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Asad Salman, R, Khudhur Jameel, S, Mahdi Shakir, S. (1402). Evaluation of the Effects of Probiotics and Prebiotics on the Salmonella typhi Infections. سامانه مدیریت نشریات علمی, 78(3), 1115-1130. doi: 10.22092/ari.2022.359937.2519
R Asad Salman; S Khudhur Jameel; S Mahdi Shakir. "Evaluation of the Effects of Probiotics and Prebiotics on the Salmonella typhi Infections". سامانه مدیریت نشریات علمی, 78, 3, 1402, 1115-1130. doi: 10.22092/ari.2022.359937.2519
Asad Salman, R, Khudhur Jameel, S, Mahdi Shakir, S. (1402). 'Evaluation of the Effects of Probiotics and Prebiotics on the Salmonella typhi Infections', سامانه مدیریت نشریات علمی, 78(3), pp. 1115-1130. doi: 10.22092/ari.2022.359937.2519
Asad Salman, R, Khudhur Jameel, S, Mahdi Shakir, S. Evaluation of the Effects of Probiotics and Prebiotics on the Salmonella typhi Infections. سامانه مدیریت نشریات علمی, 1402; 78(3): 1115-1130. doi: 10.22092/ari.2022.359937.2519

Evaluation of the Effects of Probiotics and Prebiotics on the Salmonella typhi Infections

Archives of Razi Institute
مقاله 42، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 1115-1130    اصل مقاله (1.24 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359937.2519
نویسندگان
R Asad Salman* 1؛ S Khudhur Jameel2؛ S Mahdi Shakir3
1Medical City, National Center of Teaching Laboratories, Baghdad, Iraq
2Medical Microbiology Department, Medical College, Al-Iraqia University, Baghdad, Iraq
3Medical City Department, National Center for Educational Laboratories, Baghdad, Iraq
چکیده
Typhoid fever is one of the most commonly disseminated diseases and is considered to be linked to poor sanitation. It is responsible for 2-5% of all deaths, and its causative agent is Salmonella typhi. The current study aimed to investigate the antibacterial activity of prebiotics (inulin and starch) and probiotics against multidrug resistance of S. typhi bacterial isolates. Determination of the inhibitory effect of probiotics and prebiotics against S. typhi isolates was performed by agar well diffusion method and minimal inhibitory concentration. Body samples of all eligible patients were collected and cultured. Finally, 50 (25%) out of the total cultured samples were S. Typhi bacteria isolated from different samples. The bacteria were mainly found in blood, followed by stool and fluid (74%, 24%, and 2%, respectively). On differential medium, xylose lysine deoxycholate agar, the colonies appear red with black centers, while on MacConkey agar, the colonies appear smooth, pale, transparent, colorless, and raised. Regarding the inhibition zone values of bacteriocins of Lactobacillus from Yogurt against S. typhi in plate, significant differences were identified between the ones with and without prebiotic addition. Accordingly, the value of the inhibition zone for those without prebiotic addition (13.18±7.403) was significantly lower than that of cutoff values of 20 with a significant difference of -6.820 (t= -6.514, df: 49, P=0.000). Moreover, the inhibition effect of prebiotics (inulin and starch) against S. typhi at 37 °C for 24 h in part dish glucose as control, only the mean of inulin was found to be significantly lower than that of the cutoff value of 18 with the mean difference of -3.900 (t=-4.115, df: 49, P=0.000). Other prebiotics of glucose and starch in 24 h showed negative inhibition. Probiotics are live microorganisms that have beneficial host effects by enhancing microbial balance in the intestine, whereas prebiotics are indigestible food components having beneficial effects by enhancing the activity and growth of one or more colonic bacteria. Lactobacillus filtrates had considerable effects against the test S. typhi isolates.
کلیدواژه‌ها
Antibacterial activity؛ Prebiotic؛ Probiotic؛ S. typhi
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مراجع
  1. Kant R, Singh V, Nath G, Awasthi SK, Agarwal A. Design, synthesis and biological evaluation of ciprofloxacin tethered bis-1, 2, 3-triazole conjugates as potent antibacterial agents. Eur J Med Chem. 2016;124:218-28.
  2. Sen A, Bandopadhyay S, Misri J, Chauhan H, Kumar PA, Vaid R, et al. Antimicrobial resistance in humans and livestock population in India. Indian J Anim Sci. 2022;92(6):665-81.
  3. Erum S, Fasih F, Fatima A. Frequency of salmonella typhi among bacteremic isolates and their susceptibility pattern against azithromycin. Rawal Med J. 2019;44(1):4-.
  4. Shrestha KL, Pant ND, Bhandari R, Khatri S, Shrestha B, Lekhak B. Re-emergence of the susceptibility of the Salmonella spp. isolated from blood samples to conventional first line antibiotics. Antimicrob Resist Infect Control. 2016;5(1):1-5.
  5. Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, Salminen SJ, et al. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol. 2017;14(8):491-502.
  6. Zaidi A, Mirrett S, McDonald JC, Rubin EE, McDonald LC, Weinstein MP, et al. Controlled comparison of bioMerieux VITAL and BACTEC NR-660 systems for detection of bacteremia and fungemia in pediatric patients. J Clin Microbiol. 1997;35(8):2007-12.
