JR, et al. The capsid protein of infectious bursal disease virus contains a functional α4β1 integrin ligand motif. Virology. 2009;386(2):360-72.
  18. Ye C, Han X, Yu Z, Zhang E, Wang L, Liu H. Infectious bursal disease virus activates c-Src to promote α4β1 integrin-dependent viral entry by modulating the downstream Akt-RhoA GTPase-actin rearrangement cascade. J Virol. 2017;91(3):e01891-16.
  1. Shahsavandi S, Ebrahimi MM, Ghadiri MB, Samiee MR. Tween 80 improves the infectivity of BCL1 cell-adapted infectious bursal disease virus. J Virol Methods. 2022;304:114502.
  2. Rodríguez-Lecompte JC, Niño-Fong R, Lopez A, Markham RF, Kibenge FS. Infectious bursal disease virus (IBDV) induces apoptosis in chicken B cells. Comp Immunol Microbiol Infect Dis. 2005;28(4):321-37.
  3. Wei L, Hou L, Zhu S, Wang J, Zhou J, Liu J. Infectious bursal disease virus activates the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway by interaction of VP5 protein with the p85α subunit of PI3K. Virology. 2011;417(1):211-20.
  4. Qi X, Zhang L, Chen Y, Gao L, Wu G, Qin L, et al. Mutations of residues 249 and 256 in VP2 are involved in the replication and virulence of infectious bursal disease virus. PloS One. 2013;8(7):e70982.
آمار
تعداد مشاهده مقاله: 3,467
تعداد دریافت فایل اصل مقاله: 6,531


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب