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Ahangarzadeh, M., Houshmand, H., Kakoolaki, S., Sepahdari, A., Ghorbanpoor, M., Ajdari, A., Nazemroaya, S., Zabayeh Najafabadi, M., Torfi Mozanzadeh, M., Sadat Sadr, A.. (1401). Efficiency of monovalent Vibrio alginolyticus formaldehyde-killed vaccine on the immune responses and protection of Asian seabass (Lates calcarifer) juveniles against Vibriosis. سامانه مدیریت نشریات علمی, 21(6), 1367-1382. doi: 10.22092/ijfs.2022.128211
M. Ahangarzadeh; H. Houshmand; S. Kakoolaki; A. Sepahdari; M. Ghorbanpoor; A. Ajdari; S. Nazemroaya; M. Zabayeh Najafabadi; M. Torfi Mozanzadeh; A. Sadat Sadr. "Efficiency of monovalent Vibrio alginolyticus formaldehyde-killed vaccine on the immune responses and protection of Asian seabass (Lates calcarifer) juveniles against Vibriosis". سامانه مدیریت نشریات علمی, 21, 6, 1401, 1367-1382. doi: 10.22092/ijfs.2022.128211
Ahangarzadeh, M., Houshmand, H., Kakoolaki, S., Sepahdari, A., Ghorbanpoor, M., Ajdari, A., Nazemroaya, S., Zabayeh Najafabadi, M., Torfi Mozanzadeh, M., Sadat Sadr, A.. (1401). 'Efficiency of monovalent Vibrio alginolyticus formaldehyde-killed vaccine on the immune responses and protection of Asian seabass (Lates calcarifer) juveniles against Vibriosis', سامانه مدیریت نشریات علمی, 21(6), pp. 1367-1382. doi: 10.22092/ijfs.2022.128211
Ahangarzadeh, M., Houshmand, H., Kakoolaki, S., Sepahdari, A., Ghorbanpoor, M., Ajdari, A., Nazemroaya, S., Zabayeh Najafabadi, M., Torfi Mozanzadeh, M., Sadat Sadr, A.. Efficiency of monovalent Vibrio alginolyticus formaldehyde-killed vaccine on the immune responses and protection of Asian seabass (Lates calcarifer) juveniles against Vibriosis. سامانه مدیریت نشریات علمی, 1401; 21(6): 1367-1382. doi: 10.22092/ijfs.2022.128211

Efficiency of monovalent Vibrio alginolyticus formaldehyde-killed vaccine on the immune responses and protection of Asian seabass (Lates calcarifer) juveniles against Vibriosis

Iranian Journal of Fisheries Sciences
دوره 21، شماره 6، بهمن 2022، صفحه 1367-1382  XML اصل مقاله (356.09 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2022.128211
نویسندگان
M. Ahangarzadeh1؛ H. Houshmand* 1؛ S. Kakoolaki2؛ A. Sepahdari2؛ M. Ghorbanpoor3؛ A. Ajdari4؛ S. Nazemroaya1؛ M. Zabayeh Najafabadi1؛ M. Torfi Mozanzadeh1؛ A. Sadat Sadr1
1South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Ahvaz, Iran.
2Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Tehran, Iran.
3Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
4Offshore Fisheries Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Chabahar, Iran.
چکیده
Vibrio alginolyticus causes severe health problems in marine fish production each year. Among various therapeutic strategies, vaccination is the most economic, efficient and environmentally-friendly approach against microbial infections. Evaluation of formalin-killed Vibrio alginolyticus vaccine aquired from native isolates was carried out using a total of 200 Asian seabass juveniles (55±6.43 g), which were divided among three groups, including (I) control (non-vaccinated), (II) vaccinated with killed V. alginolyticus and (III) killed vaccine with oral booster. Fish were vaccinated intraperitoneally. Blood samples were taken from fish in each group at three, five, and eight weeks after immunization to assess the antibody levels against V. alginolyticus infection. The efficacy of the killed vaccine was appraised five weeks after the start of the initial vaccination by challenging with twofold LD50 (3.66×108) equivalent of the live suspension of V. alginolyticus through intraperitoneal injection. The results of ELISA showed that there were not any significant differences in antibody response among different groups before vaccination (p>0.05). Also, the mean antibody titer of the group immunized killed-V. alginolyticus with oral booster was significantly higher than the other groups five weeks after the vaccination. The vaccined fish demonstrated higher survival rates than the control with relative percent survival (RPS) of 84.62 and 76.92%, respectively. It is concluded that vaccination could be an effective method to protect farmed Asian sea bass against vibriosis caused by pathogenic V. alginolyticus.
کلیدواژه‌ها
Vibriosis؛ Vibrio alginolyticus؛ Killed vaccine؛ Antibody titer؛ Survival rate
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