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New recording of Toxoplasma gondii in Wild Tortoise Testudo graeca using Nested PCR method | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 03 آذر 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.360398.2579 | ||
| نویسندگان | ||
Saad Aziz Anah  1؛ Sadiya Aziz Anah2
| ||
| 1General Directorate of Education at Al-Qadisiyah, AL-Diwaniyah, Iraq. | ||
| 2Department of biology. Faculty of Education ,University of AL-Qadisiyah, AL-Diwaniyah, Iraq | ||
| چکیده | ||
| Toxoplasmosis is one of the most widespread Zoonotic diseases, especially in warm and humid areas, and affects all Mammalia, including humans and many herbivores and carnivores. The present study investigated the Toxoplasma gondii parasite in Iraqi tortoises for the first time in Iraq using PCR technology. A total of 28 (T. graeca) were collected between October 2018 and March 2019 from Study stations and then sent to Animal House, which belongs to the Department of Biology, to perform the dissection. The body cavity was opened, and all organs were removed. Tortoises' liver, heart and brain are removed and kept at -20 ºC until used. Then the samples were subjected to DNA extraction. The Nested PCR technique was then implemented using two pairs of primers, and then PCR products were analyzed using %1.5 agarose gel electrophoresis. The amplification of the gene during the first cycle indicated that 10 samples gave positive results with a total percentage of( %11.9). It includes five liver samples, three heart samples, and two brain samples (%17.85%10.71, and %7.14, respectively). While the amplification of the gene was obtained in seven samples( %8.33) during the second cycle of the reaction, The highest percentage of the presence of the gene had been recorded in the liver of the tortoises (%14.28 ) and the lowest in the brain( %3.57). The study is the first data on the molecular detection of T.gondii in wild tortoise Testudo graeca in Iraq; these results show that tortoises have a role in the transmission of T.gondii and are believed to acquire infection by feeding them on small invertebrate animals or plants contaminated with the oocysts of the parasite. | ||
| کلیدواژه‌ها | ||
| Tortoise؛ Testudo graeca؛ N-PCR؛ Iraq | ||
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آمار تعداد مشاهده مقاله: 19 |
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