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ارزیابی تنوع ژنتیکی نمونههای Beta vulgaris subsp. maritima با استفاده از نشانگرهای مولکولی هسته و سیتوپلاسم | ||
| چغندرقند | ||
| مقاله 2، دوره 37، شماره 2، مهر 1400، صفحه 139-151 اصل مقاله (1.18 M) | ||
| نوع مقاله: کامل علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/jsb.2022.357147.1295 | ||
| نویسندگان | ||
| عاطفه نصیری1؛ اصغر میرزائی اصل* 2؛ محسن آقایی زاده3؛ علی دلجو2 | ||
| 1دانش آموخته گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه بوعلی سینا. همدان، ایران. | ||
| 2دانشیار گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه بوعلی سینا. همدان، ایران. | ||
| 3استادیار مؤسسه تحقیقات اصلاح و تهیه بذر چغندرقند، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران. | ||
| چکیده | ||
| چغندر ماریتیما (Beta vulgaris ssp. maritima) نقش مهمی در برنامههای بهنژادی چغندرقند دارد و تحقیق در مورد تنوع ژنتیکی آن اهمیت زیادی دارد. تعداد 13 نمونه چغندر ماریتیما شامل 10 نمونه ایرانی و سه نمونه اروپایی در این تحقیق ارزیابی شدند. دو نوع نشانگر مولکولی ISSR و ماهوارک (minisatellite) برای بررسی ژنوم هسته و میتوکندری مورد استفاده قرار گرفت. تعداد 176 مکان با 13 آغازگر شناسایی شد که 147 مورد (84/27 درصد) چندشکلی نشان دادند. نتایج مشخص نمود که در بین نمونهها ارزش محتوای اطلاعات چند شکلی (PIC) به میزان 32درصد است. آغازگرهای (AC)8YT و (GA) 8YC بیشترین میزان ارزش PIC (38%) را داشتند. آغازگر (CT)8RG با بیشترین شاخص نشانگر (MI) میتواند برای تجزیههای بیشتر مورد استفاده قرار گیرد. بر اساس نتایج تجزیههای UPGMA و ANOVA همه نمونهها به دو گروه اصلی تقسیم شدند. تجزیه به مؤلفههای اصلی (PCoA) با دندروگرام تنوع ژنتیکی نشان داد که نمونههای ایرانی و اروپایی از یکدیگر متفاوت هستند. برای مطالعه تنوع سیتوپلاسمی، از چهار نشانگر ماهوارک استفاده شد. نمونهها به چهار گروه میتوکندریایی بر اساس تنوع آغازگرها تقسیم شدند. با توجه به نتایج توالی‌یابی برخی نمونههای ایرانی هتروپلاسمی بودند. | ||
| کلیدواژه‌ها | ||
| تنوع ژنتیکی؛ چغندر ماریتیما؛ ماهوارک؛ نشانگر ISSR؛ هتروپلاسمی | ||
| عنوان مقاله [English] | ||
| Evaluation the genetic diversity of Beta vulgaris subsp. maritima samples using nuclear and cytoplasmic molecular markers | ||
| نویسندگان [English] | ||
| A. Nasiri1؛ A. Mirzaie-asl2؛ M. Aghaeizadeh3؛ A. Deljou2 | ||
| 1Educated of Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran | ||
| 2Associate Professor of Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University of Hamedan, Iran. | ||
| 3Assistant Professor of Sugar Beet Seed Institute(SBSI), Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran. | ||
| چکیده [English] | ||
| Beta vulgaris ssp. Maritima plays a significant role in the breeding programs of sugar beet and evaluation of its genetic diversity is of importance. In this study, thirteen samples of Maritima beet including 10 Iranian and three European accessions were evaluated. Two types of molecular markers including ISSR (inter-simple sequence repeat) and minisatellite were used to characterize the nuclear and mitochondrial genomes. A total of 176 loci were identified based on 13 ISSR primers, of which 147 (84.27%) loci showed polymorphisms. Results showed that among the samples, the value of Polymorphism Information Content (PIC) was 32%. Primers 8YT(AC) and 8YC (GA) had the highest PIC value of 0.38. Primer 8RG(CT) with the highest marker index (MI) could be used for a more detailed analysis. Based on the results of UPGMA and ANOVA analysis, all samples were clustered into two main groups. Principle component analysis (PCoA) with dendrogram of genetic diversity showed that Iranian and European samples are different from each other. In order to study the cytoplasm diversity, four minisatellite markers were used, and the samples were divided into four mitochondrial groups based on the variety of primers. According to the sequencing results, some Iranian samples were heteroplasmic. | ||
| کلیدواژه‌ها [English] | ||
| Beta vulgaris ssp. maritima, Genetic diversity, Heteroplasmy, ISSR marker, Minisatellite | ||
