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Tahmasebi Enferadi, Sattar, Salimizadeh, Zahra, Mahjoubi, Frouzandeh. (1402). Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line. سامانه مدیریت نشریات علمی, 12(4), 505-511. doi: 10.22092/jmpb.2022.357131.1435
Sattar Tahmasebi Enferadi; Zahra Salimizadeh; Frouzandeh Mahjoubi. "Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line". سامانه مدیریت نشریات علمی, 12, 4, 1402, 505-511. doi: 10.22092/jmpb.2022.357131.1435
Tahmasebi Enferadi, Sattar, Salimizadeh, Zahra, Mahjoubi, Frouzandeh. (1402). 'Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line', سامانه مدیریت نشریات علمی, 12(4), pp. 505-511. doi: 10.22092/jmpb.2022.357131.1435
Tahmasebi Enferadi, Sattar, Salimizadeh, Zahra, Mahjoubi, Frouzandeh. Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line. سامانه مدیریت نشریات علمی, 1402; 12(4): 505-511. doi: 10.22092/jmpb.2022.357131.1435

Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line

Journal of Medicinal plants and By-Products
مقاله 21، دوره 12، شماره 4، اسفند 2023، صفحه 505-511    اصل مقاله (1.09 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2022.357131.1435
نویسندگان
Sattar Tahmasebi Enferadi* ؛ Zahra Salimizadeh؛ Frouzandeh Mahjoubi
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
چکیده
Harmaline and harmine, are the major ß-carbolines present in the seeds of the Peganum harmala L. These compounds are known as herbal active principals with potential use in pharmaceutical and medicine. To assess the growth inhibitory effect of phyto-alkaloids, harmaline and harmine, on cancer cell lines. The P. harmala L.’s alkaloids were extracted by acidic/basic extraction method and identified by two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High-Performance Liquid Chromatography (HPLC). breast cancer cell lines, MDA_MB_231, were subjected to different concentration (1–100 μg. mL -1) of the P. harmala extract at different time courses (24h, 48h). Methylthiazol Tetrazolium (MTT) test, the half maximal inhibitory concentration (IC50) and the morphological changes through optical microscopy were evaluated cell lines, the P. harmala extract decreased cell viability in longer time exposure in a dose dependent manner. The more concentrated extract led to higher motility of MDA-MB-231 at 24h.It was observed that 30 μg. mL -1 is the minimum lethal dose that kills approximately 50% of cells at 24 hours in MDA-MB-231 cell line (IC50). The morphological observation ensured the apoptosis nature of P. harmala on cells as their membrane kept intact and no membrane permeabilization was observed. The results revealed that the P. harmala extracts decreased significantly growth rate and cell survival of cancer cell lines. higher growth inhibition of MDA-MB-231 cell line by the P. harmala extract was confirmed. 
کلیدواژه‌ها
Cell viability؛ FT-IR؛ HPLC؛ Harmine؛ Harmaline
مراجع
  1. Harvey A.L. Medicines from nature: are natural products still relevant to drug discovery? Trends Pharmacol Sci. 1999; 20:196–8.
  2. Mahmoudian M., Jalilpour H., Salehian P. Toxicity of Peganum harmala: Review and a Case Report. Iran J Pharmaco Therap. 2002;1:1- 4.
  3. Imad A., Sajad F., Haroon K., Philippe J., Michael A., Zhi-Ling Y. Targeting cell cycle by β-carboline alkaloids in vitro: Novel therapeutic prospects for the treatment of cancer,Chemico-Biological Interactions. 2020 ; 330: 109229
  4. Rahimi-Moghaddam P, Ebrahimi SA, Ourmazdi H, Selseleh M, Karjalian M, Haj-Hassani G, et al. In vitro and in vivo activities of Peganum harmala extract against Leishmania major. J Res Med Sci. 2011;16:1032–9.
  5. Azzazy HME-S., Fahmy S.A., Mahdy N.K., Meselhy M.R., Bakowsky U. Chitosan-Coated PLGA Nanoparticles Loaded with Peganum harmala Alkaloids with Promising Antibacterial and Wound Healing Activities. Nanomaterials. 2021; 11(9):2438.
  6. Omar F., Tareq A.M., Alqahtani A.M., Dhama K., Sayeed M.A., Emran T.B., Simal-Gandara J. Plant-Based Indole Alkaloids: A Comprehensive Overview from a Pharmacological Perspective. Molecules. 2021; 26(8):2297.
  7. Herraiz T., González D., Ancín-Azpilicueta C, Arán VJ and Guillén H. β-Carboline alkaloids in Peganum harmala and inhibition of human monoamine oxidase (MAO). Food Chem. Toxicol. 2010;48:839–45.
