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Tahmasebi Enferadi, Sattar, Salimizadeh, Zahra, Mahjoubi, Frouzandeh. (1402). Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line. سامانه مدیریت نشریات علمی, 12(4), 505-511. doi: 10.22092/jmpb.2022.357131.1435
Sattar Tahmasebi Enferadi; Zahra Salimizadeh; Frouzandeh Mahjoubi. "Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line". سامانه مدیریت نشریات علمی, 12, 4, 1402, 505-511. doi: 10.22092/jmpb.2022.357131.1435
Tahmasebi Enferadi, Sattar, Salimizadeh, Zahra, Mahjoubi, Frouzandeh. (1402). 'Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line', سامانه مدیریت نشریات علمی, 12(4), pp. 505-511. doi: 10.22092/jmpb.2022.357131.1435
Tahmasebi Enferadi, Sattar, Salimizadeh, Zahra, Mahjoubi, Frouzandeh. Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line. سامانه مدیریت نشریات علمی, 1402; 12(4): 505-511. doi: 10.22092/jmpb.2022.357131.1435

Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line

Journal of Medicinal plants and By-product
دوره 12، شماره 4، اسفند 2023، صفحه 505-511    اصل مقاله (1.09 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2022.357131.1435
نویسندگان
Sattar Tahmasebi Enferadi* ؛ Zahra Salimizadeh؛ Frouzandeh Mahjoubi
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
چکیده
Harmaline and harmine, are the major ß-carbolines present in the seeds of the Peganum harmala L. These compounds are known as herbal active principals with potential use in pharmaceutical and medicine. To assess the growth inhibitory effect of phyto-alkaloids, harmaline and harmine, on cancer cell lines. The P. harmala L.’s alkaloids were extracted by acidic/basic extraction method and identified by two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High-Performance Liquid Chromatography (HPLC). breast cancer cell lines, MDA_MB_231, were subjected to different concentration (1–100 μg. mL -1) of the P. harmala extract at different time courses (24h, 48h). Methylthiazol Tetrazolium (MTT) test, the half maximal inhibitory concentration (IC50) and the morphological changes through optical microscopy were evaluated cell lines, the P. harmala extract decreased cell viability in longer time exposure in a dose dependent manner. The more concentrated extract led to higher motility of MDA-MB-231 at 24h.It was observed that 30 μg. mL -1 is the minimum lethal dose that kills approximately 50% of cells at 24 hours in MDA-MB-231 cell line (IC50). The morphological observation ensured the apoptosis nature of P. harmala on cells as their membrane kept intact and no membrane permeabilization was observed. The results revealed that the P. harmala extracts decreased significantly growth rate and cell survival of cancer cell lines. higher growth inhibition of MDA-MB-231 cell line by the P. harmala extract was confirmed. 
کلیدواژه‌ها
Cell viability؛ FT-IR؛ HPLC؛ Harmine؛ Harmaline
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