| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,034 |
| تعداد مقالات | 33,949 |
| تعداد مشاهده مقاله | 45,420,574 |
| تعداد دریافت فایل اصل مقاله | 69,230,942 |
جداسازی و شناسایی قارچ (Boletales: Paxillaceae) Paxillus involutus به‌عنوان همزیست اکتومیکوریزایی صنوبر و تأثیر آن بر تنش خشکی در کبوده (.Populus alba L) | ||
| تحقیقات جنگل و صنوبر ایران | ||
| مقاله 2، دوره 30، شماره 3 - شماره پیاپی 89، آذر 1401، صفحه 211-223 اصل مقاله (401.21 K) | ||
| نوع مقاله: علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijfpr.2022.360008.2068 | ||
| نویسندگان | ||
| نرگس سپاسی1؛ عبدالحسین طاهری* 2؛ سیده معصومه زمانی3؛ مهدی جهانی4؛ محمد ابراهیم فراشیانی3 | ||
| 1دانشجوی دکتری بیماری‌شناسی گیاهی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| 2نویسنده مسئول، دانشیار، گروه بیماری‌شناسی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| 3استادیار، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 4دانشیار، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران | ||
| چکیده | ||
| کم‌آبی و روند افزایش گرما در سال‌‌های اخیر، محدودیت‌‌هایی را برای کشت و پرورش صنوبر ایجاد کرده‌اند. قارچ‌‌های اکتومیکوریز، نقش مؤثری در افزایش تحمل گیاهان در مواجهه با تنش‌‌های آبی، شوری و دفاع در برابر آفات و بیماری‌‌ها دارند. در پژوهش پیش‌رو، جداسازی و شناسایی ریخت‌شناختی و مولکولی قارچ‌‌های اکتومیکوریز از رویشگاه‌‌های صنوبر استان گیلان (شفارود، گیسوم و صفرابسته) انجام گرفت. براساس این اطلاعات، قارچ اکتومیکوریز Paxillus involutus به‌عنوان همزیست صنوبر شناسایی و معرفی شد. از میسلیوم این قارچ برای تلقیح به گیاه‌چه‌‌‌‌های کبوده (.Populus alba L) در شرایط گلخانه و بررسی تأثیر آن بر پارامترهای رشدی گیاه در شرایط تنش خشکی و آبیاری استفاده شد. نتایج نشان داد که برقراری ارتباط همزیستی بین گیاه‌چه‌‌‌‌های کبوده و P. involutus موفقیت‌آمیز است. به‌طوری‌که صفات رشدی شامل وزن تر و خشک ریشه، وزن تر و خشک ساقه، وزن تر برگ، سطح برگ و ارتفاع ساقه در گیاه‌چه‌‌‌‌های تلقیح‌شده با این قارچ همزیست نسبت به گیاه‌چه‌‌‌‌های تیمار شاهد به‌طور معنی‌داری بیشتر بودند (01/0P<). همچنین، P. involutus با کاهش اثرات منفی تنش خشکی بر کبوده سبب افزایش توان تحمل گیاه‌چه‌‌‌‌های میکوریزی شد. براساس این یافته‌ها، وجود سیستم هماهنگ قارچ- گیاه می‌‌تواند نقش مؤثری در بهبود عملکرد درختان کبوده در شرایط تنش خشکی ایفا کند. | ||
| کلیدواژه‌ها | ||
| تلقیح؛ صنوبر؛ گیاه‌چه؛ همزیستی اکتومیکوریزایی | ||
| عنوان مقاله [English] | ||
| Isolation and characterization of Paxillus involutus (Boletales: Paxillaceae) as an ectomycorrhizae of poplar and its effect on drought stress in White poplar (Populus alba L.) | ||
| نویسندگان [English] | ||
| N. Sepasi1؛ A. Taheri2؛ S.M. Zamani3؛ M. Jahani4؛ M.E. Farashiani3 | ||
| 1Ph.D. Student of Plant Pathology, Department of Plant Pathology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran | ||
| 2Corresponding Author, Associate Prof., Department of Plant Pathology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran | ||
| 3Assistant Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| 4Associate Prof., Department of Plant Protection, College of Agriculture, University of Birjand, Birjand, Iran | ||
| چکیده [English] | ||
| In recent years, increasing temperature and water deficiency have caused restrictions on poplar cultivation. Ectomycorrhizal fungi (ECM) play an effective role in increasing plant tolerance against water stress, salinity, and the defense of plants against pests and diseases. In this study, isolation, morphological and molecular identification of ectomycorrhizal fungi of three poplar habitats in Guilan province (Shafaroud, Gisoom and Safra-basteh sites) in Iran was carried out. Based on morphological and molecular data, the ectomycorrhizal fungus Paxillus involutus was identified as poplar ectomycorrhizae. Fungal mycelium was used to inoculate seedlings of Populus alba L. in the greenhouse condition, and the effect of the ectomycorrhizal fungus on the plant growth parameters was measured in water stress and irrigation. The results showed that establishing a symbiotic relationship between poplar seedlings and ectomycorrhizal fungus P. involutus was successful. Moreover, inoculation of the symbiotic fungus positively affected improving the growth characteristics of the inoculated plants. Thus, symbiotic seedlings with ectomycorrhizal fungus were significantly different from the non-mycorrhizal seedlings in plant growth variables including root fresh and dry weight, leaf fresh weight, stem fresh and dry weight, leaf area, and stem height (p<0.01). Also, the use of symbiotic fungus reduced the negative effects of water deficit stress on poplar seedlings and increased the tolerance in the mycorrhizal plants. Based on these findings, a coordinated plant-fungal system plays an effective role in improving the performance of poplar plants under water-stress conditions. | ||
| کلیدواژه‌ها [English] | ||
| Ectomycorrhizal symbiosis, inoculation, Populus, seedling | ||
| مراجع | ||
|
- Assadi, F., Mirzai - Nodushan, H., Modirrahmati, E. and Naderi - Shahab, M.A., 2004. Identification of poplar clones, using morphological markers. Iranian Journal of Forest and Poplar Research, 12(2): 267-300 (In Persian with English summary). - Beaudette, P.C., Chlup, M., Yee, J. and Emery, R.J.N., 2007. Relationships of root conductivity and aquaporin gene expression in Pisum sativum: diurnal patterns and the response to HgCl2 and ABA. Journal of Experimental Botany, 58(6): 1291-1300. - Cortleven, A., Leuendorf, J.E., Frank, M., Pezzetta, D., Bolt, S. and Schmulling, T., 2019. Cytokinin action in response to abiotic and biotic stresses in plants. Plant, Cell and Environment, 42(3): 998-1018. - Danielsen, L. and Polle, A., 2014. Poplar nutrition under drought as affected by ectomycorrhizal colonization. Environmental and Experimental Botany, 108: 89-98. - Du, P., Tu, H., Jiang, J., Cui, B. and Dai, Y., 2020. Molecular identification and biological characteristics of wild Paxillus ammoniavirescens strain. Forest Research, 33(3): 146-155 (In Chinese with English summary). - Fischer, U. and Polle, A., 2010. Populus responses to abiotic stress: 225-246. In: Jansson, S., Bhalerao, R. and Groover, A. (Eds.). Genetics and Genomics of Populus. Springer, New York, 384p. - Gardes, M. and Bruns, T.D., 1993. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2): 113-118. - Gelardi, M., Segneri, G., Ercole, E. and Vizzini, A., 2011. Paxillus involutus f. eburneus f. nov. (Agaricomycetes, Boletales), a molecularly confirmed infraspecific taxon in the P. involutus complex from Italy. Mycosphere, 2(5): 547-554. - Hahn, C., 2000. Is it possible to separate Paxillus involutus and P. rubicundulus by spore measurement only? Zeitschrift für Mykologie, 66(2): 161-171 (In German with English summary). - Hamanishi, E.T., Thomas, B.R. and Campbell, M.M., 2012. Drought induces alterations in the stomatal development program in Populus. Journal of Experimental Botany, 63(13): 4959-4971. - Henrici, A., 2004. A key to Paxillus s.1. in Europe. Field Mycology, 5(3): 87-88 - Jargeat, P., Chaumeton, J.P., Navaud, O., Vizzini, A. and Gryta, H., 2014. The Paxillus involutus (Boletales, Paxillaceae) complex in Europe: Genetic diversity and morphological description of the new species Paxillus cuprinus, typification of P. involutus s.s., and synthesis of species boundaries. Fungal Biology, 118(1): 12-31. - Kirk, P.M., 2019. Microsporidia: Unicellular spore-forming protozoan parasites (version Nov 2015). In: Roskov, Y., Ower, G., Orrell, T., Nicolson, D., Bailly, N., Kirk, P.M., … and Penev, L. (Eds.). Species 2000 & ITIS Catalogue of Life, 2019 Annual Checklist. Naturalis, Leiden, the Netherlands. Digital resource available at: www.catalogueoflife.org/annual-checklist/2019 - Landhäusser, S.M., Mushin, T.M. and Zwiazek, J.J., 2002. The effect of ectomycorrhizae on water relations in aspen (Populus tremuloides) and white spruce (Picea glauca) at low soil temperatures. Canadian Journal of Botany, 80(6): 684-689. - Loewe, A., Einig, W., Shi, L., Dizengremel, P. and Hampp, R., 2000. Mycorrhiza formation and elevated CO2 both increase the capacity for sucrose synthesis in source leaves of spruce and aspen. The New Phytologist, 145(3): 565-574 - Luo, Z.B., Li, K., Jiang, X. and