| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,846 |
| تعداد مقالات | 32,300 |
| تعداد مشاهده مقاله | 41,386,715 |
| تعداد دریافت فایل اصل مقاله | 18,562,051 |
بررسی خواص آنتی‌اکسیدانی و سیتوتوکسیک عصاره هیدروالکلی Ephedra major Host. بر رده سلولی AGS سرطان معده | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 7، دوره 39، شماره 2، تیر 1402، صفحه 224-236 اصل مقاله (816.89 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2023.357854.3143 | ||
| نویسندگان | ||
| فرهنگ مراقبی* 1؛ شیدا آقازاده یامچلو2؛ سیده مهدخت مداح2 | ||
| 1عضو هیات علمی دانشگاه آزاد اسلامی واحد شهر ری | ||
| 2گروه علوم و فنون زیستی، واحد یادگار امام خمینی (ره) شهرری، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده | ||
| سرطان معده یک تومور بدخیم است که یکی از شایع‌ترین انواع سرطان در جهان و ایران شناخته می‌شود. گیاهان منابعی سرشار از انواع ترکیب‌های آنتی‌اکسیدانی می‌باشند که می‌توانند در جهت تولید داروهای ضد سرطانی استفاده شوند. در این پژوهش خواص آنتی‌اکسیدانی و اثر سمّیت سلولی عصاره گیاه Ephedra major Host.، جمع‌آوری شده از پرندک در شهرستان رباط کریم، استان تهران روی سلولهای سرطان معده ردهAGS بررسی شد. عصاره هیدروالکلی اتانولی و متانولی (80%) سرشاخه‌های رویشی گیاه به روش خیساندن استخراج شد. محتوی فنلی عصاره‌ها با عامل Folin-Ciocalteu و فعالیت آنتی‌اکسیدانی آنها با استفاده از تست DPPH مورد سنجش قرار گرفت. همچنین میزان سمّیت سلولی غلظت‌های مختلف عصاره این گیاه روی سلولهای رده AGS سرطان معده در سه زمان 24، 48 و 72 ساعت با استفاده از روش MTT ارزیابی شد. میزان تغییر بیان ژن BCL2 با استفاده از روش Real time-PCR اندازه‌‌گیری شد. نتایج نشان داد، خاصیت آنتی‌اکسیدانی و محتوای فنولی عصاره اتانولی نسبت به عصاره متانولی بیشتر بود. نتایج تست MTT نشان داد که با افزایش غلظت عصاره اتانولی، زنده‌مانی سلول‌ها کمتر شده و پس از 48 ساعت، IC50 به میزان 250 میکروگرم در میلی‌لیتر عصاره بدست آمد. میزان بیان ژن BCL2 در تیمار با عصاره کاهش یافت هر چند این کاهش معنی‌دار نبود. به‌طور کلی، عصاره اتانولی سرشاخه‌های هوایی این گیاه توانست موجب مهار رشد سلولهای سرطان معده گردد، اما تأثیر معنی‌داری بر کاهش بیان ژن ضد آپوپتوزی BCL2 نداشت. | ||
| کلیدواژه‌ها | ||
| Ephedra major Host؛ بیان ژن BCL-2؛ سرطان معده؛ رده سلولی AGS؛ آپوپتوز | ||
| عنوان مقاله [English] | ||
| Study on antioxidant and cytotoxic properties of Ephedra major Host. hydroalcoholic extract on AGS gastric carcinoma cell line | ||
| نویسندگان [English] | ||
| F. Moraghebi1؛ Sh. Aghazadeh Yamchelo2؛ S.M. Maddah2 | ||
| 2Department of Biological Sciences and Technologies, Faculty of member, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| Gastric cancer is a malignant tumor that is one of the most common types of cancer in the world and Iran. Plants are rich sources of a variety of antioxidant compounds which can be used to produce anti-cancer drugs. In this study, the antioxidant properties and cytotoxicity of Ephedra major Host. extract, collected from "Parandak" in Robat Karim city, Tehran province, were investigated on AGS gastric cancer cells. Ethanol and methanol hydroalcoholic extracts (80%) of plant vegetative shoots were extracted by soaking method. Phenolic content of the extracts was measured by Folin-Ciocalteu agent and their antioxidant activity was assessed using DPPH test. Also, the cytotoxicity degree of different concentrations of this plant extract on the AGS gastric cancer cells was evaluated at three times of 24, 48, and 72 hours using MTT method. BCL2 gene expression change was measured using Real time-PCR. The results showed that the antioxidant properties and phenolic content of ethanol extract were higher than methanol one. The results of MTT test showed that with increasing concentration of ethanol extract, cell viability decreased and after 48 hours, IC50 was obtained 250 µg.ml-1 of extract. The BCL2 gene expression decreased in the extract treatment, although this decrease was not significant. Overall, the ethanol extract of the aerial shoots of this plant could inhibit the growth of gastric cancer cells, but did not have a significant effect on reducing the expression of BCL2 anti-apoptotic gene. | ||
| کلیدواژه‌ها [English] | ||
| Ephedra major Host, BCL2 gene expression, Gastric cancer, AGS cell line, Apoptosis | ||
| مراجع | ||
|
