اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,043
تعداد مقالات34,036
تعداد مشاهده مقاله46,399,192
تعداد دریافت فایل اصل مقاله77,471,057
Taher, H. J, Kamel, F. H. (1401). Prevalence and Phylogenetic Analysis of Fusobacterium nucleatum and Virulent Factor FadA among Ulcerative Colitis Precancerous and Colorectal Carcinoma Patients in the Iraqi Kurdish Population. سامانه مدیریت نشریات علمی, 78(1), 445-452. doi: 10.22092/ari.2022.360307.2570
H. J Taher; F. H Kamel. "Prevalence and Phylogenetic Analysis of Fusobacterium nucleatum and Virulent Factor FadA among Ulcerative Colitis Precancerous and Colorectal Carcinoma Patients in the Iraqi Kurdish Population". سامانه مدیریت نشریات علمی, 78, 1, 1401, 445-452. doi: 10.22092/ari.2022.360307.2570
Taher, H. J, Kamel, F. H. (1401). 'Prevalence and Phylogenetic Analysis of Fusobacterium nucleatum and Virulent Factor FadA among Ulcerative Colitis Precancerous and Colorectal Carcinoma Patients in the Iraqi Kurdish Population', سامانه مدیریت نشریات علمی, 78(1), pp. 445-452. doi: 10.22092/ari.2022.360307.2570
Taher, H. J, Kamel, F. H. Prevalence and Phylogenetic Analysis of Fusobacterium nucleatum and Virulent Factor FadA among Ulcerative Colitis Precancerous and Colorectal Carcinoma Patients in the Iraqi Kurdish Population. سامانه مدیریت نشریات علمی, 1401; 78(1): 445-452. doi: 10.22092/ari.2022.360307.2570

Prevalence and Phylogenetic Analysis of Fusobacterium nucleatum and Virulent Factor FadA among Ulcerative Colitis Precancerous and Colorectal Carcinoma Patients in the Iraqi Kurdish Population

Archives of Razi Institute
مقاله 55، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 445-452    اصل مقاله (650.22 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.360307.2570
نویسندگان
H. J Taher* 1؛ F. H Kamel2
1Department of Medical Laboratory Technology, Erbil Health and Medical Technical College, Erbil Polytechnic University, Erbil, Iraq
2Department of Medical Laboratory Technology, Medical Technical Institute, Erbil Polytechnic University, Erbil, Iraq
چکیده
Fusobacterium nucleatum is considered one of the main risk factors that play a key role in the promotion and progression of colorectal carcinoma. The main goal of this study is to find out the association between the prevalence of various subtypes of Fusobacterium nucleatum with inflammation and colorectal cancer progression, in addition to screening the positive ratio of the possession of the FadA gene. One hundred tissue samples were collected from healthy individuals and patients from colonoscopy and surgical operation biopsies. The patients were categorized into (Ulcerative colitis, precancerous colitis and colorectal carcinoma) according to their colonoscopy and histopathology examination reports. Molecular detection of Fusobacteriumnucleatum and FadA gene was performed via PCR and gel electrophoresis, and then phylogenetic analysis for Fusobacterium nucleatum was done using 16S rRNA partial sequencing based on specific primers. The results showed significant differences among the four groups regarding the prevalence of Fusobacterium nucleatum. The most prevalent subtype was Fusobacterium nucleatum subtype animalis, which constitutes 7 out of 17 samples. The ratio of the FadA-positive gene was 20% among the Fusobacterium nucleatum-positive cases. This finding suggested a strong correlation between Fusobacterium nucleatum and colon inflammation and cancer progression steps, and Fusobacterium nucleatum subtype animalis was the most prevalent subtype.
کلیدواژه‌ها
Fusobacterium nucleatum؛ FadA؛ Colorectal Carcinoma
سایر فایل های مرتبط با مقاله
مراجع
  1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: Cancer J Clin. 2018;68(6):394-424.
  2. Degett TH, Dalton SO, Christensen J, Søgaard J, Iversen LH, Gögenur I. Mortality after emergency treatment of colorectal cancer and associated risk factors—a nationwide cohort study. Int J Colorectal Dis. 2019;34(1):85-95.
