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Alimolaei, Mojtaba, Shamsaddini Bafti, Mehrdad. (1401). Isolation and molecular characterization of Clostridium perfringens toxinotypes F & G in diarrhoeic sheep (Ovis aries) flocks in southeast Iran. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2023.360450.2582
Mojtaba Alimolaei; Mehrdad Shamsaddini Bafti. "Isolation and molecular characterization of Clostridium perfringens toxinotypes F & G in diarrhoeic sheep (Ovis aries) flocks in southeast Iran". سامانه مدیریت نشریات علمی, , , 1401, -. doi: 10.22092/ari.2023.360450.2582
Alimolaei, Mojtaba, Shamsaddini Bafti, Mehrdad. (1401). 'Isolation and molecular characterization of Clostridium perfringens toxinotypes F & G in diarrhoeic sheep (Ovis aries) flocks in southeast Iran', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2023.360450.2582
Alimolaei, Mojtaba, Shamsaddini Bafti, Mehrdad. Isolation and molecular characterization of Clostridium perfringens toxinotypes F & G in diarrhoeic sheep (Ovis aries) flocks in southeast Iran. سامانه مدیریت نشریات علمی, 1401; (): -. doi: 10.22092/ari.2023.360450.2582

Isolation and molecular characterization of Clostridium perfringens toxinotypes F & G in diarrhoeic sheep (Ovis aries) flocks in southeast Iran

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مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 07 اسفند 1401  XML
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2023.360450.2582
نویسندگان
Mojtaba Alimolaei email orcid 1؛ Mehrdad Shamsaddini Bafti2
1Research and Development Department, Kerman branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran
2Department of Anaerobic Bacterial Vaccine Research and Production, Kerman branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran
چکیده
Clostridial enteric diseases, called enterotoxemia, are caused by Clostridium perfringens (C. perfringens) toxinotypes in sheep and other ruminants. This study describes the molecular characterization of C. perfringens isolates in diarrhoeic sheep (Ovis aries) flocks in the southeast of Iran. Faecal/intestinal samples were collected from diarrhoeic (n=116), dead (n= 13), and healthy (n=63) sheep over four years (2016-2020) and subjected to bacteriological and molecular examinations. C. perfringens isolates were typed by PCR targeting genes viz. 16SrRNA, cpa, cpb, etx, iap, cpe, and netb. The overall prevalence of C. perfringens was 28.6% among sheep, and there was a significant relationship between its isolation rate and diarrhoea (p<0.001). C. perfringens isolation rate also decreased with animal age (p=0.012) and was significantly higher in late winter and spring (p=0.000). The most prevalent toxinotypes were types A (52.4%), D (22.2%), and F (18.5%), respectively. Among the others, 4.2%, 1.6%, and 1.1% were assigned to C, G, and B types, respectively, and no type E was detected. The cpe gene was detected in 32.3% of all isolates, and the diarrhoeic sheep were most likely to yield cpe+ strains of C. perfringens (93.1%). These findings highlight the importance of cpe+ strains of C. perfringens in sheep enteritis and suggest that the high presence of type F needs to develop in new clostridial vaccines containing this toxinotype. Interestingly, we report the isolation of C. perfringens type F, type G, and the cpe+ strains of type B from diarrhoeic sheep for the first time.
کلیدواژه‌ها
Clostridium perfringens؛ Toxinotype؛ Diarrhoea؛ Sheep (Ovis aries)؛ Iran
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