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Evaluation of Molecular Epidemiology of IAPV in Several Regions of Iran | ||
| Archives of Razi Institute | ||
| مقاله 18، دوره 78، شماره 5، آذر و دی 2023، صفحه 1572-1578 اصل مقاله (439.97 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.32592/ARI.2023.78.5.1572 | ||
| نویسندگان | ||
| mojtaba moharrami* 1؛ Hossein Modirrousta2؛ Masoumeh Bagheri1 | ||
| 1Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, 31976-19751, Iran. | ||
| 2Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, 31976-19751, Iran.y | ||
| چکیده | ||
| In this study, the Israeli acute paralysis virus (IAPV), a single-stranded RNA virus, was investigated in honey bee colonies, which had a history of mortality, population decline, and parasitic diseases. Samples (adult honey bees) were collected from 328 apiaries from three provinces (Tehran, Alborz, and Mazandaran) of Iran to detect IAPV. After sample preparation, RNA was extracted and cDNA was synthesized to perform the reverse transcription polymerase chain reaction (RT-PCR) method using a PCR primer pair, and a 185 bp fragment was amplified. The results showed that out of 328 samples, 103 (31.4%) samples were positive, which were from Mazandaran (14.33%), Tehran (8.84%), and Alborz (8.23%) provinces. Subsequently, some of the positive samples were sequenced and a phylogenetic tree was drawn. The phylogenetic tree showed that the virus isolates were divided into two distinct groups, including one group that had a high similarity to the European acute bee paralysis virus (ABPV) and one group that had a high similarity to the Kashmir bee virus. In addition, the sequences of the samples in three regions were separated in a node from the strains of ABPV from Eastern Europe. Since the length of the branch between the Iranian sequences and the different strains of ABPV from Eastern Europe was short, it can be assumed that the sequences from Iran have a common ancestor with the mentioned strains of ABPV from Eastern Europe. | ||
| کلیدواژهها | ||
| Honey bee؛ IAPV؛ Phylogenetic tree؛ RT-PCR | ||
| عنوان مقاله [English] | ||
| اپیدمیولوژی مولکولی IAPV در زنبورستانهای سه استان ایران | ||
| چکیده [English] | ||
| در این مطالعه، ویروس فلجی اسرائیلی به عنوان یک ویروس RNA تک رشتهای، در کلنیهای زنبور عسل که سابقه مرگ و میر، کاهش جمعیت و بیماریهای انگلی داشتند، مورد بررسی قرار گرفت. در مجموع 328 نمونه زنبورستان بالغ از سه استان ایران (تهران، البرز و مازندران) برای تشخیص IAPV جمع آوری شد. پس از آمادهسازی نمونهها، RNA استخراج و cDNA برای انجام روش RT-PCR سنتز شد و قطعه 185 جفت باز تکثیر شد. نتایج نشان داد که از مجموع 328 نمونه، 103 نمونه (4/31%) مثبت ارزیابی شدند که سهم هریک از استانهای تهران معادل 84/8%، البرز معادل 23/8% و مازندران معادل 33/14% بود. سپس تعدادی از نمونه های مثبت توالی یابی شده و درخت فیلوژنتیک ترسیم شد. درخت فیلوژنتیک نشان داد که جدایههای ویروس به دو گروه مختلف تقسیم شدند، گروهی که شباهت زیادی به ویروس فلجی حاد زنبور عسل اروپایی داشتند و گروهی که شباهت زیادی با ویروس زنبور عسل کشمیر داشتند. همچنین، توالیهایی از نمونهها در سه منطقه در یک گره از سویههای ABPV از اروپای شرقی جدا شدند. با توجه به اینکه طول شاخه بین توالیهای ایرانی و سویههای مختلف ABPV از اروپای شرقی کوتاه بود، میتوان نتیجه گرفت که توالیهای ایران جد مشترکی با سویههای مذکور در ABPV از اروپای شرقی دارند. | ||
| کلیدواژهها [English] | ||
| زنبور عسل, IAPV RT-PCR درخت فیلوژنی | ||
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| مراجع | ||
