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آمار
| تعداد نشریات | 281 |
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| تعداد شماره‌ها | 2,395 |
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Effect of Vitamin D3 on some antioxidant parameters in chilled semen in Awassi Ram | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 22 مرداد 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.359482.2433 | ||
| نویسندگان | ||
Mohammed Hakim Abed Bresm  ؛ Hayder Mohammed Hassan Habeeb
| ||
| Department of Animal Production, College of Agriculture, Al-Qasim Green University, Babylon, Iraq. | ||
| چکیده | ||
| Chilled semen has been used in farm animals to maintain semen viability. However, it might be damaged by reactive oxygen species causing oxidative stress and then reduced semen fertility. The objective of the current study is to evaluate the different concentrations of vitamin D3 as antioxidants in Awassi chilled semen. Two-three ejaculates from three Awassi rams were used in this study. Samples were pooled, diluted with Tris-egg yolk extender (1:10) then aliquots. Three vitamins D3 concentration (T1=0.02, T2=0.004, T3=0.002 gm/ml), as a treatment, and one control without any addition, was added to aliquots. Treatments and control groups were cooled to 5 ºC. Samples were centrifuged at 2000 RPM for 20 minutes at times 0 and 72 following treatment. The seminal plasm was kept in a freezer at 20 ºC until evaluation. Malondialdehyde (MDA), Reactive oxygen species (ROS), Total antioxidants capacity (TAC), Superoxide dismutase (SOD), Catalase (CAT), were used in this study to evaluate the antioxidant activity of vitamin D3. One way analysis of variance repeated measures was used in this study by using the SAS program. The results showed that the TAC, and SOD increased significantly in T1 compared to T0, T1, and T2. In addition, CAT increased significantly in T2 compared to T0, T1, and T3. ROS and MDA was not significantly changed between treatments. Although there were no significant difference in MDA between treatments, but it decreased numerically on T1 compared to other treatments. In conclusion low dose of vitamin D3 has a potential antioxidant capacity which bring a new site to use it to prolong semen storge. | ||
| کلیدواژه‌ها | ||
| chilled semen؛ Awassi ram؛ Vitamin D3؛ antioxidant | ||
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آمار تعداد مشاهده مقاله: 13 |
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