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تاثیر زوال بذر بر صفات فیزیولوژیک و آنزیم‌های آنتی‌اکسیدانی و اس-نیتروزیلاسیون پروتئین‌ها در دورقم کلزا (Brassica napus L.) | ||
| علوم و فناوری بذر ایران | ||
| مقاله 2، دوره 12، شماره 1 - شماره پیاپی 29، اردیبهشت 1402، صفحه 15-28 اصل مقاله (1.97 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2020.342885.1343 | ||
| نویسندگان | ||
| مریم زمانیان1؛ رضا توکل افشاری* 2؛ جعفر نباتی3؛ علیرضا سیفی4 | ||
| 1گروه اگروتکنولوژی/ دانشکده کشاورزی / دانشگاه فردوسی مشهد | ||
| 2استاد | ||
| 3استادیار پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد | ||
| 4استادیار گروه بیوتکنولوژی وبه نژادی گیاهی، دانشگاه فردوسی مشهد | ||
| چکیده | ||
| این مطالعه به صورت آزمایش فاکتوریل بر پایه طرح کاملاً تصادفی با دو عامل رقم (مودنا و اوکاپی) ، سطح پیری (صفر و 24 و 72 ساعت در دمای 40 درجه سانتی‌گراد و رطوبت اشباع) و چهار تکرار برای ارزیابی سرعت و درصد جوانه‌زنی (GP)، شاخص بنیه (SV)، هدایت‌الکتریکی (EC)، فعالیت کاتالاز(CAT)، آسکوربات‌پراکسیداز (APX)، گلوتاتیون‌ردوکتاز (GR) و اس-نیتروزوتیول (SNO) در سال 1399-1398 در دانشگاه فردوسی مشهد انجام شد. تیمارهای پیری تسریع شده (AA) باعث کاهش معنی‌دار کلیه صفات (به جز EC) در بذرهای کلزا شدند. رقم مودنا در صفات جوانه‌زنی قوی‌تر و رقم اوکاپی در فعالیت‌های بیوشیمیایی برتر بود که این را می‌توان با تفاوت ژنتیکی ارقام توجیه کرد. بر اساس نتایج، در تیمارهای 72 ساعت اکاپی نسبت به شاهد، 86% GP، 88% CAT، %89 APX و 36% SNOها کاهش یافته است. هم‌چنین، همبستگی مثبتی بین SNOها و آنزیم‌های آنتی‌اکسیدانی وجود داشت؛ به عنوان نتیجه‌گیری، نیتریک اکسید (NO)به طور غیرمستقیم و به وسیله SNOها بر آنزیم‌های آنتی-اکسیدانی در بذرهای پیرشده کلزا تاثیرگذار است. پیری بذر با کاهش فعالیت آنزیم‌های آنتی‌اکسیدانی و NO و در نتیجه افزایش سرعت مرگ‌و‌میر سلول‌ها، فیزیولوژی سلول را هدف قرار می‌دهد. | ||
| کلیدواژه‌ها | ||
| آسکوربات پراکسیداز؛ پیری؛ کاتالاز؛ گلوتاتیون ردوکتاز؛ نیتریک اکساید | ||
| عنوان مقاله [English] | ||
| Effects of seed deterioration on physiological traits, antioxidant enzymes and S-nitrosylation of proteins in two cultivars of canola (Brassica napus L.) | ||
| نویسندگان [English] | ||
| Maryam Zamanian1؛ Reza Tavakkol afshari2؛ Jafar Nabati3؛ Alireza Seifi4 | ||
| 1Department Agrotechnology/ Agriculture Faculy/ Ferdowsi university of Mashhad | ||
| 2Ferdowsi Univ. Mashhad | ||
| 3Assistant Professor of Crop Physiology, Research Center for Plant Sciences, Ferdowsi University of Mashhad | ||
| 4Assistant Professor, Department of Crop Biotechnology and Breeding, Ferdowsi University of Mashhad | ||
| چکیده [English] | ||
| This study was conducted as a factorial experiments based on a completely randomized design with two factors cultivars (Modena and Okapi), aging level (0, 24 and 72 hours at temperature of 40˚c and relative humidity of saturated) and four replications to evaluate germination rate and percentage (GP), vigor index (SV), electrical conductivity (EC), catalase (CAT), ascorbate peroxides (APX), glutathione reductase (GR) and S-nitrosothiol (SNO) at the Ferdowsi University of Mashhad, 2019-20. Accelerated Aging (AA) treatments made significant decline in germination indices and biochemical characteristics (except EC) of canola seeds. Also, Modena cultivar was stronger in germination traits while Okapi cultivar was better in biochemical activities, which can be explained by difference in the primary genetic of the cultivars. According to the results, in the treatments of 72 hours of AA compared to the control, GP has decreased by 86%, CAT 88%, APX 89% and SNOs 36%. On the top of that, there was a positive correlation between SNOs and enzymes. In conclusion, Nitric oxide (NO) is likely to indirectly affect the antioxidant enzymes by SNOs in aged canola seeds. Seed aging targets the cell physiology by reducing the activities of antioxidant enzymes and NO and therefore increasing the rate of cell mortality. | ||