- Abdalan, S., Baghbani-Arani, F. and Shandiz, A., 2018. Evaluation of anticancer effect of aqueous and hydroalcoholic extracts of Quercus infectoria leaf against colon cancer HT29 cell line. Journal of Arak University of Medical Sciences, 21(4): 48-56. - Aghdasi, M., Mofid Bojnourdi, M., Mianabadi, M. and Nadaf, M., 2016. Chemical components of the Ephedra major from Iran. Natural product Letters, 30(3): 369-371. - Asadi, M., 1997. Flora of Iran: Ephedraceae (Volume 22). Publications of Forest and Rangeland Research Institute. - Ataiee Azimi, O., Delnavaz Hashemloyan, B. and Amirnia, A., 2017. Comparison of alkaloids, phenol and antioxidant activity of natural Middle Armak with isolated in vitro cultures. Plant Process and Function, 21(6): 143-152. - Azimi, Y., Maddah, S.M. and Mostafavi, G., 2020. The comparative study of the antioxidant activity of aqueous and hydroalcoholic extracts of an Iranian endemic species Rhabdosciadium aucheri Boiss. Iranian Journal of Plant Physiology, 11(3): 3701-3708. - Biagioni, A., Skalamera, I., Peri, S., Schiavone, N., Cianchi, F., Giommoni, E., Magnelli, L. and Papucci, L., 2019. Update on gastric cancer treatments and gene therapies. Cancer and Metastasis Reviews, 1-12. - Danciu, C., Muntean, D., Alexa, E., Farcas, C., Oprean, C., Zupko, I., Bor, A., Minda, D., Proks, M., Buda, V., Hancianu, M., Cioanca, O., Soica, C., Popescu, S. and Dehelean, C., 2019. Phytochemical characterization and evaluation of the antimicrobial, antiproliferative and pro-apoptotic potential of Ephedra alata Decne. hydroalcoholic extract against the MCF-7 breast cancer cell line. Molecules, 24(13): 1-15. - Darvishi, M., Esmaeili, S., Dehghan-Nayeri, N., Mashati, P. and Gharehbaghian, A., 2017. Anticancer effect and enhancement of therapeutic potential of vincristine by extract from aerial parts of Juniperus excelsa on pre-B acute lymphoblastic leukemia cell lines. American Journal of Cancer Research; 7(5): 1016-1036. - Dudonne, S., Vitrac, X., Coutie RE, P., Woillez, M. and Merillon, J., 2009. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. Journal of Agricultural and Food Chemistry, 57: 1768-1774. - Fukushima, K., 2004. Bioctivity of Ephedra: Integrating cytotoxicity assessment with real-time biosensnsing. Thesis submitted to the Faculty of the Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirements for the degree of Master of Science. - Ghasemi Gerami, K., 2009. Investigation of biological anti-cancer and antibacterial properties of some nanometer materials and hallucomplexes of intermediate elements. Ph.D. thesis, Department of Biology, Faculty of Science, Mohaghegh Ardabili University, Ministry Science Research and Technology, Ardabil. - Ghazanfari, T., Yaraee, R., Shams, J., Rahmati, B., Radjabian, T. and Hakimzadeh, H., 2013. Cytotoxic effect of four herbal medicines on gastric cancer (AGS) cell line. Food and Agricultural Immunology, 24(1): 1-7. - Ghiasvand, M., Makhdoumi, A., M. Matin, M. and Vaezi, J., 2019. Exploring the bioactive compounds from endophytic bacteria of a medicinal plant: Ephedra foliata (Ephedrales: Ephedraceae). Oriental Pharmacy and Experimental Medicine, 20(1): 1-10. - Hanachi, P., Salehizadeh, Sh., Kiarostami, K. and Ramezani, R., 2019. Investigation of Antioxidant properties of Ocimum basilicum and Impatiens walleriana and their cytotoxic effect on the gastric cancer AGS cell line. Journal of Cell & Tissue, 9(4): 378-388. - Hasannia, M., Ariaii, P. and Fattahi, E., 2016. The effect of extraction methods on phenolic and tocophorol content and antioxidant properties of dill extracts (Anethum graveolens). Iranian Journal of Food Science and Technology, 13(57): 109-119. - Ilic, M. and Ilic, I., 2022. Epidemiology of stomach cancer.Word Journal of Gastroenterology, 28(12): 1187-1203. - Ismail, S., Adwan, G. and jarrar, N., 2020. Evaluation of antimicrobial and genotoxic activity of Ephedra foeminea ethanolic and aqueous extracts on Escherichia coli. Jordan Journal of Biological Sciences, 13(1): 123-126. - Kazemi, N. and Beigi Shahrestani, S., 2018. Effect of saffron extract on expression of bax and Bcl-2 genes in Gastric Adenocarcinoma Cell Line (AGS). Gene cell Tissue, 5(3): e63608. - Kmail, A., Lyoussi, B., Zaid, H. and Saad, B., 2015. In vitro assessments of cytotoxic and