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The GGC medium reduces the DNA fragmentation of human spermatozoa via in vitro activation | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 16 شهریور 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.359720.2461 | ||
| نویسندگان | ||
Noor Kareem Kadhim  1؛ Ahmed Hussein Zwamel2
| ||
| 1Department of medical laboratory technologies, Al-Farabi University College, Baghdad, Iraq | ||
| 2Radiology Techniques Department, College of Medical Technology, The Islamic University, Najaf, Iraq. | ||
| چکیده | ||
| In vitro sperm stimulation by adding certain chemicals has been discovered to become one of the most significant techniques for dealing with sperm DNA fragmentation, which is one of the leading causes of male infertility. GGC medium is an invented triple antioxidants-containing medium (10 mM/ml green tea extract, 10 mM/ml Glutathione, 60 mM/ml Vitamin C, sodium pyruvate 0.01g/L, and 10% human serum albumin applied to 1L Ringer solution) for in vitro human sperm activation. This study aimed to evaluate the quality of the human sperm DNA after in vitro activation using a GGC medium. Two hundred semen samples were enrolled in this study. The samples were split into three groups before activation by swim-up technique: control group (G1) with no activation medium, G2 and G3 activated with Ferticult flushing medium, and GGC medium, accordingly. Then, the sperm DNA fragmentation index (DFI) was assessed pre- and post- swim-up activation. When comparing pre-activation to post-activation, the findings of DNA fragmentation revealed a significant increase in pre-activation. Furthermore, relative to the other treatment groups, there was a strong significant (P<0.05) reduction of DFI in samples activated with GGC medium. When comparing DFI between pre-activation and post-activation, G2 and G3 showed a substantial reduction (P<0.05). According to the findings of this study, both mediums may reduce DNA fragmentation; however, the GGC medium shows the most significant outcomes when contrasted to the Ferticult medium given to activate spermatozoa in vitro. | ||
| کلیدواژه‌ها | ||
| GGC medium؛ ferticult medium؛ DNA fragmentation. spermatozoa | ||
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آمار تعداد مشاهده مقاله: 16 |
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