| کلیدواژه‌ها [English] | ||
| Aging, Ascorbate peroxidase, Catalase, Glutathione reductase, Nitric oxide | ||
| مراجع | ||
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Abdul-Baki, A.A., and J.D. Anderson. 1973. Vigor determination in soybean by multiple criteria. Crop Sci.13: 630-633. Afrosheh, R., H.R. Balouchi, M. Movahedi Dehnavi, and M.H. Gharineh. 2017. The effects of salicylic acid and seed deterioration on germination indices and antioxidant enzymes changes of Carthamus tinctorius L. cv. Soffeh seed. Iranian J. Seed Sci. Technol. 7(1): 53-64. (In Persian, with English Abstract) Anonymous. 2018. FAOSTAT, food and agriculture organization of the United Nations. Arc, E., Galland, M., Cueff, G., Godin, B., Lounifi, I., Job, D and Rajjou, L. 2011. Reboot the system thanks to protein post-translational modifications and proteome diversity: how quiescent seeds restart their metabolism to prepare seedling establishment. Proteomics 1: 1606–1618. Arc, E., Galland, M., Godin, B., Cueef. G and Rajjou, L. 2013. Nitric oxide implication in the control of seed dormancy and germination. Front. Plant Sci. 4(346): 346. Bai, X., Yang, L., Tian, M., Chen, J., Shi, J., Yang, Y and Hu, X. 2011. Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation. J. PLoS ONE. 6(6): e20714. Balouchi, H and Ostadian Bidgoly, R. 2016. Effect of seed deterioration on germination and antioxidant enzymes activity of oil flax (Linum usitatissimum L.) Red Bazrak genotype. Iranian J. Plant Process and Function. 7(23): 205-218. (In Persian, with English Abstract) Balouchi, H.R. and Ostadian Bidgoly, R. 2017. Effect of Seed Deterioration on Physiological and Biochemical Traits of Oil Flax (Linum usitatissimum L. Norman var.) Seed. Iranian J. Plant Prod. (Sci. J. Agric.). 40(2): 37-53. (In Persian, with English Abstract) Balouchi, H., Bagheri, F., kayed-nezami, R., Movahedi-Dehnavi, M and Yadavi. A. 2014. Effect of seed aging on germination and seedling growth indices in three cultivars of Brassica napus L. Iranian J. Plant Res. 26: 397-411. (In Persian) Barreto, L. C and Garcia, QG. 2017. Accelerated ageing and subsequent imbibition affect seed viability and the efficiency of antioxidant system in macaw palm seeds. Acta. Physiol. Planta. 39(3): 1-8. Begara-Morales, J. C., Chaki, M., Valderrama, R., Mata-Pérez, C., Padilla, M. N and Barroso, J. B. 2019. The function of S-nitrosothiols during abiotic stress in plant. J. Exp. Bot.70(17): 4429–4439. Bethke, P. C., Badger, M. R., Jones, R .L. 2004. Apoplastic Synthesis of Nitric Oxide by Plant Tissues. The Plant Cell. 16(2): 332-41. Bethke, P. C., Libourel, I. G., Vitecek, J and Jones, R. L. 2011. Nitric Oxide Methods in Seed Biology. Methods in Mol. Biol.773: 385-400. Cao, D., Chen, S., Huang, Y., Qin, Y and Ruan, G. 2019. Effects of artificial aging on physiological characteristics of rice seeds with different dormancy characteristics. Agric. Biotechnol. 8(1): 52-56. Corpas, F. J and Barroso, J. B. 2015. Nitric oxide from a “green” perspective. Function of nitric oxide under physiological and stress conditions. 