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Nadeem, Z, Iqbal, J, Kausar, S, Gasmi Benahmed, A, Noor, S, Khan, F., Saleem, I, Munir, N, Riaz, M, Akram, M, Oladoye, P., Salim, S., Abed, A., Elbossaty, W., Gasmi, A. (1401). Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis. سامانه مدیریت نشریات علمی, 77(6), 2053-2060. doi: 10.22092/ari.2022.359856.2486
Z Nadeem; J Iqbal; S Kausar; A Gasmi Benahmed; S Noor; F. S Khan; I Saleem; N Munir; M Riaz; M Akram; P. O Oladoye; S. A Salim; A. A Abed; W. F Elbossaty; A Gasmi. "Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2053-2060. doi: 10.22092/ari.2022.359856.2486
Nadeem, Z, Iqbal, J, Kausar, S, Gasmi Benahmed, A, Noor, S, Khan, F., Saleem, I, Munir, N, Riaz, M, Akram, M, Oladoye, P., Salim, S., Abed, A., Elbossaty, W., Gasmi, A. (1401). 'Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis', سامانه مدیریت نشریات علمی, 77(6), pp. 2053-2060. doi: 10.22092/ari.2022.359856.2486
Nadeem, Z, Iqbal, J, Kausar, S, Gasmi Benahmed, A, Noor, S, Khan, F., Saleem, I, Munir, N, Riaz, M, Akram, M, Oladoye, P., Salim, S., Abed, A., Elbossaty, W., Gasmi, A. Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis. سامانه مدیریت نشریات علمی, 1401; 77(6): 2053-2060. doi: 10.22092/ari.2022.359856.2486

Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis

Archives of Razi Institute
مقاله 5، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2053-2060  XML اصل مقاله (446.03 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359856.2486
نویسندگان
Z Nadeem1؛ J Iqbal1؛ S Kausar1؛ A Gasmi Benahmedorcid 2؛ S Noororcid 3؛ F. S Khan4؛ I Saleem5؛ N Munir6؛ M Riaz7؛ M Akram4؛ P. O Oladoye8؛ S. A Salim9؛ A. A Abed9؛ W. F Elbossaty10؛ A Gasmi email orcid 11
1Department of Microbiology, University of Central Punjab Lahore, Pakistan
2Académie Internationale de Médecine Dentaire Intégrative, Paris, France
3Institute of Molecular Biology and Biotechnology, BZ University, Multan, Pakistan
4Department of Eastern Medicine, Government College University Faisalabad, Pakistan
5Department of Pharmacy, University of Poonch Rawalakot, Pakistan
6Department of Biochemistry, Government College University Faisalabad, Pakistan
7Department of Allied Health Sciences, University of Sargodha, Pakistan
8Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St, Miami, FL 33199
9Al-Mussiab Technical College, Al-Furat Al-Awsat Technical University, Iraq
10Biochemistry Department, Damietta University, Egypt
11Société Francophone de Nutrithérapie et de Nutrigénétique Appliquée, Villeurbanne, France
چکیده
Tuberculosis is one of the predominant infectious diseases causing significant deaths worldwide. Detection of Mycobacterium tuberculosis bacilli (MTB) using culture media was officially recognized by World Health Organization. However, there is a significant limitation in the authenticity of evaluation for its effectiveness on clinically important attributes. GeneXpert detects the presence of Mycobacterium tuberculosis (M. tuberculosis) based on the detection of nucleic acid and is able to identify the resistance of both isoniazid (INH) and Rifampicin (RIF) drugs. In this technique, DNA amplification is done using the GeneXpert instrument in the suspected sample with a specific reagent cartridge. Although GeneXpert is a rapid technique compared to other diagnostic tools for MTB identification due to false-negative results, the culture media technique is still considered the gold standard in detecting M. tuberculosis. The current study was designed to evaluate the comparative efficacies of GeneXpert and the solid culture media technique in identifying MTB. Sputum samples of 250 (n=250) suspected tuberculosis (TB) patients were investigated using both diagnostic techniques. The results revealed that out of the 250 suspected patients, 30 (12%) samples were positive with the culture media technique, while only 17 (6.8%) samples showed positive results with GeneXpert. Culture tests and GeneXpert are not equally efficient in detecting M. tuberculosis. The current study's findings showed that the culture-based detection method for M. tuberculosis is more efficient and reliable than GeneXpert.
کلیدواژه‌ها
M. tuberculosis؛ GeneXpert؛ Culture media؛ Acid-fast Bacilli
مراجع
  1. WHO. Global tuberculosis report 2013: World Health Organization; 2013 [Available from: https://apps.who.int/iris/handle/10665/91355.
  2. Leung NHL. Transmissibility and transmission of respiratory viruses. Nat Rev Microbiol. 2021;19(8):528-45.
  3. Mollel E, Lekule I, Lynen L, Decroo T. Effect of reliance on Xpert MTB/RIF on time to treatment and multidrug-resistant tuberculosis treatment outcomes in Tanzania: a retrospective cohort study. Int Health. 2019;11(6):520-7.
  4. Garg SK, Tiwari RP, Tiwari D, Singh R, Malhotra D, Ramnani VK, et al. Diagnosis of tuberculosis: available technologies, limitations, and possibilities. J Clin Lab Anal. 2003;17(5):155-63.