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References 1.Calderone NW. Insect pollinated crops, insect pollinators and US agriculture: Trend analysis of aggregate data for the period 1992- 2009. PLoS One. 2012;7: e37235. 2.Shah KS. Localization of Deformed Wing Virus (DWV) in the Brains of Apis mellifera (European Honey Bees). Honor’s Theses, Bucknell University. 2009;19. 3.Mihara T, Koyano H, Hingamp P, Grimsley, N, Goto, S, Ogata, H. Taxon richness of megaviridae exceeds those of bacteria and archaea in the ocean. Microbes Environ. 2018; 33:162–171. 4.Shi M, Lin X, Dm Tian JH, Chen LJ, Chen X, Li CX, Qin XC, Li J, Cao JP, Eden JS, et al. Redefining the invertebrate RNA virosphere. Nat Cell Biol. 2016; 540:539–543. 5.Castelli L, Genchi García ML, Dalmon A, Arredondo D, Antúnez K, Invernizzi C, Reynaldi FJ, Le Conte Y, Beaurepaire A. IntraColonial Viral Infections in Western Honey Bees (Apis Mellifera). Microorganisms. 2021;9(5):1087. 6.Brødsgaard CJ, Ritter W, Hansen H, Brødsgaard HF. Interactions among Varroa jacobsoni mites, acute paralysis virus, and Paenibacillus larvae larvae and their influence on mortality of larval honeybees in vitro. Apidologie. 2000; 31:543-554. 7.Maori E, Lavi S, Mozes-Koch R, Gantman Y, Peretz Y, Edelbaum O, Tanne E, Sela I. Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra-and inter-species recombination. J Gen Virol. 2007; 88:3428-3438. 8.Chen YP, Pettis JS, Corona M, Chen WP, Li CJ, Spivak M, and et al. Israeli acute paralysis virus: epidemiology, pathogenesis and implications for honey bee health. PLOS Pathog. 2014;10 :7 9.Cox-Foster DL, Conlan S, Holmes EC, Palacios G, Evans JD, et al. Ametagenomic survey of microbes in honey bee colony collapse disorder. Science. 2007; 318:283-287. 10. Blanchard P, Schurr F, Celle O, Cougoule N, Drajnudel P, Thiéry R, Faucon JP, Ribière M. First detection of Israeli acute paralysis virus (IAPV) in France, a dicistro virus affecting honeybees (Apis mellifera). J Invertebr Pathol. 2008;99: 348-50. 11. Yan X, Liu XJ, Chen JH, Zhang Y, Han RC. First detection of Israel acute paralysis virus (IAPV) in Apis mellifera (Hymenoptera: Apidae) in China. Sociobiology. 2009; 95-100. 12. Reynaldi FJ, Sguazza GH, Tizzano MA, Fuentealba N, Galosi CM, Pecoraro MR. First report of Israeli acute paralysis virus in asymptomatic hives of Argentina. Revista Argentina de Microbiología. 2011; 43:84-86. 13. Mayra C, Alejandro RC, Alma I, Gerardo PO, Carlos H. Detection of Israeli Acute Paralysis Virus (IAPV) and Apis Apis mellifera Filamentous Virus (AmFV) in Honey Bees in Mexico. J Apic Sci. 2018;62(1):141-144. 14. Johnson KN, Christian PD. Molecular characterization of Drosophila C virus isolates. J Invertebr Pathol. 1999;73: 248-254. 15. Berenyi O, Bakonyi T, Derakhshifar I, Koglberger H, Nowotny N. Occurrence of six honeybee's viruses in diseased Austrian apiaries. Appl Environ Microbiol. 2006;2414-2420. 16. VanEngelsdorp D, Underwood R, Caron D, Hayes Jr J. An estimate of managed colony losses in the winter of 2006– 2007: A report commissioned by the apiary inspectors of America. Am Bee J. 2007;147: 599–603. 17. Pettis JS, Lichtenberg EM, Andree M, Stitzinger J, Rose R, vanEngelsdorp D. Crop Pollination Exposes Honey Bees to Pesticides Which Alters Their Susceptibility to the Gut Pathogen Nosema ceranae. PLoS One. 2013;8: e70182. 18. Alaux C, Brunet JL, Dussaubat C, Mondet F, Tchamitchan S, Cousin M, Brillard J,3Aurelie Baldy,1Luc P. Belzunces2and Yves Le Conte1 Interactions between Nosema microspores and a neonicotinoid weaken honeybees (Apis mellifera). Environ Microbiol. 2010; 12:774-782. 19. Deng Y, Yang S, Zhao H, Diao Q, Hou C. IAPVInduced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function. Int J Mol Sci. 2021; 22(18):10078. 20. Bhatia S, Baral SS, Vega Melendez C, Amiri E, Rueppell O. Comparing Survival of Israeli Acute Paralysis Virus Infection among Stocks of U.S. Honey Bees. Insects. 2021;12(1):60. 21. Truong AT, Kim B, Kim S, Kim M, Kim J, Kim S, Yoon B. Rapid detection of Israeli acute paralysis virus using multipoint ultra-rapid real-time PCR (UR-qPCR). J Apic Res. 2019; 58:746–53. 22. Wang H, Meeus I, Piot N, Smagghe G. Systemic Israeli acute paralysis virus (IAPV) infection in bumblebees (Bombus terrestris) through feeding and injection, J Invertebr Pathol. 2018; 151:158-164. | ||
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