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Kareem Kadhim, N, Hussein Zwamel, A. (1402). The GGC Medium Reduces the DNA Fragmentation of Human Spermatozoa via in vitro Activation. سامانه مدیریت نشریات علمی, 78(2), 709-714. doi: 10.22092/ari.2022.359720.2461
N Kareem Kadhim; A Hussein Zwamel. "The GGC Medium Reduces the DNA Fragmentation of Human Spermatozoa via in vitro Activation". سامانه مدیریت نشریات علمی, 78, 2, 1402, 709-714. doi: 10.22092/ari.2022.359720.2461
Kareem Kadhim, N, Hussein Zwamel, A. (1402). 'The GGC Medium Reduces the DNA Fragmentation of Human Spermatozoa via in vitro Activation', سامانه مدیریت نشریات علمی, 78(2), pp. 709-714. doi: 10.22092/ari.2022.359720.2461
Kareem Kadhim, N, Hussein Zwamel, A. The GGC Medium Reduces the DNA Fragmentation of Human Spermatozoa via in vitro Activation. سامانه مدیریت نشریات علمی, 1402; 78(2): 709-714. doi: 10.22092/ari.2022.359720.2461

The GGC Medium Reduces the DNA Fragmentation of Human Spermatozoa via in vitro Activation

Archives of Razi Institute
مقاله 26، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 709-714    اصل مقاله (409.37 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359720.2461
نویسندگان
N Kareem Kadhim1؛ A Hussein Zwamel* 2
1Department of Medical Laboratory Technologies, Al-Farabi University College, Baghdad, Iraq
2Radiology Techniques Department, College of Medical Technology, The Islamic University, Najaf, Iraq
چکیده
In vitro sperm stimulation by the addition of certain chemicals has become one of the most significant techniques for dealing with sperm DNA fragmentation, which is one of the leading causes of male infertility. The GGC medium is an invented triple antioxidant-containing medium (10 mM/ml green tea extract, 10 mM/ml glutathione, 60 mM/ml vitamin C, sodium pyruvate 0.01g/L, and 10% human serum albumin applied to 1L Ringer solution) for in vitro human sperm activation. This study aimed to evaluate the quality of the human sperm DNA after in vitro activation using a GGC medium. For this purpose, 200 semen samples were employed in this study. Before activation by swim-up technique, the samples were split into three groups, consisting of a control group (G1) with no activation medium, and G2 and G3 groups activated with Ferticult flushing medium and GGC medium, respectively. Afterward, the sperm DNA fragmentation index (DFI) was assessed pre- and post-swim-up activation. Based on the comparison of pre- and post-activation stages, the findings of DNA fragmentation revealed a significant increase at the pre-activation stage. Furthermore, relative to the other treatment groups, there was a strong significant (P<0.05) reduction of DFI in samples activated with GGC medium. The G2 and G3 showed a substantial reduction of DFI at the post-activation stage, compared to the pre-activation stage (P<0.05). According to the findings, both mediums can reduce DNA fragmentation; however, the GGC medium showed the most significant outcomes, compared to the Ferticult medium used for the in vitro activation of spermatozoa.
کلیدواژه‌ها
DNA fragmentation؛ Ferticult medium؛ GGC medium؛ Spermatozo
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مراجع
  1. Lombardo F, Sansone A, Romanelli F, Paoli D, Gandini L, Lenzi A. The role of antioxidant therapy in the treatment of male infertility: an overview. Asian J Androl. 2011;13(5):690.
  2. Mohan ZH, Al-Hilli NM, Selman MO. Effects of Vaginal Misoprostol after Intrauterine Insemination IUI. IJEIR. 2019;9(2):39-54.
  3. Idowu OO. Green tea extract and reproduction: A review. E3 J Med Res. 2017;6:001-6.
  4. Dadashpour Davachi N, Norouzi E, Didarkhah M, Eslampanah M, Hablolvarid MH. In vitro Production of Grivet Monkey (C hlorocebus aethiops) Embryo. Iran J Vet Med. 2022;16(3):257-64.
  5. Abshenas J, Babaei H, ZAREI MH, Allahbakhshi A, Sharififar F, editors. The effects of green tea (Camellia sinensis) extract on mouse semen quality after scrotal heat. Vet Res Forum. 2011.
  6. Roychoudhury S, Agarwal A, Virk G, Cho C-L. Potential role of green tea catechins in the management of oxidative stress-associated infertility. Reprod Biomed Online. 2017;34(5):487-98.
  7. Kolesnikova L, Kurashova N, Bairova T, Dolgikh M, Ershova O, Dashiev B, et al. Role of glutathione-S-transferase family genes in male infertility. Bull Exp Biol Med. 2017;163(5):643-5.