  8. Jime´nez J., Rivero´n-Negrete L., Abdullaev F., Espinosa-Aguirre J., Rodrıguez-Arnaiz R. Cytotoxicity of the beta-carboline alkaloids harmine and harmaline in human cell assays in vitro. Experimental and toxicologic pathology: Exp Toxicol Pathol. 2008;60::381–389.
  9. Kim H., Sablin S.O., Ramsay R.R. Inhibition of monoamine oxidase A by beta-carboline derivatives. Arch Biochem Biophys. 1997;337:137–42.
  10. Chen Q., Chao R., Chen H., Hou X., Yan H., Zhou S., et al. Antitumor and neurotoxic effects of novel harmine derivatives and structure activity relationship analysis. Int. J. Cancer. 2005;11:675–82.
  11. Siegel R., Naishadham D., Jemal A. Cancer statistics, 2012. CA: CA-Cancer J. Clin. 2012;62:10–29.
  12. Jemal A., Bray F., Center M.M., Ferlay J., Ward E., Forman D. Global cancer statistics. CA-Cancer J. Clin. 2011;61:69–90.
  13. Holliday D., Speirs V. Choosing the right cell line for breast cancer research. Breast Cancer Res. 2011;12;13:215.
  14. Subik K., Lee J-F., Baxter L., Strzepek T., Costello D., Crowley P., et al. The Expression Patterns of ER, PR, HER2, CK5/6, EGFR, Ki-67 and AR by Immunohistochemical Analysis in Breast Cancer Cell Lines. Breast Cancer (Auckl). 2010;4:35–41.
  15. Saleem A., Husheem M., Härkönen P., Pihlaja K. Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit. J Ethnopharmacol. 2002;81:327–36.
  16. Riva L., Coradini D., Di Fronzo G., De Feo V., De Tommasi N., De Simone F., et al. The antiproliferative effects of Uncaria tomentosa extracts and fractions on the growth of breast cancer cell line. Anticancer Res. 2001;21:2457–61.
  17. Hostanska K., Nisslein T., Freudenstein J., Reichling J., Saller R. Cimicifuga racemosa extract inhibits proliferation of estrogen receptor-positive and negative human breast carcinoma cell lines by induction of apoptosis. Breast Cancer Res Treat. 2004;84:151–60.
  18. Shoemaker M, Hamilton B, Dairkee SH, Cohen I and Campbell MJ. In vitro anticancer activity of twelve Chinese medicinal herbs. Phytother Res. 2005;19:649–51.
  19. Cragg G.M., Newman D.J. Plants as a source of anti-cancer agents. J. Ethnopharmacol. 2005;100:72–9.
  20. Newsha Mortazavi, Mahboobeh Heidari, Zohreh Rabiei, sattar tahmasebi, Maryam Monazzah Loading harmine on nanographene changes the inhibitory effects of free harmine against MCF-7 and fibroblast cells. Med Chem Res 2021; 30, 1108–1116.
  21. Funayama Y., Nishio K., Wakabayashi K., Nagao M., Shimoi K., Ohira T., et al. Effects of beta- and gamma-carboline derivatives of DNA topoisomerase activities. Mutat Res. 1996;349:183–91.
  22. Abe A., Yamada H. Harmol induces apoptosis by caspase-8 activation independently of Fas/Fas ligand interaction in human lung carcinoma H596 cells. Anti-cancer Drugs. 2009;20:373–81.
  23. Jo M., Kim TH., Seol D.W., Esplen J.E., Dorko K., Billiar T.R., et al. Apoptosis induced in normal human hepatocytes by tumor necrosis factor-related apoptosis-inducing ligand. Nat Med. 2000;6:564–7.
  24. Gerlier D., Thomasset N. Use of MTT colorimetric assay to measure cell activation. J Immunol Methods. 1986;94:57–63.
  25. Lamchouri F., Zemzami M., Jossang A., Abdellatif A., Israili ZH., Lyoussi B. In vitro cell-toxicity of Peganum harmala alkaloids on cancerous cell-lines. Fitoterapia. 2013;71(1):50–54.
  26. Sobhani A.M., Ebrahimi S.A., Mahmoodiani M. In vitro evaluation of human DNA topo isomerase I inhibition by peganum harmala and its beta carolin alkaloids. J. Pharm. Sci. 2002;5:19-23.
  27. Zhao L., Wink M. The b-carboline alkaloid harmine inhibits telomerase activity of MCF-7 cells by down-regulating hTERT mRNA expression accompanied by an accelerated senescent phenotype. peerj. 2013;174.
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