Study of local black Iraqi goats genotypes for the Cytb gene

M. Owaid, Jaafar; Y Yousief, Muntaha; J. Abdulrda, Ali; Y. Ayied, Asaad

doi:10.22092/ari.2022.359888.2499

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	Study of local black Iraqi goats genotypes for the Cytb gene
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 26 مهر 1401
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359888.2499
نویسندگان
Jaafar M. Owaid ؛ Muntaha Y Yousief؛ Ali J. Abdulrda؛ Asaad Y. Ayied
Animal Production Department, College of Agriculture, University of Basrah
چکیده
Goats are the earliest domesticated ruminants. The local goat, Capra hircus, is considered one of the most important animals globally to provide good livestock production under harsh environmental conditions. The study aimed to detect the genetic structures of the local Iraqi goats bred in the central and southern regions of the country and to investigate the possibility of benefiting from genetic structures to construct improvement programs to increase the productivity of these animals. Blood samples were obtained from 15 domestic black goats. A total of 10 ml of each animal were placed in plastic containers of 10 ml. The DNA was extracted and sent to the laboratories of Juan Ju University in the People's Republic of China to analyze the sequences of the nitrogenous bases of the Cytb gene. The results showed the presence of a genetic morphology for a segment of 670 base pairs for all studied samples, and 15 sequences of this strain were recorded in the gene bank under the following accession numbers (LC496367.1:1- (LC496353.1:1). The sequences of nitrogenous bases for this piece of the gene and registered in the gene bank of some international goat breeds were used to compare them with the sequences of black Iraqi goats to analyze the phylogenetic tree, calculating genetic distance, studying haplotypes and calculating neutrality. The results showed the presence of one mutation in the studied segment of the Cytb gene, which has a size of 670 bp. The mutation in base 46 of the studied gene, which is a mutation that converts from the purine group to the pyrimidine group, i.e., the shift from the nitrogen leaders A<C, and in all the studied samples. Which led to the transformation of the amino acid Asparagine into Histidine, where 233 amino acids were obtained, dominated by the amino acid Isoleucine and Leucine over the other amino acids, at a rate of (14.34%, 11.21%), respectively. The phylogenetic tree showed the existence of two main branches, one of which included the local black Iraqi goat breed, and the other included all the international breeds under comparison.
کلیدواژه‌ها
local black؛ Iraqi goats genotypes؛ Cytb gene

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Study of local black Iraqi goats genotypes for the Cytb gene