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تغییرات القایی سطح پلوئیدی بر تولید لیگنان‌ها، فلاونوئیدها و فنولیک اسیدها در کشت کالوس گیاه دارویی کتان سفید (Linum album Boiss.) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| دوره 30، شماره 1 - شماره پیاپی 59، شهریور 1401، صفحه 97-107 اصل مقاله (871.37 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2022.358704.1420 | ||
| نویسندگان | ||
| سیده زینب رضوی کشمیری1؛ ندا جوادیان2؛ محسن شریفی* 3؛ قاسم کریم زاده4 | ||
| 1دانش‌آموخته کارشناسی ارشد، گروه زیست‌شناسی گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران | ||
| 2دانش‌آموخته دکتری، گروه ژنتیک و بهنژادی گیاهی، پردیس کشاورزی، دانشگاه تربیت مدرس، تهران | ||
| 3استاد، گروه زیست‌شناسی گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران | ||
| 4استاد، گروه ژنتیک و بهنژادی گیاهی، پردیس کشاورزی، دانشگاه تربیت مدرس، تهران | ||
| چکیده | ||
| اتوپلی پلوئیدی القائی به عنوان روشی مؤثر در افزایش تولید برخی متابولیتهای ثانوی در گیاهان دارویی در نظر گرفته میشود. کتان سفید یک گیاه دارویی ارزشمند و سرشار از برخی ترکیبات لیگنان با خاصیت ضدویروسی و ضدسرطانی مانند پودوفیلوتوکسین است. در این تحقیق، اثر القاء اتوتتراپلوئیدی بر تولید برخی لیگنان ها، فنولیک اسیدها و فلاونوئیدهای مشتق شده از مسیر فنیل پروپانوئید، در کشت کالوس کتان سفید بررسی شد. کالوس‌های تتراپلوئید از برگ کتان سفید اتوتتراپلوئید القایی به‌دست آمد و پایداری پلوئیدی آن بعد از چندین بار زیرکشت به وسیله فلوسایتومتری تأیید شد. دو برابر شدن کروموزومی یا تتراپلوئیدی باعث افزایش معنی دار تولید لیگنان (پینورسینول)، فولیک اسیدها (کافئیک اسید، کوماریک اسید و فرولیک اسید) و فلاونوئیدها (میریستین، نارینجنین و کاتچین) اندازه گیری شده در کشت کالوس شد، در حالی که روی تولید سایر لیگنان ها (لاریسیرسینول و پودوفیلوتوکسین) اثر قابل توجهی نداشت. این نتایج نشان داد که دو برابر شدن سطح پلوئیدی میتواند به عنوان یک روش مؤثر برای افزایش تولید برخی از متابولیت های ثانوی در کالوس کتان سفید استفاده شود. | ||
| کلیدواژه‌ها | ||
| پودوفیلوتوکسین؛ ترکیبات فنولی؛ سطح پلوئیدی؛ فلوسایتومتری؛ کتان سفید | ||
| عنوان مقاله [English] | ||
| Ploidy level-induced changes in production of lignans, flavonoids, and phenolic acids in Linum album callus culture | ||
| نویسندگان [English] | ||
| Z.A. Razavi Keshmiri1؛ N. Javadian2؛ M. Sharifi3؛ G. Karimzadeh4 | ||
| 1M. Sc. graduate, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 2Ph.D. graduate, Dept. Plant Genetics and Breeding, College of Agriculture, Tarbiat Modares University, Tehran, I.R.Iran | ||
| 3Prof., Dept. Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, I.R. Iran | ||
| 4Prof., Department of Plant Genetics and Breeding, College of Agriculture, Tarbiat Modares University, Tehran, I.R. Iran | ||
| چکیده [English] | ||
| Induced autopolyploidy is noticed as an effective method for increasing the production of some secondary metabolites in medicinal plans. Linum album is a valuable medicinal plant which is rich in some lignan compounds with antiviral and anticancer properties such as, podophyllotoxin. In this study, the effect of autotetraploid induction on the production of some lignans, phenolic acids, and flavonoids derived from the phenylpropanoid pathway was investigated through L. album callus culture. Tetraploid calli were obtained from the leaves of the induced autotetraploid L. album, and its ploidy stability was confirmed using flow cytometry after several subcultures. Chromosome doubling or tetraploidy caused a significant increase in the production of lignan (pinoresinol), folic acids (caffeic acid, coumaric acid, and ferulic acid) and flavonoids (myricetin, naringenin, and catechin) measured in the callus culture, while it had no significant effect on the production of other lignans (larisiresinol and podophyllotoxin). It was concluded that autotetraploid induction could be an effective method to increasing the production of some secondary metabolites in L. album callus. | ||
| کلیدواژه‌ها [English] | ||
| Flow cytometry, Linum album, Phenolic compound, Ploidy level | ||
| مراجع | ||
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