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Shahveh, Mahmoud, Tajbakhsh, Elahe, Momtaz, Hassan, Ranjbar, Reza. (1401). Molecular characterization of Enterococcus faecalis and Enterococcus faecium isolated from a meat source in Shahrekord local markets, Iran. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2022.360355.2575
Mahmoud Shahveh; Elahe Tajbakhsh; Hassan Momtaz; Reza Ranjbar. "Molecular characterization of Enterococcus faecalis and Enterococcus faecium isolated from a meat source in Shahrekord local markets, Iran". سامانه مدیریت نشریات علمی, , , 1401, -. doi: 10.22092/ari.2022.360355.2575
Shahveh, Mahmoud, Tajbakhsh, Elahe, Momtaz, Hassan, Ranjbar, Reza. (1401). 'Molecular characterization of Enterococcus faecalis and Enterococcus faecium isolated from a meat source in Shahrekord local markets, Iran', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2022.360355.2575
Shahveh, Mahmoud, Tajbakhsh, Elahe, Momtaz, Hassan, Ranjbar, Reza. Molecular characterization of Enterococcus faecalis and Enterococcus faecium isolated from a meat source in Shahrekord local markets, Iran. سامانه مدیریت نشریات علمی, 1401; (): -. doi: 10.22092/ari.2022.360355.2575

Molecular characterization of Enterococcus faecalis and Enterococcus faecium isolated from a meat source in Shahrekord local markets, Iran

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مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 07 اسفند 1401  XML
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.360355.2575
نویسندگان
Mahmoud Shahveh1؛ Elahe Tajbakhsh email 2؛ Hassan Momtaz1؛ Reza Ranjbar3
1Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
3Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
چکیده
Enterococcus faecalis (E. faecalis) and E. faecium (E. faecium) are commensals of the gastrointestinal biota of humans and animals and are considered opportunistic pathogens. This study aimed to improve knowledge about E. faecalis and E. faecium isolated from meat. Thus, 104 meat samples from sheep, goats, cattle, and calves were collected from Shahrekord local markets. Presumptive colonies of E. faecalis and E. faecium were isolated from the samples and subjected to identification tests. Antimicrobial susceptibility was determined using the Kirby–Bauer disc diffusion method. PCR was performed to detect some virulence genes. RAPD typing and REP-PCR fingerprinting were used to show the clonal relatedness of isolates. The results revealed that enterococci were present in 90 out of 104 samples. E. faecalis and E. faecium were the commonly isolated species, with a predominance of E. faecalis. E. faecalis exhibited high resistance to streptomycin (95%) but is susceptible to vancomycin 85.6%. Virulence genes detection shows that ccf and cpd genes are the most found for both species. The molecular typing method indicated that the isolates belonged to separate subgroups. This study shows contamination of meat products by potential pathogens and resistant enterococci. There is a need to implement regular surveillance to monitor the emergence of antimicrobial-resistant E. faecalis and E. faecium in food, particularly meat production.
کلیدواژه‌ها
Gastrointestinal biota؛ pathogens؛ meat؛ antimicrobial susceptibility؛ PCR
عنوان مقاله [English]
خصوصیات مولکولی انتروکوکوس فکالیس و انتروکوکوس فاسیوم جدا شده از منابع گوشتی فروشگاه های شهرکرد
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