  7. Brooks GF, Carroll KC, Butel J, Morse S, Mietzner T, Jawetz M. Adelberg’s medical microbiology. Sultan Qaboos Univ Med J. 2007;7:273.
  8. Nicula N-O, Lungulescu E-M, Ieropoulos IA, Rimbu GA, Csutak O. Nutrients Removal from Aquaculture Wastewater by Biofilter/Antibiotic-Resistant Bacteria Systems. Water. 2022;14(4):607.
  9. Ling TK, Tam P, Liu Z, Cheng AF. Evaluation of VITEK 2 rapid identification and susceptibility testing system against gram-negative clinical isolates. J. Clin. Microbiol. 2001;39(8):2964-6.
  10. Moin S, Zeeshan M, Laiq S, Raheem A, Zafar A. Use of pefloxacin as a surrogate marker to detect ciprofloxacin susceptibility in Salmonella enterica serotypes Typhi and Paratyphi A. JPMA J Pak Med Assoc. 2020;70(1):96.
  11. Ołdak A, Zielińska D, Rzepkowska A, Kołożyn-Krajewska D. Comparison of antibacterial activity of Lactobacillus plantarum strains isolated from two different kinds of regional cheeses from Poland: Oscypek and Korycinski cheese. BioMed Res Int. 2017;2017.
  12. Moore JC, DeVries JW, Lipp M, Griffiths JC, Abernethy DR. Total protein methods and their potential utility to reduce the risk of food protein adulteration. Comprehensive Rev Food Sci Food Safety. 2010;9(4):330-57.
  13. Zhou H, Yang L, Gui P, Grice CR, Song Z, Wang H, et al. Ga-doped ZnO nanorod scaffold for high-performance, hole-transport-layer-free, self-powered CH3NH3PbI3 perovskite photodetectors. Sol Energy Mater Sols. 2019;193:246-52.
  14. Ragavendran P, Sophia D, Arul Raj C, Gopalakrishnan V. Functional group analysis of various extracts of Aerva lanata (L.,) by FTIR spectrum. Pharmacologyonline. 2011;1:358-64.
  15. Śliżewska K, Chlebicz-Wójcik A. The in vitro analysis of prebiotics to be used as a component of a synbiotic preparation. Nutrients. 2020;12(5):1272.
  16. Le Bastard Q, Chapelet G, Javaudin F, Lepelletier D, Batard E, Montassier E. The effects of inulin on gut microbial composition: a systematic review of evidence from human studies. Eur J Clin Microbiol Infect Dis. 2020;39(3):403-13.
  17. Jao L, McDougall D. The collaborative teacher inquiry project: A purposeful professional development initiative. Can J Educ. 2015;38(1):1-22.
  18. Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc. 2008;3(2):163-75.
  19. Ramadan A. Epidemiological and genetic study of typhoidal fever cause. Iraq: University of Mustansiriya; 2006.
  20. Bhatta D, Bangtrakulnonth A, Tishyadhigama P, Saroj S, Bandekar J, Hendriksen RS, et al. Serotyping, PCR, phage‐typing and antibiotic sensitivity testing of Salmonella serovars isolated from urban drinking water supply systems of Nepal. Lett Appl Microbiol. 2007;44(6):588-94.
  21. Chabuck ZAG, Hadi BH, Hindi NKK. Evaluation of Antimicrobial Activity of Different Aquatic Extracts Against Bacterial Isolates from UTI in Babylon Province, Iraq. J Pure Appl Microbiol. 2018;12(2):693-700.
  22. Dróżdż M, Małaszczuk M, Paluch E, Pawlak A. Zoonotic potential and prevalence of Salmonella serovars isolated from pets. Infect Ecol Epidemiology. 2021;11(1):1975530.
  23. Le Doare K, Bielicki J, Heath PT, Sharland M. Systematic review of antibiotic resistance rates among gram-negative bacteria in children with sepsis in resource-limited countries. J Pediatric Infect Dis Soc. 2015;4(1):11-20.
  24. Klemm EJ, Shakoor S, Page AJ, Qamar FN, Judge K, Saeed DK, et al. Emergence of an extensively drug-resistant Salmonella enterica serovar Typhi clone harboring a promiscuous plasmid encoding resistance to fluoroquinolones and third-generation cephalosporins. MBio. 2018;9(1):e00105-18.
  25. Nagpal R, Mainali R, Ahmadi S, Wang S, Singh R, Kavanagh K, et al. Gut microbiome and aging: Physiological and mechanistic insights. Nutr Healthy Aging. 2018;4(4):267-85.
  26. Zacharof M, Lovitt R. Bacteriocins produced by lactic acid bacteria a review article. Apcbee Procedia. 2012;2:50-6.
  27. Abdel-Daim A, Hassouna N, Hafez M, Ashor MSA, Aboulwafa MM. Antagonistic activity of Lactobacillus isolates against Salmonella typhi in vitro. BioMed Res Int. 2013;2013.
  28. Shoaib M, Shehzad A, Omar M, Rakha A, Raza H, Sharif HR, et al. Inulin: Properties, health benefits and food applications. Carbohydr Polym. 2016;147:444-54.
  1. Karimi R, Azizi MH, Ghasemlou M, Vaziri M. Application of inulin in cheese as prebiotic, fat replacer and texturizer: A review. Carbohydr Polym. 2015;119:85-100.
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