45: 15-19. PLoS ONE 6(6): e20714. doi:10.1371/journal.pone.0020714 Demirkaya, M., Dietz, K. J and Sivitepe, S. 2010. Changes in antioxidant enzymes during ageing of onion seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 38(1):49-52. Demirkaya. 2013. Relationships between antioxidant enzymes and physiological variations occur during ageing of pepper seeds. Hortic. Environ. Biotechnol. 54(2): 97-102. Elias, S. G and Copeland, O. 1994. The effect of storage conditions on canola (Brassica napus L.). J. Seed Technol. 18: 21-29. Ellis, R. H., Hong, T. D and Roberts, E. H. 1985. Handbook of seed technologies for gene banks.International Board for Plant Genetic Resources, Rome, Italy. Goel, A., Goel, A. K., AK and Sheoran, I. S. 2003. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. J. Plant Physiol. 160(9): 1093-100. Hampton, J. G and Tekrony, D. M. 1995. Handbook of vigour test methods. ISTA, Zurich, Switzerland. He, Y., Xue, H., Li, Y and Wang, X. 2018. Nitric oxide alleviates cell death through protein S-nitrosylation and transcriptional regulation during the ageing of elm seeds. J. Exp. Bot. 69(21): 5141–5155. Hell, R., Arnold, G. J., Durner, J and Lindermayr, C. 2016. ROS-Mediated Inhibition of S-nitrosoglutathione Reductase Contributes to the Activation of Anti-oxidative Mechanisms. Front. Plant Sci. 7(126). Hess, D.T., Matsumoto, A., Kim, S. O., Marshall, H. E., Stamler, J. S . 2005. Protein S-nitrosylation: purview and parameters. Nature Reviews Mol. Cell Biol. 6(2): 150-66. ISTA (International Seed Testing Association). 2015. International Rules for Seed Testing ISTA, Bassersdorf, Switzerland. Jain, P., Toerne, C. V., Lindermayr, C and Bhatla, S. C. 2018. S-nitrosylation/denitrosylation as a regulatory mechanism of salt stress sensing in sunflower seedlings. Physiologia Plantarum. 162(1): 49-72. Janmohammadi, M., Fallahnezhad,Y., Golshan, M and Mohammadi, H. 2008. Controlled ageing for storability assessment and predicting seedling early growth of canola cultivars (Brassica napus L.). ARPN J. Agric. Biol. Sci. 3(5): 22-26. Kong, L., Huo, H and Mao, P. 2015. Antioxidant response and related gene expression in aged oat seed. Front. Plant Sci. 6(158): 158. Lee, DH and Lee, CB. 2000. Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. Plant Sci. 159(1): 75-85. Lehner, A., Mamadou, N., Poels, P., Coˆme, D., Bailly, C and Corbineau, F. 2008. Changes in soluble carbohydrates, lipid peroxidation and antioxidantenzyme activities in the embryo during ageing in wheat grains. J. Cereal Sci. 47(3): 555-565. Ma, Z., Marsolais, F., Bykova, N. V and Igmaberdiev, A. U. 2016. Nitric oxide and reactive oxygen species mediate Metabolic changes in barley seed embryo during germination. Front. Plant Sci. 7(138): 1-13. Maguire, J. D. 1962. Speed of germination in selection and evaluation for seedling vigor. Crop Sci. 2: 176-177. Mehravar, M., Sateai, A., Hamidi, A., Ahmadi, M and Salehi, M. 2014. Accelerated aging effect on lipid peroxidation and antioxidant enzymes activity of two soybean cultivars. Iranian J. Seed Sci. Technol. 13(1): 17-30. (In Persian, with English Abstract) Mohammadzadeh, A., S.Asadi, N. Majnoun Hosseini, H. Moghadam and M. Jamali. 2017. Effects of artificial seed ageing on germination indices, seedling establishment and yield of two red kidney bean (Phaseolus vulgaris L.) cultivars. Iranian J. Seed Sci. Technol. 7(2): 75-94. (In Persian, with English Abstract) Omidi, H., Tahmasebi, Z., Naghdi Badi, H. A., Torabi, H and Miransari, M. 2010. Fatty acid composition of canola (Brassica napus L.), as affected by agronomical, genotypic and environmental parameters. C. R. Biol. 333(3): 248-54.Oskouei, B., Divsalar, M., Yari, L and zareyan. A. 2012. Effect of packaging type on seed vigor of three varieties of canola stored in Qom. Iranian J. Seed Sci. Technol. 