  5. WHO. WHO meeting report of a technical expert consultation: non-inferiority analysis of Xpert MTB/RIF Ultra compared to Xpert MTB/RIF: World Health Organization; 2017 [Available from: https://www.who.int/publications-detail-redirect/WHO-HTM-TB-2017.04.
  6. Shah D, Gupta Y. Role of Molecular Tests for Diagnosis of Tuberculosis in Children. Pediatric Oncall Journal. 2015;12(1):1-3.
  7. Kapalamula TF, Thapa J, Akapelwa ML, Hayashida K, Gordon SV, Hang' Ombe BM, et al. Development of a loop-mediated isothermal amplification (LAMP) method for specific detection of Mycobacterium bovis. PLoS Negl Trop Dis. 2021;15(1):e0008996.
  8. Singh A, Prasad R, Balasubramanian V, Gupta N. Drug-Resistant Tuberculosis and HIV Infection: Current Perspectives. HIV AIDS (Auckl). 2020;12:9-31.
  9. Chen J, Wang S, Zhang Y, Su X, Wu J, Shao L, et al. Rv1985c, a promising novel antigen for diagnosis of tuberculosis infection from BCG-vaccinated controls. BMC Infect Dis. 2010;10:273.
  10. Comas I, Coscolla M, Luo T, Borrell S, Holt KE, Kato-Maeda M, et al. Out-of-Africa migration and Neolithic coexpansion of Mycobacterium tuberculosis with modern humans. Nat Genet. 2013;45(10):1176-82.
  11. Negi SS, Khan SFB, Gupta S, Pasha ST, Khare S, Lal S. Comparison of the conventional diagnostic modalities, bactec culture and polymerase chain reaction test for diagnosis of tuberculosis. Indian J Med Microbiol. 2005;23(1):29-33.
  12. Held M, Laubscher M, Zar HJ, Dunn RN. GeneXpert polymerase chain reaction for spinal tuberculosis: an accurate and rapid diagnostic test. Bone Joint J. 2014;96-B(10):1366-9.
  13. Tripathi K, Tripathi PC, Nema S, Shrivastava AK, Dwiwedi K, Dhanvijay AK. Modified Petroff's Method: an excellent simplified decontamination technique in comparison with Petroff's Method. Int J Recent Trends Sci Technol. 2014;10:461-4.
  14. ECDC. Handbook on tuberculosis laboratory diagnostic methods in the European Union: European Centre for Disease Prevention and Control; 2018. Available from: https://www.ecdc.europa.eu/en/publications-data/handbook-tuberculosis-laboratory-diagnostic-methods-european-union-updated-2018.
  15. Takarada Y, Kodera T, Kobayashi K, Nakajima C, Kawase M, Suzuki Y. Rapid detection of rifampicin-resistant Mycobacterium tuberculosis, based on isothermal DNA amplification and DNA chromatography. J Microbiol Methods. 2020;177:106062.
  1. Aricha SA, Kingwara L, Mwirigi NW, Chaba L, Kiptai T, Wahogo J, et al. Comparison of GeneXpert and line probe assay for detection of Mycobacterium tuberculosis and rifampicin-mono resistance at the National Tuberculosis Reference Laboratory, Kenya. BMC Infect Dis. 2019;19(1):852.
  2. Mechal Y, Benaissa E, El Mrimar N, Benlahlou Y, Bssaibis F, Zegmout A, et al. Evaluation of GeneXpert MTB/RIF system performances in the diagnosis of extrapulmonary tuberculosis. BMC Infect Dis. 2019;19(1):1069.
  3. Li S, Liu B, Peng M, Chen M, Yin W, Tang H, et al. Diagnostic accuracy of Xpert MTB/RIF for tuberculosis detection in different regions with different endemic burden: A systematic review and meta-analysis. PLoS One. 2017;12(7):e0180725.
  4. Sah SK, Bhattarai PR, Shrestha A, Dhami D, Guruwacharya D, Shrestha R. Rifampicin-resistant Mycobacterium tuberculosis by GeneXpert MTB/RIF and Associated Factors Among Presumptive Pulmonary Tuberculosis Patients in Nepal. Infect Drug Resist. 2020;13:2911-9.
  5. El-Helbawy R, AMA T. eneXpert Mycobacterial tuberculosis/rifampicin: predictors of successful performance. Egypt J Chest Dis Tuberc. 2020;69(1):33.
  6. Zetola NM, Shin SS, Tumedi KA, Moeti K, Ncube R, Nicol M, et al. Mixed Mycobacterium tuberculosis complex infections and false-negative results for rifampin resistance by GeneXpert MTB/RIF are associated with poor clinical outcomes. J Clin Microbiol. 2014;52(7):2422-9.
  7. Rufai SB, Kumar P, Singh A, Prajapati S, Balooni V, Singh S. Comparison of Xpert MTB/RIF with line probe assay for detection of rifampin-monoresistant Mycobacterium tuberculosis. J Clin Microbiol. 2014;52(6):1846-52.
  8. WHO. Automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF assay for the diagnosis of pulmonary and extra-pulmonary TB in adults and children: policy update: World Health Organization; 2022 [Available from: https://apps.who.int/iris/handle/10665/112472.
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