  3. Schreuders EH, Wieten E, Kuipers EJ, Spaander MC. Optimizing fecal immunochemical testing for colorectal cancer screening. Clin Gastroenterol Hepatol. 2017;15(10):1498-9.
  4. Al-Saigh TH, Al-Bayati SA, Abdulmawjood SA, Ahmed FA. Descriptive Study of Colorectal Cancer in Iraq, 1999-2016. ACMM. 2019;41(1):81-5.
  5. Lynch SV, Pedersen O. The human intestinal microbiome in health and disease. N Engl J Med. 2016;375(24):2369-79.
  6. Yu LC-H, Wang J-T, Wei S-C, Ni Y-H. Host-microbial interactions and regulation of intestinal epithelial barrier function: From physiology to pathology. World J Gastrointest Pathophysiol. 2012;3(1):27.
  7. Sender R, Fuchs S, Milo R. Revised estimates for the number of human and bacteria cells in the body. PLoS biology. 2016 Aug 19;14(8):e1002533.
  8. Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature. 2010;464(7285):59-65.
  9. Sommer F, Anderson JM, Bharti R, Raes J, Rosenstiel P. The resilience of the intestinal microbiota influences health and disease. Nat Rev Microbiol. 2017;15(10):630-8.
  10. Nagao-Kitamoto H, Kitamoto S, Kuffa P, Kamada N. Pathogenic role of the gut microbiota in gastrointestinal diseases. Intest Res. 2016;14(2):127.
  11. Schwabe RF, Jobin C. The microbiome and cancer. Nature Reviews Cancer. 2013;13(11):800-12.
  12. Abreu MT, Peek Jr RM. Gastrointestinal malignancy and the microbiome. Gastroenterology. 2014;146(6):1534-46. e3.
  13. Rich JN. Cancer stem cells in radiation resistance. Cancer Res. 2007;67(19):8980-4.
  14. Moore W. Moore LV-1994-. The bacteria of periodontal diseases. Periodontol 2000. 2000;5:66-77.
  15. Fragkioudakis I, Riggio MP, Apatzidou DA. Understanding the microbial components of periodontal diseases and periodontal treatment-induced microbiological shifts. J Med Microbiol. 2021;70(1):001247.
  16. Han YW, Ikegami A, Rajanna C, Kawsar HI, Zhou Y, Li M, et al. Identification and characterization of a novel adhesin unique to oral fusobacteria. J Bacteriol. 2005;187(15):5330-40.
  17. Datorre JG, de Carvalho AC, Guimaraes DP, Reis RM. The Role of Fusobacterium nucleatum in Colorectal Carcinogenesis. Pathobiology. 2021;88(2):127-40.
  18. Payne MS, Bayatibojakhi S. Exploring preterm birth as a polymicrobial disease: an overview of the uterine microbiome. Front Immunol. 2014;5:595.
  19. Payne MS, Newnham JP, Doherty DA, Furfaro LL, Pendal NL, Loh DE, et al. A specific bacterial DNA signature in the vagina of Australian women in midpregnancy predicts high risk of spontaneous preterm birth (the Predict1000 study). Am J Obstet Gynecol. 2021;224(2):206.
  20. Ribot S, Gal K, Goldblat MV, Eslami HH. The role of anaerobic bacteria in the pathogenesis of urinary tract infections. J Urol. 1981;126(6):852-3.
  21. Xu R, Deebel N, Casals R, Dutta R, Mirzazadeh M. A New Gold Rush: A Review of Current and Developing Diagnostic Tools for Urinary Tract Infections. Diagnostics. 2021;11(3):479.
  22. Signat B, Roques C, Poulet P, Duffaut D. Role of Fusobacterium nucleatum in periodontal health and disease. Curr Issues Mol Biol. 2011;13(2):25-36.
  23. Lamont RJ, Koo H, Hajishengallis G. The oral microbiota: dynamic communities and host interactions. Nat Rev Microbiol. 2018;16(12):745-59.
  24. Zhang Y, Wang X, Li H, Ni C, Du Z, Yan F. Human oral microbiota and its modulation for oral health. Biomed Pharmacother. 2018;99:883-93.