Polle, A. 2009. Ectomycorrhizal fungus (Paxillus involutus) and hydrogels affect performance of Populus euphratica exposed to drought stress. Annals of Forest Science, 66: 106. - Moore, A.E.P. and Peterson, R.L., 1992. Effect of temperature on sclerotium induction in Paxillus involutus. Canadian Journal of Microbiology, 38(11): 1197-1201. - Otgonsuren, B., Rewald, B., Godbold, D.L. and Göransson, H., 2016. Ectomycorrhizal inoculation of Populus nigra modifies the response of absorptive root respiration and root surface enzyme activity to salinity stress. Flora, 224: 123-129. - Sakakibara, S.M., Jones, M.D., Gillespie, M., Hagerman, S.M., Forrest, M.E., Simard, S.W. and Durall, D.M., 2002. A comparison of ectomycorrhiza identification based on morphotyping and PCR-RFLP analysis. Mycological Research, 106(8): 868-878. - Schützendübel, A. and Polle, A., 2002. Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. Journal of Experimental Botany, 53(372): 1351-1365 - Smith, S.E. and Read, D.J., 2008. Mycorrhizal Symbiosis, Third Edition. Academic Press, New York, 800p. - Stefani, F.O.P., Moncalvo, J.M., Séguin, A., Bérubé, J.A. and Hamelin, R.C., 2009. Impact of an 8-year-old transgenic poplar plantation on the ectomycorrhizal fungal community. Applied and Environmental Microbiology, 75(23): 7527-7536. - Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S., 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony Methods. Molecular Biology and Evolution, 28(10): 2731-2739. - Taylor, A.F.S., Martin, F. and Read, D.J., 2000. Fungal diversity in ectomycorrhizal communities of Norway spruce [Picea abies (L.) Karst.] and beech (Fagus sylvatica L.) along north-south transects in Europe: 343-365. In: Schulze, E.D. (Ed.). Carbon and Nitrogen Cycling in European Forest Ecosystems. Ecological Studies, Vol. 142, Springer, Berlin, Heidelberg, 506p. - Tedersoo, L., Bahram, M., Toots, M., Diédhiou, A.G., Henkel, T.W., Kjøller, S., ... and Kõljalg, U., 2012. Towards global patterns in the diversity and community structure of ectomycorrhizal fungi. Molecular Ecology, 21: 4160-4170. - Tedersoo, L., Jairus, T., Horton, B.M., Abarenkov, K., Suvi, T., Saar, I. and Kõljalg, U., 2008. Strong host preference of ectomycorrhizal fungi in a Tasmanian wet sclerophyll forest as revealed by DNA barcoding and taxon-specific primers. New Phytologist, 180(2): 479-490. - Vellinga, E.C., Blanchard, E.P., Kelly, S. and Contu, M., 2012. Paxillus albidulus, P. ammoniavirescens, and P. validus revisited. Mycotaxon, 119: 351-359. - Walting, R. and Hills, A.E., 2005. British Fungus Flora: Agarics and Boleti 1: Boletes and their Allies: Boletaceae: Strobilomycetaceae: Gyroporaceae: Paxillaceae: Coniophoraceae: Gomphidiaceae. Published by Royal Botanic Garden Edinburgh, Edinburgh, 200p. - Wilkinson, S. and Davies W.J., 2010. Drought, ozone, ABA and ethylene: new insights from cell to plant to community. Plant, Cell and Environment, 33(4): 510-525. - Wu, Q.S., Xia, R.X., Zou, Y.N. and Wangi, G.Y., 2007. Osmotic solute responses of mycorrhizal citrus (Poncirus trifoliata) seedlings to drought stress. Acta Physiologiae Plantarum, 29: 543-549. - Wu, Q.S. and Zou, Y.N., 2009. Mycorrhiza has a direct effect on reactive oxygen metabolism of drought-stressed citrus. Plant, Soil and Environment, 55(10): 436-442. - Yousefi, B. and Modir Rahmati, A.R., 2018. Evaluation of growth and yield of black poplar (Populus nigra L.) clones under drought stress period in comparative populetum of Sanandaj. Iranian Journal of Forest and Poplar Research, 26(2): 276-290 (In Persian with English summary). - Zamani, S.M., Farashiani, M.E., Kazerani, F., 2020. The effect of ectomycorrhizal symbiosis on drought tolerance in Populus caspica. Biocontrol in Plant Protection, 8(1): 11-27 (In Persian with English summary). - Zhou, Z., Miwa, M., Matsuda, Y. and Hogetsu, T., 2001. Spatial distribution of the subterranean mycelia and ectomycorrhizae of Suillus grevillei genets. Journal of Plant Research, 114: 179-185. | ||
|
آمار تعداد مشاهده مقاله: 442 تعداد دریافت فایل اصل مقاله: 333 |
||