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Taki, E, Jabalameli, F, Mohajeri Tehrani, M. R, Feizabadi, M. M, Beigverdi, R, Emaneini, M. (1401). Microbial Profile and Antibiotic Susceptibility Pattern in Diabetic Patients with Mild, Moderate, and Severe Foot Infections in Tehran. سامانه مدیریت نشریات علمی, 77(5), 1925-1933. doi: 10.22092/ari.2022.359759.2476
E Taki; F Jabalameli; M. R Mohajeri Tehrani; M. M Feizabadi; R Beigverdi; M Emaneini. "Microbial Profile and Antibiotic Susceptibility Pattern in Diabetic Patients with Mild, Moderate, and Severe Foot Infections in Tehran". سامانه مدیریت نشریات علمی, 77, 5, 1401, 1925-1933. doi: 10.22092/ari.2022.359759.2476
Taki, E, Jabalameli, F, Mohajeri Tehrani, M. R, Feizabadi, M. M, Beigverdi, R, Emaneini, M. (1401). 'Microbial Profile and Antibiotic Susceptibility Pattern in Diabetic Patients with Mild, Moderate, and Severe Foot Infections in Tehran', سامانه مدیریت نشریات علمی, 77(5), pp. 1925-1933. doi: 10.22092/ari.2022.359759.2476
Taki, E, Jabalameli, F, Mohajeri Tehrani, M. R, Feizabadi, M. M, Beigverdi, R, Emaneini, M. Microbial Profile and Antibiotic Susceptibility Pattern in Diabetic Patients with Mild, Moderate, and Severe Foot Infections in Tehran. سامانه مدیریت نشریات علمی, 1401; 77(5): 1925-1933. doi: 10.22092/ari.2022.359759.2476

Microbial Profile and Antibiotic Susceptibility Pattern in Diabetic Patients with Mild, Moderate, and Severe Foot Infections in Tehran

Archives of Razi Institute
مقاله 52، دوره 77، شماره 5، آذر و دی 2022، صفحه 1925-1933    اصل مقاله (720.71 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359759.2476
نویسندگان
E Taki1؛ F Jabalameli1؛ M. R Mohajeri Tehrani2؛ M. M Feizabadi1؛ R Beigverdi1، 3؛ M Emaneini* 1، 3
1Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Science Institute, Tehran University of Medical Sciences, Tehran, Iran
3Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran
چکیده
It is estimated that 10-25% of diabetic patients will encounter diabetic foot ulcers (DFU) during their lifetime. This study evaluated the microbiology of DFUs and determined the antibiotic resistance pattern of bacterial isolates based on the severity of wounds and infections in different grades of ulcer. The specimens were collected from115 diabetic foot infections (DFI) deep tissue by needle aspiration and biopsy. The aerobic and anaerobic cultures and antimicrobial susceptibility testing were carried out. The presence of resistance genes including Metallo-beta-lactamases (MBL), extended-spectrum β-lactamase (ESBL), ermA, ermC, and mecA was also determined. A total of 222 microorganisms were isolated. The prevalence of poly-microbial infections was 69.6%. Bacterial isolates comprised 64.2% Gram-positive bacteria (GPB), 33.5% Gram-negative bacteria (GNB), and five isolates of anaerobic bacteria were also detected. The most prevalent GPB and GNB were Staphylococcus spp. (52.2%) and Escherichia coli (33.3%), respectively. The prevalence of poly-microbial infections and GNB was positively associated with increased grades of Wagner and IDSA classifications. Among Staphylococcus aureus isolates, resistance to clindamycin (73.5%), ciprofloxacin (70.6%), and erythromycin (70.6%) were noticeable. GNB was also highly resistant to cephalosporins and ciprofloxacin. ESBL genes were detected in approximately 40% of isolates of Enterobacteriaceae. The prevalence of Erma, ermC, and mecA genes in S. aureus isolates were 8.8%, 32.3%, and 14.7%, respectively. In conclusion, our data suggest that GPBs are the most common isolates from DFIs. Furthermore, with the development of wounds and infection, the prevalence of GNB in DFIs are increased.
کلیدواژه‌ها
Foot infections؛ Antimicrobial Resistance؛ S. aureus؛ ESBL
مراجع
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