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تأثیر تیمار پرایمینگ بذر بر تحمل به تنش شوری گیاه فلوس (Cassia fistula L.) در مرحله جوانه‌زنی و رشد گیاه‌چه با استفاده از تحلیل تصویر دیجیتال | ||
| علوم و فناوری بذر ایران | ||
| مقاله 2، دوره 11، شماره 4 - شماره پیاپی 28، آذر 1401، صفحه 17-34 اصل مقاله (423.52 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2022.358170.1426 | ||
| نویسندگان | ||
| اسماعیل ابراهیمی1؛ سید امیر موسوی* 2؛ سید عطا اله سیادت3؛ نوراله معلمی4؛ محمد صبائیان5 | ||
| 1گروه مهندسی تولید و ژنتیک گیاهی،دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران | ||
| 2عضو هیأت علمی گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان. | ||
| 3گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، خوزستان، ایران | ||
| 4دانشگاه شهید چمران اهواز | ||
| 5گروه فیزیک، دانشگاه شهید چمران اهواز | ||
| چکیده | ||
| جوانه‌زنی بذر یکی از مهم‌ترین و اساسی‌ترین مراحل رشد و نمو گیاهان می‌باشد، به‌طوری‌که اهمیت و اثر بسزایی در سایر مراحل رشد آن دارد. فلوس، گیاهی از خانواده لگومینوزه و دارای خواص داروئی فراوان است. تکثیر فلوس به‌وسیله بذر انجام می‌شود، اما هنوز مطالعه و گزارشی در خصوص میزان تحمل این بذر به تنش شوری در کشور ارائه نشده است. دو آزمایش فاکتوریل در قالب طرح کامل تصادفی در آزمایشگاه علوم و تکنولوژی بذر دانشگاه علوم کشاورزی و منابع طبیعی خوزستان انجام شد. در آزمایش اول، اثر تیمار هورمون پرایمینگ با جیبرلین، هالوپرامینگ با نیترات پتاسیم و هیدروپرایمینگ بر جوانه‌زنی بذر فلوس بررسی شد. نتایج بدست آمده از این آزمایش نشان داد که بیشترین بنیه بذر در تیمار 50 میلی گرم در لیتر جیبرلین و مدت 12 ساعت پرایمینگ ایجاد شد. پس از انتخاب بهترین تیمار (جیبرلین 50 میلی‌گرم در لیتر به مدت 12 ساعت)، ویژگی‌های جوانه‌زنی بذرهای پرایم شده در شرایط تنش شوری (صفر، 4، 8 ، 14، 16 و 20 dS/m) با بذرهای پرایم نشده مقایسه شدند. نتایج بدست آمده نشان داد که تیمار نتایج نشان داد که میزان تحمل به تنش شوری فلوس در مرحله جوانه‌زنی بطور قابل توجهی با پرایمینگ بذر افزایش یافت. بیشترین بنیه بذر در تیمار پرایمینگ و عدم وجود تنش شوری به میزان (89/337) مشاهده شد در حالی که کمترین میزان بنیه بذر تیمار پرایم شده در تیمار 20 dS/m مشاهده گردید. بذرهای پرایم نشده قادر به تحمل تنش شوری فراتر از 4 dS/m نبودند. | ||
| کلیدواژه‌ها | ||
| بنیه؛ تنش؛ شاخص جوانه زنی | ||
| عنوان مقاله [English] | ||
| Effect of seed priming on salinity tolerance of (Cassia fistula L.) at seed germination and seedling growth stages using digital image analysis | ||
| نویسندگان [English] | ||
| Esmaeil Ebrahimi1؛ Seyed Amir Moosavi2؛ seyed ata Siadat3؛ Nooralah Moallemi4؛ Mohammad Sabaeian5 | ||
| 1Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan | ||
| 2Department of Plant production of Genetics, Khuzestan University of Agricultural Sciences and Natural Resources. | ||
| 3Department of Agronomy and Plant Breeding, Faculty of Agriculture , Khouzestan agricultural and natural resources university, khouzestan, Iran | ||
| 4Shahid Chamran University of Ahvaz | ||
| 5Shahid Chamran University of Ahvaz | ||
| چکیده [English] | ||
| Seed germination is one of the most important and basic stages of plant growth and development so it has great importance and effect on other stages of its growth. Cassia is a plant from the legume family and has many medicinal properties. Cassia propagation is done by seeds, but no study and report on the tolerance of these seeds to salinity stress in the country have been presented yet. Two factorial experiments were performed in a completely randomized design at the Seed Technology Laboratory of Agricultural Sciences and Natural Resources University of Khuzestan. In the first experiment, the effect of hormone priming treatment with gibberellin, halopriming with potassium nitrate, and hydro priming on Cassia seed germination was investigated. The results of this experiment showed that the highest seed vigor was obtained in the treatment of 50 mg/l gibberellin for 12 hours of priming. After selecting the best treatment (gibberellin 50 mg/l for 12 hours), germination characteristics of primed seeds compared with no primed seeds under salinity stress (0, 4, 8, 14, 16, and 20 dS/m). The results showed that seed tolerance to salinity stress at the germination stage increased significantly with seed priming. The highest seed vigor was observed in priming treatment at no salinity stress condition (339.89) while the lowest seed vigor of primed treatment was observed in 20 dS/m. Unprimed seeds could not withstand salinity stress beyond 4 dS/m. | ||
| کلیدواژه‌ها [English] | ||
| Vigor, Stress, Germination index | ||
| مراجع | ||
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