| مراجع | ||
|
Alem D, Narancio R, Dellavalle PD, Rebuffo M, Zarza R, Dalla Rizza M. Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. Ciencia Investigacion Agraria., 2011; 38: 453-461. Arjmand MN, Farsinejad K, Alimoradi I, Aghaeizadeh M, Katal B. Pre-breeding for major disease resistances in sugar beet in Iran. International beta genetic resources workshop and world beta network conference; Izmir, Turkey; 1996. Arumuganathan K, Earle ED, Nuclear DNA content of some important plant species, Plant Molecular Biology. 1991; 1: 208-218. Barzen E, Mechelke W, Ritter E, Schulte-Kappert E, Salamini F. An extended map of the sugar beet genome containing RFLP and RAPD loci, Theoretical and Applied Genetics. 1995; 90: 189-193. Bhagyawant S, Srivastava N. Genetic fingerprinting of chickpea (Cicer arietinum L.) germplasm using ISSR markers and their relationships, African Journal of Biotechnology. 2008; 7: 4428–4431. Biancardi E, Campbell LG, Skaracis GN, De Biaggi M. Genetics and breeding of sugar beet, Science Publishers Inc, (Enfield NH) 2005; pp. 392. Cai D, Kleine M, Kifle S, Harloff HJ, Sandal NN. Positional cloning of a gene for nematode resistance in sugar beet, Science. 1997; 275: 832-834. Daniell H, Chase C. Molecular Biology and Biotechnology of Plant Organelles: Chloroplasts and Mitochondria, Springer, 2004; pp. 659. Desplanque B, Viard F, Bernard J, Forcioli D, Saumitou- Laprade P, Cuguen J, Dijk HV. The linkage disequilibrium between chloroplast DNA and mitochondrial DNA haplotypes in Beta vulgaris ssp. maritima (L.): the usefulness of both genomes for population genetic studies, Molecular Ecology. 2000; 9: 141-154. Dos Santos L, de Oliveira E, dos Santos Silva A, de Carvalho F, Costa J, Gomes Pádua J. ISSR markers as a tool for the assessment of genetic diversity in Passiflora, Biochemistry Genetic. 2011; 49: 540-554. Dumolin- Lapegue S, Pemonge MH, Petit RJ, Association between chloroplast and mitochondrial lineages in oaks, Molecular Biology and Evolution. 1998; 15: 1321-1331. Fenart S, Arnaud JF, De Cauwer I, Cuguen J. Nuclear and cytoplasmic genetic diversity in weed beet and sugar beet accessions compared to wild relatives: new insights into the genetic relationships within the Beta vulgaris complex species, Theoretical and Applied Genetics. 2008; 116: 1063-1077. Fenart S, Touzet P, Arnaud JF, Cuguen J. Emergence of gynodioecy in wild beet (Beta vulgaris ssp. maritima L.): a genealogical approach using chloroplastic nucleotide sequences, Proceedings: Biological Sciences. 2006; 273: 1391-1398. Ghislain M, Zhang D, Fajardo D, Huamán Z, Hijmans R. Marker-assisted sampling of the cultivated Andean potato Solanum phureja collection using RAPD markers, Genetic Resources and Crop Evolution. 1999; 46: 547-555. Grimmer MK, Trybush S, Hanley S, Francis, SA, Karp A, Asher MJC. An anchored linkage map for sugar beet based on AFLP, SNP and RAPD markers and QTL mapping of a new source of resistance to Beet necrotic yellow vein virus, Theoretical and Applied Genetics. 2007; 114: 1151-1160. Hashizume T, Shimamoto I, Hirai M, Construction of a linkage map and QTL analysis of horticultural traits for watermelon Citrullus lanatus (THUNB.) MATSUM & NAKAI] using RAPD, RFLP and ISSR markers, Theoretical and Applied Genetics. 2003; 106: 779-785. He F, Kang D, Ren Y, Qu L, Zhen Y, Gu H. Genetic diversity of the natural populations of Arabidopsis thaliana in China. Heredity. 2007; 99: 423-431. Jaccard P. Nouvelles researches sur la distribution florale, Bulletin de la Societe Vaudoise des Sciences Naturelles, 1908; 44: 223-270. Joshi-Saha A, Gopalakrishna T. Inheritance and tagging of gene regulating flowering time in the green manure crop Sesbania rostrata (Bremek. & Obrem.), Molecular Breeding. 