1Lab. Techniques Department, College of Health and Medical Techniques -Baghdad, Middle Technical University (MTU), Iraq.
2Radiological Techniques Department, College of Health and Medical Techniques-Baghdad, Middle Technical University (MTU), Iraq.
Helicobacter pylori is considered one of the difficult types of bacteria that are difficult to treat with the currently used antibiotics of amoxicillin, erythromycin and metronidazole, which have proven ineffective against these bacteria. In our current work, modern technology was used to treat these bacteria by converting the aforementioned antibiotics to their Nano state by using the lyophilization method and diagnosed using scanning electron microscopy (SEM).
A mixture of the three Nano-antibiotics was prepared together in a Nano-Medicine and was used to treat H. pylori bacteria in cultures to determine their effectiveness on these bacteria, and the results were highly effective in inhibiting these bacteria at the lowest concentration (OD=0.042). And with the highest concentration (OD = 0.038) compared with the three micro-antibiotics individually, where the OD values of each of amoxicillin, azithromycin and metronidazole, respectively were 0.523, 0.521, 0.453, and the OD values of the three Nano-antibiotics were given for each of the Nano-amoxicillin, Nano-azithromycin, and Nano- metronidazole, respectively, 0.386, 0.258 , 0.167 It can be observed that the percentage of inhibition in each of the Nano-antibiotics is higher than the inhibition in the micro-antibiotics, which happens that the Nano-Medicine had much higher inhibition than each of the three micro- and Nano-antibiotics alike.
The safety of using Nano-antibiotics in the prepared Medicine was confirmed by the use of electrochemical technology by cyclic voltammetric device to identify the electrochemical properties through oxidation and reduction in the blood media. The results of the study showed the appearance of reduction peaks only and the absence of any oxidation peaks in the prepared kit, as well as for each of the three nano-antibiotics separately, and the result was that they are all non-oxidants. So it can be used safely as it is a good antioxidant as a treatment. While the same three micro treatments showed blood oxidation due to the appearance of oxidation peaks for all of them.
The study proved that all H. pylori isolates are resistant to all usable antibiotics. All of the antibiotics in the Nano-Medicine have an anti-bacterial effect.