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بررسی خواص آنتی‌اکسیدانی و سیتوتوکسیک عصاره هیدروالکلی Ephedra major Host. بر رده سلولی AGS سرطان معده | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 7، دوره 39، شماره 2، تیر 1402، صفحه 224-236 اصل مقاله (816.89 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2023.357854.3143 | ||
| نویسندگان | ||
| فرهنگ مراقبی* 1؛ شیدا آقازاده یامچلو2؛ سیده مهدخت مداح2 | ||
| 1عضو هیات علمی دانشگاه آزاد اسلامی واحد شهر ری | ||
| 2گروه علوم و فنون زیستی، واحد یادگار امام خمینی (ره) شهرری، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده | ||
| سرطان معده یک تومور بدخیم است که یکی از شایع‌ترین انواع سرطان در جهان و ایران شناخته می‌شود. گیاهان منابعی سرشار از انواع ترکیب‌های آنتی‌اکسیدانی می‌باشند که می‌توانند در جهت تولید داروهای ضد سرطانی استفاده شوند. در این پژوهش خواص آنتی‌اکسیدانی و اثر سمّیت سلولی عصاره گیاه Ephedra major Host.، جمع‌آوری شده از پرندک در شهرستان رباط کریم، استان تهران روی سلولهای سرطان معده ردهAGS بررسی شد. عصاره هیدروالکلی اتانولی و متانولی (80%) سرشاخه‌های رویشی گیاه به روش خیساندن استخراج شد. محتوی فنلی عصاره‌ها با عامل Folin-Ciocalteu و فعالیت آنتی‌اکسیدانی آنها با استفاده از تست DPPH مورد سنجش قرار گرفت. همچنین میزان سمّیت سلولی غلظت‌های مختلف عصاره این گیاه روی سلولهای رده AGS سرطان معده در سه زمان 24، 48 و 72 ساعت با استفاده از روش MTT ارزیابی شد. میزان تغییر بیان ژن BCL2 با استفاده از روش Real time-PCR اندازه‌‌گیری شد. نتایج نشان داد، خاصیت آنتی‌اکسیدانی و محتوای فنولی عصاره اتانولی نسبت به عصاره متانولی بیشتر بود. نتایج تست MTT نشان داد که با افزایش غلظت عصاره اتانولی، زنده‌مانی سلول‌ها کمتر شده و پس از 48 ساعت، IC50 به میزان 250 میکروگرم در میلی‌لیتر عصاره بدست آمد. میزان بیان ژن BCL2 در تیمار با عصاره کاهش یافت هر چند این کاهش معنی‌دار نبود. به‌طور کلی، عصاره اتانولی سرشاخه‌های هوایی این گیاه توانست موجب مهار رشد سلولهای سرطان معده گردد، اما تأثیر معنی‌داری بر کاهش بیان ژن ضد آپوپتوزی BCL2 نداشت. | ||
| کلیدواژه‌ها | ||
| Ephedra major Host؛ بیان ژن BCL-2؛ سرطان معده؛ رده سلولی AGS؛ آپوپتوز | ||
| عنوان مقاله [English] | ||
| Study on antioxidant and cytotoxic properties of Ephedra major Host. hydroalcoholic extract on AGS gastric carcinoma cell line | ||
| نویسندگان [English] | ||
| F. Moraghebi1؛ Sh. Aghazadeh Yamchelo2؛ S.M. Maddah2 | ||
| 2Department of Biological Sciences and Technologies, Faculty of member, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| Gastric cancer is a malignant tumor that is one of the most common types of cancer in the world and Iran. Plants are rich sources of a variety of antioxidant compounds which can be used to produce anti-cancer drugs. In this study, the antioxidant properties and cytotoxicity of Ephedra major Host. extract, collected from "Parandak" in Robat Karim city, Tehran province, were investigated on AGS gastric cancer cells. Ethanol and methanol hydroalcoholic extracts (80%) of plant vegetative shoots were extracted by soaking method. Phenolic content of the extracts was measured by Folin-Ciocalteu agent and their antioxidant activity was assessed using DPPH test. Also, the cytotoxicity degree of different concentrations of this plant extract on the AGS gastric cancer cells was evaluated at three times of 24, 48, and 72 hours using MTT method. BCL2 gene expression change was measured using Real time-PCR. The results showed that the antioxidant properties and phenolic content of ethanol extract were higher than methanol one. The results of MTT test showed that with increasing concentration of ethanol extract, cell viability decreased and after 48 hours, IC50 was obtained 250 µg.ml-1 of extract. The BCL2 gene expression decreased in the extract treatment, although this decrease was not significant. Overall, the ethanol extract of the aerial shoots of this plant could inhibit the growth of gastric cancer cells, but did not have a significant effect on reducing the expression of BCL2 anti-apoptotic gene. | ||
| کلیدواژه‌ها [English] | ||
| Ephedra major Host, BCL2 gene expression, Gastric cancer, AGS cell line, Apoptosis | ||
| مراجع | ||
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