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Aziz Anah, S, Aziz Anah, S. (1402). New Recording of Toxoplasma gondii in Wild Tortoise Testudo graeca Using Nested PCR Method. سامانه مدیریت نشریات علمی, 78(3), 1029-1034. doi: 10.22092/ari.2022.360398.2579
S Aziz Anah; S Aziz Anah. "New Recording of Toxoplasma gondii in Wild Tortoise Testudo graeca Using Nested PCR Method". سامانه مدیریت نشریات علمی, 78, 3, 1402, 1029-1034. doi: 10.22092/ari.2022.360398.2579
Aziz Anah, S, Aziz Anah, S. (1402). 'New Recording of Toxoplasma gondii in Wild Tortoise Testudo graeca Using Nested PCR Method', سامانه مدیریت نشریات علمی, 78(3), pp. 1029-1034. doi: 10.22092/ari.2022.360398.2579
Aziz Anah, S, Aziz Anah, S. New Recording of Toxoplasma gondii in Wild Tortoise Testudo graeca Using Nested PCR Method. سامانه مدیریت نشریات علمی, 1402; 78(3): 1029-1034. doi: 10.22092/ari.2022.360398.2579

New Recording of Toxoplasma gondii in Wild Tortoise Testudo graeca Using Nested PCR Method

Archives of Razi Institute
مقاله 31، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 1029-1034    اصل مقاله (390.64 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.360398.2579
نویسندگان
S Aziz Anah1؛ S Aziz Anah* 2
1General Directorate of Education at Al-Qadisiyah, AL-Diwaniyah, Iraq
2Department of Biology, Faculty of Education,University of AL-Qadisiyah, AL-Diwaniyah, Iraq
چکیده
Toxoplasmosis is one of the most widespread zoonotic diseases, especially in warm and humid areas, and affects all mammals, including humans and many herbivores and carnivores. The present study investigated the Toxoplasma gondii (T. gondii) parasite in tortoises for the first time in Iraq using PCR technology. A total of 28 tortoises/Testudo graeca (T. graeca) were collected between October 2018 and March 2019 from the study stations and then sent to the Animal House, which belongs to the Department of Biology, Faculty of Education, University of AL-Qadisiyah, Iraq, to perform the dissection. The body cavity was opened, and all organs were removed. The tortoises’ liver, heart, and brain were removed and kept at -20ºC until use. Afterward, the samples were subjected to DNA extraction. The Nested-PCR technique was implemented using two pairs of primers, and then the PCR products were analyzed using 1.5% agarose gel electrophoresis. The amplification of the gene during the first cycle indicated that 10 samples gave positive results with a total percentage of (11.9%), including five liver samples, three heart samples, and two brain samples (17.85%, 10.71%, and 7.14%, respectively). On the other hand, during the second cycle of the reaction, the amplification of the gene was obtained in seven samples (8.33%). The highest percentage of the presence of the gene was recorded in the tortoises’ liver (14.28%) and the lowest in their brain (3.57%). This study is among the first to investigate the molecular detection of T. gondii in wild tortoises (T. graeca) in Iraq. The findings imply that tortoises have a role in transmitting T. gondii and are believed to acquire infection by feeding on small invertebrate animals or plants contaminated with the oocysts of the parasite.
کلیدواژه‌ها
Iraq؛ Nested-PCR؛ Testudo graeca؛ Tortoise
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مراجع
  1. Anah SA, Al-Mayali HMH. Molecular detection of Toxoplasma gondii and Cryptosporidium spp. of some snakes in AL-Diwaniyah province/Iraq. EurAsian J Biosci. 2018;12(1):19-26.
  2. Gazzonis AL, Veronesi F, Di Cerbo AR, Zanzani SA, Molineri G, Moretta I, et al. Toxoplasma gondii in small ruminants in Northern Italy - prevalence and risk factors. Ann Agric Environ Med. 2015;22(1):62-8.
  3. Nasiri V, Teymurzadeh S, Karimi G, Nasiri M. Molecular detection of Toxoplasma gondii in snakes. Exp Parasitol. 2016;169:102-6.