  25. Nair V. Oral bacteria and colorectal pathology. Ann Colorectal Res. 2021;9(2):47-50.
  26. Maitre T, Ok V, Calin R, Lassel L, Canestri A, Denis M, et al. Pyogenic lung abscess in an infectious disease unit: a 20-year retrospective study. Ther Adv Respir Dis. 2021;15:17534666211003012.
  27. Mitsuhashi K, Nosho K, Sukawa Y, Matsunaga Y, Ito M, Kurihara H, et al. Association of Fusobacterium species in pancreatic cancer tissues with molecular features and prognosis. Oncotarget. 2015;6(9):7209.
  28. Yoshida A, Ikegami A. Genetic Transformation of Fusobacterium nucleatum. Periodontal Pathogens: Springer; 2021. p. 43-50.
  29. Fan X, Jin Y, Chen G, Ma X, Zhang L. Gut microbiota dysbiosis drives the development of colorectal cancer. Digestion. 2021;102(4):508-15.
  30. Casasanta MA, Yoo CC, Udayasuryan B, Sanders BE, Umaña A, Zhang Y, et al. Fusobacterium nucleatum host-cell binding and invasion induces IL-8 and CXCL1 secretion that drives colorectal cancer cell migration. Sci Signal. 2020;13(641).
  31. Hashemi Goradel N, Heidarzadeh S, Jahangiri S, Farhood B, Mortezaee K, Khanlarkhani N, et al. Fusobacterium nucleatum and colorectal cancer: A mechanistic overview. J Cell Physiol. 2019;234(3):2337-44.
  32. Kong C, Yan X, Zhu Y, Zhu H, Luo Y, Liu P, et al. Fusobacterium Nucleatum Promotes the Development of Colorectal Cancer by Activating a Cytochrome P450/Epoxyoctadecenoic Acid Axis via TLR4/Keap1/NRF2 Signaling. Cancer Res. 2021;81(17):4485-98.
  33. Xu C, Fan L, Lin Y, Shen W, Qi Y, Zhang Y, et al. Fusobacterium nucleatum promotes colorectal cancer metastasis through miR-1322/CCL20 axis and M2 polarization. Gut microbes. 2021;13(1):1980347.
  1. Nosho K, Sukawa Y, Adachi Y, Ito M, Mitsuhashi K, Kurihara H, et al. Association of Fusobacterium nucleatum with immunity and molecular alterations in colorectal cancer. World J Gastroenterol. 2016;22(2):557-66.
  2. Borozan I, Zaidi SH, Harrison TA, Phipps AI, Zheng J, Lee S, et al. Molecular and Pathology Features of Colorectal Tumors and Patient Outcomes Are Associated with Fusobacterium nucleatum and Its Subspecies animalis. Cancer epidemiology, biomarkers & prevention: a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2022;31(1):210-20.
  3. Lamprinaki D, Garcia-Vello P, Marchetti R, Hellmich C, McCord KA, Bowles KM, et al. Siglec-7 Mediates Immunomodulation by Colorectal Cancer-Associated Fusobacterium nucleatum ssp. animalis. Front Immunol. 2021;12:744184.
  4. Ye X, Wang R, Bhattacharya R, Boulbes DR, Fan F, Xia L, et al. Fusobacterium Nucleatum Subspecies Animalis Influences Proinflammatory Cytokine Expression and Monocyte Activation in Human Colorectal Tumors. Cancer Prev Res. 2017;10(7):398-409.
  5. Kashani N, Bezmin Abadi AT, Rahimi F, Forootan M. FadA-positive Fusobacterium nucleatum is prevalent in biopsy specimens of Iranian patients with colorectal cancer. New Microbes New Infect. 2020;34:100651.
  6. Rubinstein MR, Wang X, Liu W, Hao Y, Cai G, Han YW. Fusobacterium nucleatum promotes colorectal carcinogenesis by modulating E-cadherin/β-catenin signaling via its FadA adhesin. Cell Host Microbe. 2013;14(2):195-206.
 

آمار
تعداد مشاهده مقاله: 2,177
تعداد دریافت فایل اصل مقاله: 6,048


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب