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Rafiee, Dana, Ebrahimi, Mohammad Ali, Qavami, Nassrin, Zarinpanjeh, Nasim. (1402). The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi. سامانه مدیریت نشریات علمی, 12(4), 397-404. doi: 10.22092/jmpb.2022.358302.1465
Dana Rafiee; Mohammad Ali Ebrahimi; Nassrin Qavami; Nasim Zarinpanjeh. "The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi". سامانه مدیریت نشریات علمی, 12, 4, 1402, 397-404. doi: 10.22092/jmpb.2022.358302.1465
Rafiee, Dana, Ebrahimi, Mohammad Ali, Qavami, Nassrin, Zarinpanjeh, Nasim. (1402). 'The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi', سامانه مدیریت نشریات علمی, 12(4), pp. 397-404. doi: 10.22092/jmpb.2022.358302.1465
Rafiee, Dana, Ebrahimi, Mohammad Ali, Qavami, Nassrin, Zarinpanjeh, Nasim. The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi. سامانه مدیریت نشریات علمی, 1402; 12(4): 397-404. doi: 10.22092/jmpb.2022.358302.1465

The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi

Journal of Medicinal plants and By-Products
مقاله 10، دوره 12، شماره 4، اسفند 2023، صفحه 397-404    اصل مقاله (1.21 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2022.358302.1465
نویسندگان
Dana Rafiee1؛ Mohammad Ali Ebrahimi1؛ Nassrin Qavami2؛ Nasim Zarinpanjeh* 2
1Department of Agricultural Biotechnology, Payam Noor University, Tehran, Iran
2Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
چکیده
Nitraria schoberi L. is a medicinal plant with antioxidant, antifungal, anti-inflammatory and, anti-viral properties. This plant is also tolerant to salinity and drought. The main aim of this study was to perform elicitation in cell suspension culture of N. schoberi to detect the production of total phenolic and flavonoid contents. In order to accomplish this study, hypocotyls and cotyledonary leaves of N. schoberi were cultured in different callus induction treatments. At the next step, the calli from the best callus induction treatment were transferred to cell suspension cultures containing sodium chloride (NaCl) and salicylic acid (SA) alone or in combination. Total phenolic and flavonoid contents in treatments were measured using a spectrophotometer (absorbance at 765 nm and 430 nm, respectively). According to the results, although callus induction was observed in all treatments, the maximum fresh weight (FW) (7.6 g) and dry weight (DW) (3.7 g) were obtained by culturing hypocotyl explants in MS medium supplemented with 2, 4-dichrolophenoxyacetic acid (2, 4-D) at 0.5 mg/L. The highest total phenolic content (62.2 mg/g) and total flavonoid content (48.24 mg/g) were detected in suspension culture containing 100 millimolar (mM) NaCl and 50 micromolar (µM) SA. Elicitation in suspension culture of N. schoberi can be an effective method for increasing the production of secondary metabolites (phenolic and flavonoid compounds).
کلیدواژه‌ها
Callus induction؛ Elicitor؛ Medicinal plants؛ Secondary metabolites؛ Suspension culture
مراجع
 

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