2(1): 13-24. (In Persian) Paravar, A., Maleki Farahani, S and Gholami, S. 2019. Effect of seed deterioration on germination, biochemical and enzamatic activity of buckwheat. Iranian J. Agroecol. 15(2): 49-57. (In Persian, with English Abstract)Sahu, B., Sahu, A. K., Thomas, V and Naithani, S. C. 2017. Reactive oxygen species, lipid peroxidation, protein oxidation and antioxidative enzymes in dehydrating Karanj (Pongamia pinnata) seeds during storage. South Afr. J. Bot. 112: 383-390. Sanchouli, A., Ramezanzadeh, N., Ghaderifar, F., Sadeghipour, H. R and Soltani, A. 2011. Investigating the effect of deterioration on germination and activity of catalase and peroxidase enzymes in cotton (Gossypium Hirsutum L) seeds. 1st National Conference on Strategies to Achieve Sustainable . (In Persian) Scandalios, J.G., Guan, L. and Polidoros, A.N. 1997. Catalases in plants: gene structure, properties, regulation and expression., J.G. (Ed.) Oxidative stress and the molecular biology of antioxidant defenses. New York, Cold Spring Harbor Lab Press. 34: 343–406. Sen, SH. 2010. S-Nitrosylation process acts as a regulatory switch for seed germination in wheat. Am. J. Plant Physiol. 5(3): 122-132. Shakirova, F.M. and Sahabutdinova, D.R. 2003. Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Sci. 164: 317-322. Stamler, G.S., Singel, G. S and Loscalzo, J. 1993. Biochemistry of Nitric Oxide and its Redox-Activated forms. Science. 258(5090): 1898-902. Stamler, J. S., Lamas, S and Fang, F. C. 2001. Nitrosylation. the prototypic redox-based signaling mechanism. Cell. 106(6): 675-83. Tavakol Afshari, R., Chasemi, F., Majnoun Hosseini, N., Alizadeh, H and Bihamta, M. R. 2007. Some effects of seed aging on germination characteristic and activities of catalase and peroxidase antioxidant enzymes in barley genotypes (Hordeum vulgare). Iranian J. Agric. Sci. 38(1): 337-346. (In Persian) Velikova, V., Yordanov, I and Edreva, A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Sci. 151: 59-66. Verma, S., U, Verma and Tomer, R. 2003. Studies on seed quality parameters in deteriorating seeds in Brassica (Brassica campestris). Seed Sci. Technol. 31(2): 389-396. Viteceka, J., Reinohla, V and Jones, R. L. 2007. Measuring NO Production by plant tissues and suspension cultured cells. Mol. Plant 1(2): 270-284. Wink, D. A and Mitchell, J.B. 1998. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Curr. Topics in Cell. Regul. 25(4-5): 434-456. Yamaguchi, K., Yamaguchi, H. and Nishimura, M. 1995. A novel isoenzyme of ascorbate peroxidase localized on glyoxysomal and leaf peroxisomal membranes in pumpkin. Plant Cell Physiol. 36(6): 1157-1162. Yang, H., Mu, J., Chen, L., Feng, J., Hu, J., Li, L., Zhou, J.M. and Zuo, J. 2015. S-nitrosylation positively regulates ascorbate peroxidase activity during plant stress responses. Plant Physiol. 167(4): 1604–1615. Yin, G., Xin, X., Song, C., Chen, X., Zhang, J., Wu, S., Li, R., Liu, X and Lu, X. 2014. Activity levels and expression of antioxidant enzymes in the ascorbateeglutathione cycle in artificially aged rice seed. Plant Physiol. Biochemist. 80: 1-9. Yin, X., He, D., Gupta, R and Yang. P. 2015. Physiological and proteomic analyses on artificially aged Brassica napus seed. Front. Plant Sci. 6(112): 1-11.
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