Fowl Adenoviruses (FAdVs) are widely distributed pathogens across the globe. The FAdVs from serotypes FAdV 2, 3, 8a, 8b, 9, and 11 are responsible for inclusion body hepatitis (IBH). Recently, increased mortality and IBH-suspected lesions were observed in 8-10-day-old broiler chickens in West Azerbaijan Province, Iran. In this regard, the present study aimed to compare penton and hexon genes of ADDV11 in the molecular detection of IBH in broiler chickens. In total, 100 liver specimens were collected from 10 suspected farms, and their DNAs were extracted. Two polymerase chain reactions (PCRs) were applied; one targeting the L1 region of the hexon gene and another aiming at the penton gene. Based on the findings, 60% of samples showed positive results in both PCRs and phylogenetic analysis clustered the studied viruses into serotype 11 (species D) FAdV. The detected FAdVs also shared a multitude of homologies with previously published serotype 11 viruses from Iran and those identified in Pakistan, Saudi Arabia, India, China, and Canada. This research not only provides an update on circulating FAdVs in Iran, but also introduces the penton gene as an alternative target for IBH diagnosis. Considering that IBH is a primary disease in Iran with both horizontal and vertical routes of transmission, urgent preventive measures are needed. |
- Kaján GL, Affranio I, Bistyák AT, Kecskeméti S, Benkő M. An emerging new fowl adenovirus genotype. Heliyon. 2019;5(5):e01732.
- Zadravec M, Slavec B, Krapež U, Kajan G, Račnik J, Juntes P, et al. Inclusion body hepatitis (IBH) outbreak associated with fowl adenovirus type 8b in broilers. Acta Vet. 2013;63(1):101-10.
- Gupta A, Ahmed KA, Ayalew LE, Popowich S, Kurukulasuriya S, Goonewardene K, et al. Immunogenicity and protective efficacy of virus-like particles and recombinant fiber proteins in broiler-breeder vaccination against fowl adenovirus (FAdV)-8b. Vaccine. 2017;35(20):2716-22.
- Schachner A, Matos M, Grafl B, Hess M. Fowl adenovirus-induced diseases and strategies for their control – a review on the current global situation. Avian Pathol. 2018;47(2):111-26.
- Steer PA, Kirkpatrick NC, O'Rourke D, Noormohammadi AH. Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region. J Clin Microbiol. 2009;47(2):311-21.
- Helmboldt CF, Frazier MN. Avian Hepatic Inclusion Bodies of Unknown Significance. Avian Dis. 1963;7(4):446-50.
- Liu Y, Wan W, Gao D, Li Y, Yang X, Liu H, et al. Genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in China. Emerg Microbes Infect. 2016;5(1):1-8.
- Lim T-H, Kim B-Y, Kim M-S, Jang J-H, Lee D-H, Kwon Y-K, et al. Outbreak of gizzard erosion associated with fowl adenovirus infection in Korea. Poultry Sci. 2012;91(5):1113-7.
- Shah M, Ashraf A, Khan M, Rahman M, Habib M, Qureshi J. Molecular cloning, expression and characterization of 100K gene of fowl adenovirus-4 for prevention and control of hydropericardium syndrome. Biologicals. 2016;44(1):19-23.
- Park H-S, Lim I-S, Kim S-K, Kim T-K, Park C-K, Yeo S-G. Molecular analysis of the hexon, penton base, and fiber-2 genes of Korean fowl adenovirus serotype 4 isolates from hydropericardium syndrome-affected chickens. Virus Genes. 2017;53(1):111-6.
- Wang J, Zaheer I, Saleemi MK, Qi X, Gao Y, Cui H, et al. The first complete genome sequence and pathogenicity characterization of fowl adenovirus 11 from chickens with inclusion body hepatitis in Pakistan. Vet Microbiol. 2020;244:108670.
- Hosseini H, Langeroudi AG, FallahMehrabadi MH, Kafi ZZ, Dizaji RE, Ghafouri SA, et al. The fowl adenovirus (Fadv-11) outbreak in Iranian broiler chicken farms: The first full genome characterization and phylogenetic analysis. Comp Immunol Microbiol Infect Dis. 2020;70:101365.
- Chitradevi S, Sukumar K, Suresh P, Balasubramaniam G, Kannan D. Molecular typing and pathogenicity assessment of fowl adenovirus associated with inclusion body hepatitis in chicken from India. Trop Anim Health Prod. 2021;53(4):1-12.
- Jiang P, Ojkic D, Tuboly T, Huber P, Nagy E. Application of the polymerase chain reaction to detect fowl adenoviruses. Can J Vet Res. 1999;63(2):124.
- El-Tholoth M, El-Azm A, Kamel I. Molecular detection and characterization of fowl adenovirus associated with inclusion body hepatitis from broiler chickens in Egypt. Trop Anim Health Prod. 2019;51(5):1065-71.
- Fadly A, Riegle B, Nazerian K, Stephens E. Some observations on an adenovirus isolated from specific pathogen free chickens. Poultry Sci. 1980;59(1):21-7.
- Niczyporuk JS. Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland. Arch Virol. 2016;161(1):33-42.
- Hosseini H, Morshed R. Molecular identification of fowl adenovirus associated with inclusion body hepatitis in Iran. Iran J Virol. 2012;6(4):7-12.
- Morshed R, Hosseini H, Langeroudi AG, Fard MHB, Charkhkar S. Fowl adenoviruses D and E cause inclusion body hepatitis outbreaks in broiler and broiler breeder pullet flocks. Avian Dis. 2017;61(2):205-10.
- Arazi S, Mayahi M, Talazadeh F, Boroomand Z, Jafari R. Molecular identification of aviadenoviruses in broiler chickens suspected to inclusion body hepatitis in Golestan province, Iran. Iran J Vet Res. 2020;21(3):198.
- Najafi H, FallahMehrabadi MH, Hosseini H, Kafi ZZ, Hamdan AM, Ghalyanchilangeroudi A. The first full genome characterization of an Iranian foot and mouth disease virus. Virus Res. 2020;279:197888.
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