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Al-Hassani, A. S, Al-Hassani, D. H, Abdul-Hassan, I. A. (1402). PCR-RFLP Analysis of Insulin-Like Growth Factor 2 Gene Polymorphisms in Two Commercial Broiler Chicken Strains (Cobb 500 and Hubbard F-15) and Their Associations with Performance Traits. سامانه مدیریت نشریات علمی, 78(3), 1153-1157. doi: 10.22092/ari.2022.359894.2504
A. S Al-Hassani; D. H Al-Hassani; I. A Abdul-Hassan. "PCR-RFLP Analysis of Insulin-Like Growth Factor 2 Gene Polymorphisms in Two Commercial Broiler Chicken Strains (Cobb 500 and Hubbard F-15) and Their Associations with Performance Traits". سامانه مدیریت نشریات علمی, 78, 3, 1402, 1153-1157. doi: 10.22092/ari.2022.359894.2504
Al-Hassani, A. S, Al-Hassani, D. H, Abdul-Hassan, I. A. (1402). 'PCR-RFLP Analysis of Insulin-Like Growth Factor 2 Gene Polymorphisms in Two Commercial Broiler Chicken Strains (Cobb 500 and Hubbard F-15) and Their Associations with Performance Traits', سامانه مدیریت نشریات علمی, 78(3), pp. 1153-1157. doi: 10.22092/ari.2022.359894.2504
Al-Hassani, A. S, Al-Hassani, D. H, Abdul-Hassan, I. A. PCR-RFLP Analysis of Insulin-Like Growth Factor 2 Gene Polymorphisms in Two Commercial Broiler Chicken Strains (Cobb 500 and Hubbard F-15) and Their Associations with Performance Traits. سامانه مدیریت نشریات علمی, 1402; 78(3): 1153-1157. doi: 10.22092/ari.2022.359894.2504

PCR-RFLP Analysis of Insulin-Like Growth Factor 2 Gene Polymorphisms in Two Commercial Broiler Chicken Strains (Cobb 500 and Hubbard F-15) and Their Associations with Performance Traits

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مقاله 46، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 1153-1157    اصل مقاله (339.74 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359894.2504
نویسندگان
A. S Al-Hassani* 1؛ D. H Al-Hassani1؛ I. A Abdul-Hassan2
1Department of Animal Production, Collage of Agricultural Engineering Science, University of Baghdad, Baghdad, Iraq
2Genetic Engineering and Biotechnology Institute for Postgraduate Study, University of Baghdad, Baghdad, Iraq
چکیده
The present research aimed to study the polymorphisms of the chicken insulin-like growth factor 2 (IGF2) in two commercial broiler breeds (Cobb 500 and Hubbard F-15). In total, 300 avian blood samples were obtained. The genomic DNA was isolated using a fast salt-extraction technique. Moreover, polymerase chain reaction (PCR) was used to amplify 1146 bp fragments of the gene. The amplified fragments were subjected to restriction enzyme digestion using the HinfI endonuclease enzyme, and the digested products were separated on a 2% agarose gel. The findings indicated that there were two alleles T and C for the target locus, with frequencies of 73.3% and 26.7%, respectively. Three distinct genotype variations, TT, TC, and CC, were found, with genotype frequencies of 59.1%, 28.4%, and 12.5%, respectively. A test based on actual and anticipated frequencies of various genotypic variances of the IGF2 gene revealed that the divergence from Hardy-Weinberg equilibrium was not significant (P≤0.01) in commercial broiler breeds (Cobb 500 and Hubbard F-15) chickens. In addition, it was found that birds with genotype TC had a greater body mass at 8 weeks of age, compared to those with genotypes TT and CC. It was determined that the IGF2 gene exhibited a significant degree of variability and might be regarded as a possible genetic marker in selection and breeding programs for poultry.
کلیدواژه‌ها
IGF2؛ Polymorphism؛ PCR؛ Chicken
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مراجع
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