  8. Buhling K, Schumacher A, Zu Eulenburg C, Laakmann E. Influence of oral vitamin and mineral supplementation on male infertility: a meta-analysis and systematic review. Reprod Biomed Online. 2019;39(2):269-79.
  9. Rahman SU, Huang Y, Zhu L, Feng S, Khan IM, Wu J, et al. Therapeutic role of green tea polyphenols in improving fertility: a review. Nutrients. 2018;10(7):834.
  10. Dutta S, Majzoub A, Agarwal A. Oxidative stress and sperm function: A systematic review on evaluation and management. Arab J Urol. 2019;17(2):87-97.
  11. Mahmoudi R, Azizi A, Abedini S, Jahromi VH, Abidi H, Barmak MJ. Green tea improves rat sperm quality and reduced cadmium chloride damage effect in spermatogenesis cycle. J Med Life Sci. 2018;11(4):371.
  12. Fafula R, Onufrovych O, Iefremova U, Melnyk O, Nakonechnyi I, Vorobets D, et al. Glutathione content in sperm cells of infertile men.  Regul Mech Biosyst. 2017;2(8):157-61.
  13. Chávez JC, Hernández-González EO, Wertheimer E, Visconti PE, Darszon A, Treviño CL. Participation of the Cl−/HCO3− exchangers SLC26A3 and SLC26A6, the Cl− channel CFTR, and the regulatory factor SLC9A3R1 in mouse sperm capacitation. Biol Reprod. 2012;86(1):1-14.
  14. Agarwal A, Majzoub A, Esteves SC, Ko E, Ramasamy R, Zini A. Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios. Transl Androl Urol. 2016;5(6):935.
  15. Sciorio R, Thong J, Pickering S. Comparison of the development of human embryos cultured in either an EmbryoScope or benchtop incubator. J Assist Reprod Gene. 2018;35(3):515-22.
  16. Tejada RI, Mitchell JC, Norman A, Marik JJ, Friedman S. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril. 1984;42(1):87-91.
  17. Custers IM. Intrauterine insemination: Fine-tuning a treatment. 2013.
  18. Zwamel A, Kadhim N, editors. New medium from green tea extract, glutathione and vitamin C (GGC) for activation of human spermatozoa in vitro. Human Reproduction; 2019: Oxford Univ Press Great Clarendon St, Oxford Ox2 6dp, England.
  19. van der Linden M, Buckingham K, Farquhar C, Kremer JA, Metwally M. Luteal phase support for assisted reproduction cycles. Cochrane Database Syst Rev. 2015;(7).
  20. Barbonetti A, Calogero A, Balercia G, Garolla A, Krausz C, La Vignera S, et al. The use of follicle stimulating hormone (FSH) for the treatment of the infertile man: position statement from the Italian Society of Andrology and Sexual Medicine (SIAMS). J Endocrinol Invest. 2018;41(9):1107-22.
  21. Barati E, Nikzad H, Karimian M. Oxidative stress and male infertility: Current knowledge of pathophysiology and role of antioxidant therapy in disease management. Cell Mol Life Sci. 2020;77(1):93-113.
  22. Opuwari CS, Henkel RR. An update on oxidative damage to spermatozoa and oocytes. Biomed Res Int. 2016;2016.
  23. Shirata C, Kaneko J, Inagaki Y, Kokudo T, Sato M, Kiritani S, et al. Near-infrared photothermal/photodynamic therapy with indocyanine green induces apoptosis of hepatocellular carcinoma cells through oxidative stress. Sci Rep. 2017;7(1):1-8.
  24. Samardzija M, Getz I, Lojkic M, Valpotic H, Djuricic D. Optimization of sperm for in vitro production of bovine embryos. SOJ Vet Sci. 2015;1(2):1-7.
  25. Harrison R. A highly efficient method for washing mammalian spermatozoa. Reproduction. 1976;48(2):347-53.
  26. Zhang N, Duncan FE, Que EL, O’Halloran TV, Woodruff TK. The fertilization-induced zinc spark is a novel biomarker of mouse embryo quality and early development. Sci Rep. 2016;6(1):1-9.
  27. Raimondo S, Gentile T, Gentile M, Donnarumma F, Esposito G, Morelli A, et al. Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein. J Hum Reprod Sci. 2020;13(2):117.
  1. Agarwal A, Majzoub A, Baskaran S, Selvam MKP, Cho CL, Henkel R, et al. Sperm DNA fragmentation: a new guideline for clinicians. World J Mens Health. 2020;38(4):412.
  2. Cyrus A, Kabir A, Goodarzi D, Moghimi M. The effect of adjuvant vitamin C after varicocele surgery on sperm quality and quantity in infertile men: a double blind placebo controlled clinical trial. Int Braz J Urol. 2015;41:230-8.
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