  4. Boyer KM, Holfels E, Roizen N, Swisher C, Mack D, Remington J, et al. Risk factors for Toxoplasma gondii infection in mothers of infants with congenital toxoplasmosis: Implications for prenatal management and screening. Am J Obstet Gynecol. 2005;192(2):564-71.
  5. Karatepe B, Babür C, Karatepe M, Kiliç S, Dündar B. Prevalence of Toxoplasma gondii antibodies and intestinal parasites in stray cats from Nigde, Turkey. Ital J Anim Sci. 2008;7(1):113-8.
  6. El Mouden E, Slimani T, Kaddour KB, Lagarde F, Ouhammou A, Bonnet X. Testudo graeca graeca feeding ecology in an arid and overgrazed zone in Morocco. J Arid Environ. 2006;64(3):422-35.
  7. Lagarde F, Bonnet X, Corbin J, Henen B, Nagy K, Mardonov B, et al. Foraging behaviour and diet of an ectothermic herbivore: Testudo horsfieldi. Ecography. 2003;26(2):236-42.
  8. Liesegang A, Hatt JM, Wanner M. Influence of different dietary calcium levels on the digestibility of Ca, Mg and P in Hermann's tortoises (Testudo hermanni). J Anim Physiol Anim Nutr (Berl). 2007;91(11-12):459-64.
  9. Pellitteri-Rosa D, Sacchi R, Galeotti P, Marchesi M, Fasola M. Do Hermann's tortoises (Testudo hermanni) discriminate colours? An experiment with natural and artificial stimuli. Ital J Zool. 2010;77(4):481-91.
  10. Al-Khalidi KAH. Detection of Toxoplasmagondii in Domestic Avian in Middle Euphrates Region and cats in AL-Diwaniya province by Serological and Molecular Techniques. Iraq: University of AL-Qadisiyah. College of Education. Department of Biology; 2013.
  11. Khan A, Su C, German M, Storch GA, Clifford DB, Sibley LD. Genotyping of Toxoplasma gondii strains from immunocompromised patients reveals high prevalence of type I strains. J Clin Microbiol. 2005;43(12):5881-7.
  12. Albert J, Fenyo EM. Simple, sensitive, and specific detection of human immunodeficiency virus type 1 in clinical specimens by polymerase chain reaction with nested primers. J Clin Microbiol. 1990;28(7):1560-4.
  13. Kompalic-Cristo A, Frotta C, Suarez-Mutis M, Fernandes O, Britto C. Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood. Parasitol Res. 2007;101(3):619-25.
  14. Mullis KB. The unusual origin of the polymerase chain reaction. Sci Am. 1990;262(4):56-65.
  15. Findal G, Barlinn R, Sandven I, Stray-Pedersen B, Nordbo SA, Samdal HH, et al. Toxoplasma prevalence among pregnant women in Norway: a cross-sectional study. APMIS. 2015;123(4):321-5.
  16. Montoya JG. Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J Infect Dis. 2002;185 Suppl 1:S73-82.
  17. Stone WB, Manwell RD. Toxoplasmosis in cold-blooded hosts. J Protozool. 1969;16(1):99-102.
  18. Ansari MJ, Jasim SA, Taban TZ, Bokov DO, Shalaby MN, Al-Gazally ME, et al. Anticancer Drug-Loading Capacity of Green Synthesized Porous Magnetic Iron Nanocarrier and Cytotoxic Effects Against Human Cancer Cell Line. J Clust Sci. 2023;34(1):467-77.
  19. Feitosa TF, Brasil AWdL, Parentoni RN, Vilela VLR, Nety TFL, Pena HFdJ. Anti-Toxoplasma gondii antibodies in mammals, birds and reptiles at the zoological-botanical park in João Pessoa, Paraíba, Brazil. Arq Inst Biol. 2018;84.
  20. Olegovich Bokov D, Jalil AT, Alsultany FH, Mahmoud MZ, Suksatan W, Chupradit S, et al. Ir-decorated gallium nitride nanotubes as a chemical sensor for recognition of mesalamine drug: a DFT study. Mol Simul. 2022;48(5):438-47.
  21. Sachse K, Frey J. Methods in molecular biology, PCR detection of microbial pathogens, Humanpress Inc. Totowa. 2008;216:746-55.
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