cytostatic - Lee, M., Cheng, W., Che, C. and Hsieh, P., 2000. Cytotoxicity assessment of Ma-huang (Ephedra) under different conditions of preparation. Toxicological Sciences, 56: 424-430. - Maddah, S.M., Moraghbi, F. and Sarhadi, S., 2022. Comparison of cytotoxic properties of essential oil and extract of Juniperus excelsa branches of Alborz region on lung cancer cell line A549. Cellular and Molecular Research, 35(4): 655-668. - Maddah, S.M., Mostafavi, G., Amin Malek, M., Anbarestani, M., Sharif, Y. and Mir Hassani, Z., 2021. Combined application of cisplatin and salicylic acid suppresses cell growth and promotes apoptosis in human lung cancer cell lines. Biologia, 77: 215-223. - Majidi Gharenaz, N., Movahedin, M. and Mazaheri, Z., 2015. Evaluating the development and the expression of Bax, Bcl-2, and ErbB4 genes following vitrification of eight cell and blastocyst embryos. Journal of Fasa University of Medical Sciences, 4(4): 427-435. - Mashhadi, F., Ghorbani Nohooji, M. and Yaraee, R., 2021. Effects of essential oils of Origanum vulgare L. and Origanum majorana L. on cancer cells line BCL-1 and immune system cells. Iranian Journal of Medicinal and Aromatic Plants Research, 37(5): 781-794. - Mellado, M., Soto, M., Madrid, A., Montenegro, I., Jara-Gutiérrez, C., Villena, J., Werner, E., Godoy, P. and Aguilar, L., 2019. In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts. BMC Complementary and Alternative Medicine, 19(53): 1-10. - Mighri, H.A., Akrout, A., Bennour, N., Eljeni, H., Zammouri, T. and Neffati, M., 2019. LC/MS method development for the determination of the phenolic compounds of Tunisian Ephedra alata hydro-methanolic extract and its fractions and evaluation of their antioxidant activities. South African Journal of Botany, 124: 102-110. - Mofid Bojnourdi, M., Aqdasi, M., Mianabadi, M. and Nadaf, M., 2016. A Comparative study on some secondary metabolites of male and female stems of Ephedra major Host. Iranian Journal of Medicinal and Aromatic Plants Research, 32(2): 290-300. - Murphy, N.R. and Hellwig, R.J., 1996. Improved nucleic acid organic extraction through use of a unique gel barrier material. BioTechniques, 21: 934-939. - Naderi, M., Cherati, M. R., Mohammadian, A., Bidhendy, M.B., Ghiasvand, S., Marzouni, H.Z. and Javidi, M.A., 2020. Hypericin induces apoptosis in the AGS cell line with no significant effect on normal cells. Iranian Journal of Pharmaceutical Research, 19(3): 349-357. - Noroozi, S., Ahmadi, R. and Iranshahi, M., 2020. The apoptotic effects of tolmetin on human gastric cancer cells. Journal of Tehran University of Medical Sciences (Payavard Salamat), 14(2): 144-151. - Parsa, N., 2012. Molecular and cellular basis of human cancer. Journal of Cell & Tissue. 2(4): 365-376. - Sitarz, R., Skierucha, M., Mielko, J., Offerhaus, G., Maciejewski, R. and Polkowski, W.P., 2018. Gastric cancer: epidemiology, prevention, classification, and treatment. Cancer Management and Research, 10: 239-248. - Soumaya, B., Yosra, E., Rim, M., Dakhlaoui, S., Sawsen, S., Sarra, B., Kamel, M., Wissem, A., Isoda, H. and Wided, M., 2020. Preliminary phytochemical analysis, antioxidant, anti-inflammatory and anticancer activities of two Tunisian Ephedra species: Ephedra alata and Ephedra fragilis. South African Journal of Botany, 135: 1-8. - Sotohian, F., Sharifi, S. and Ranjbaran, M., 2004. The effects of environmental pollution of Robat Karim manganese mine. The First National Conference on Strategies for Achieving Sustainable Development In The Agricultural Sectors of Natural Resources and The Environment, Tehran Iran, 10 March: 131-137. - Torabzadeh Khorasani, P., Panahi, P., Salkbar, A. and Mokhtari, A., 2010. Investigation of antibacterial effects of aqueous, alcoholic and acetic extracts of Goat Beard (Ephedra major Host.) on standard strains of Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Escherichia coli. Comparative Biopathology of Iran, 4: 91-98. - Xu, A.G., Li, S.G., Liu, J.H. and Gan, A.H., 2001. Function of apoptosis and expression of the proteins Bcl-2, p53 and C-myc in the development of gastric cancer. World Journal of Gastroenterology, 7(3): 403-406. | ||
|
آمار تعداد مشاهده مقاله: 321 تعداد دریافت فایل اصل مقاله: 285 |
||