2007; 20: 389-399. Kato K, Akashi Y, Tanaka K, Wako T, Masuda M. Genetic characterization of east and south Aslan melon by the analysis of molecular polymorphisms and morphological characters. Acta II, International Symposium In Melons; 2003. Kozyrenko MM, Gontcharova SB, Gontcharov AA. Analysis of the genetic structure of Rhodiola rosea (Crassulaceae) using inter-simple sequence repeat (ISSR) polymorphisms. Flora. 2011; 206: 691-696. Kumar LS. DNA markers in plant improvement: an overview. Biotechnology Advances, 1999; 17: 143-182. Li J, Schulz B, Stich B. Population structure and genetic diversity in elite sugar beet germplasm investigated with SSR markers. Euphytica. 2010; 175: 35-42. Li JM, Jin ZX. Genetic structure of endangered Emmenopterys henryi Oliv. based on ISSR polymorphism and implications for its conservation. Genetica. 2008; 133: 227-234. MaoQian W, ZeDong WU, HuaZhong W. Genetic diversity of major sugar beet varieties from three regions of china with SRAP markers. Acta Agronomica Sinica. 2011; 37: 811-819. Martins- Lopes P, Lima-Brito J, Gomes S, Meirinhos J, Santos L et al. RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification. Genetic Resources and Crop Evolution. 2007; 54: 117-128. McCauley DE. The use of chloroplast DNA polymorphism in studies of gene flow in plants. Trends in Ecology & Evolution. 1995; 10: 198-202. Meyer W, Michell TG, Freedman EZ, Vilgalys R. Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans. Journal of Clinical Biology and Biochemistry. 1993; 31: 2274-2280. Nishizawa S, Kubo T, Mikami T. Variable number of tandem repeat loci in the mitochondrial genomes of beets. Current Genetics. 2000; 37: 34-38. Peakall R, Smouse PE. GenAlEx 6.5: genetic analysis in Excel, Population genetic software for teaching and research-an update. Bioinformatics. 2012; 28: 2537-2539. Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding. 1996; 2: 225-238. Sankar AA, Moore GA. Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map. Theoretical and Applied Genetics. 2001; 102: 206-214. Sehgal D, Rajpal V, Raina S, Sasanuma T, Sasakuma T. Assaying polymorphism at DNA level for genetic diversity diagnostics of the safflower (Carthamus tinctorius L.) world germplasm resources. Genetica. 2009; 135: 457-470. Semagn K, Bjornstad A, Stedje B, Bekele E. Comparison of multivariate methods for the analysis of genetic resources and adaptation in Phytolacca dodecandra using RAPD. Theoretical and Applied Genetics. 2000; 101: 1145-1154. Sharma SK, Koneox MR, Ellis THN. AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis. Theoretical and Applied Genetics. 1999; 93: 751-758. Sivaprakash K, Prashanth S, Mohanty B, Parida A. Genetic diversity of black gram (Vigna mungo) landraces as evaluated by amplified fragment length polymorphism markers. Curr Sci. 2004; 86: 1411-1416. Smulders MJ, Esselink GD, Everaert I, De Riek J, Vosman B. Characterisation of sugar beet (Beta vulgaris L. ssp. vulgaris) varieties using microsatellite markers. BMC Genetics. 2010; 11: 41. Sneath PH, Sokal RR. Numerical taxonomy: the principles and practice of numerical classification, (San Francisco, USA) Freeman Publisher Inc. 1973; pp. 573. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Reseache. 1994; 22: 4673-4680. Venkateswarlu M, Urs SR, Nath BS, Shashidhar H, Maheswaran M, Veeraiah TM, Sabitha MG. A first genetic linkage map of mulberry (Morus spp.) using RAPD, ISSR, and SSR markers and pseudotestcross mapping strategy. Tree Genet & Genomes. 2006; 3: 15-24. Verma S, Rana T. Genetic diversity within and among the wild populations of Murraya koenigii (L.) Spreng., as revealed by ISSR analysis. Biochemical Systematics and Ecology. 2011; 39: 139-144. Vijayan K, Srivatsava P, Nair C, Awasthi A, Tikader A, Sreenivasa B, Urs SR. Molecular characterization and identification of markers associated with yield traits in mulberry using ISSR markers. Plant breeding. 2006; 125: 298-301. Wu K, Jones R, Dannaeberger L, Scolnik PK. Detection of microsatellite polymorphism without cloning. Nucleic Acid Research. 1994; 22: 3257-3258. | ||
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