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Nadeem, Z, Iqbal, J, Kausar, S, Gasmi Benahmed, A, Noor, S, Khan, F., Saleem, I, Munir, N, Riaz, M, Akram, M, Oladoye, P., Salim, S., Abed, A., Elbossaty, W., Gasmi, A. (1401). Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis. سامانه مدیریت نشریات علمی, 77(6), 2065-2072. doi: 10.22092/ari.2022.359856.2486
Z Nadeem; J Iqbal; S Kausar; A Gasmi Benahmed; S Noor; F. S Khan; I Saleem; N Munir; M Riaz; M Akram; P. O Oladoye; S. A Salim; A. A Abed; W. F Elbossaty; A Gasmi. "Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2065-2072. doi: 10.22092/ari.2022.359856.2486
Nadeem, Z, Iqbal, J, Kausar, S, Gasmi Benahmed, A, Noor, S, Khan, F., Saleem, I, Munir, N, Riaz, M, Akram, M, Oladoye, P., Salim, S., Abed, A., Elbossaty, W., Gasmi, A. (1401). 'Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis', سامانه مدیریت نشریات علمی, 77(6), pp. 2065-2072. doi: 10.22092/ari.2022.359856.2486
Nadeem, Z, Iqbal, J, Kausar, S, Gasmi Benahmed, A, Noor, S, Khan, F., Saleem, I, Munir, N, Riaz, M, Akram, M, Oladoye, P., Salim, S., Abed, A., Elbossaty, W., Gasmi, A. Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis. سامانه مدیریت نشریات علمی, 1401; 77(6): 2065-2072. doi: 10.22092/ari.2022.359856.2486

Comparative Analysis of the Efficacies of the GeneXpert and Solid Culture Media Techniques in the Diagnosis of Mycobacterium Tuberculosis

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مقاله 7، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2065-2072    اصل مقاله (444.62 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359856.2486
نویسندگان
Z Nadeem1؛ J Iqbal1؛ S Kausar1؛ A Gasmi Benahmed2؛ S Noor3؛ F. S Khan4؛ I Saleem5؛ N Munir6؛ M Riaz7؛ M Akram4؛ P. O Oladoye8؛ S. A Salim9؛ A. A Abed9؛ W. F Elbossaty10؛ A Gasmi* 11
1Department of Microbiology, University of Central Punjab Lahore, Pakistan
2Académie Internationale de Médecine Dentaire Intégrative, Paris, France
3Institute of Molecular Biology and Biotechnology, BZ University, Multan, Pakistan
4Department of Eastern Medicine, Government College University Faisalabad, Pakistan
5Department of Pharmacy, University of Poonch Rawalakot, Pakistan
6Department of Biochemistry, Government College University Faisalabad, Pakistan
7Department of Allied Health Sciences, University of Sargodha, Pakistan
8Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St, Miami, FL 33199
9Al-Mussiab Technical College, Al-Furat Al-Awsat Technical University, Iraq
10Biochemistry Department, Damietta University, Egypt
11Société Francophone de Nutrithérapie et de Nutrigénétique Appliquée, Villeurbanne, France
چکیده
Tuberculosis is one of the predominant infectious diseases causing significant deaths worldwide. Detection of Mycobacterium tuberculosis bacilli (MTB) using culture media was officially recognized by World Health Organization. However, there is a significant limitation in the authenticity of evaluation for its effectiveness on clinically important attributes. GeneXpert detects the presence of Mycobacterium tuberculosis (M. tuberculosis) based on the detection of nucleic acid and is able to identify the resistance of both isoniazid (INH) and Rifampicin (RIF) drugs. In this technique, DNA amplification is done using the GeneXpert instrument in the suspected sample with a specific reagent cartridge. Although GeneXpert is a rapid technique compared to other diagnostic tools for MTB identification due to false-negative results, the culture media technique is still considered the gold standard in detecting M. tuberculosis. The current study was designed to evaluate the comparative efficacies of GeneXpert and the solid culture media technique in identifying MTB. Sputum samples of 250 (n=250) suspected tuberculosis (TB) patients were investigated using both diagnostic techniques. The results revealed that out of the 250 suspected patients, 30 (12%) samples were positive with the culture media technique, while only 17 (6.8%) samples showed positive results with GeneXpert. Culture tests and GeneXpert are not equally efficient in detecting M. tuberculosis. The current study's findings showed that the culture-based detection method for M. tuberculosis is more efficient and reliable than GeneXpert.
کلیدواژه‌ها
M. tuberculosis؛ GeneXpert؛ Culture media؛ Acid-fast Bacilli
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مراجع
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