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Ghazy Hawal, R, Talib Bakr, M. (1402). Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields. سامانه مدیریت نشریات علمی, 78(2), 587-592. doi: 10.22092/ari.2022.359468.2424
R Ghazy Hawal; M Talib Bakr. "Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields". سامانه مدیریت نشریات علمی, 78, 2, 1402, 587-592. doi: 10.22092/ari.2022.359468.2424
Ghazy Hawal, R, Talib Bakr, M. (1402). 'Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields', سامانه مدیریت نشریات علمی, 78(2), pp. 587-592. doi: 10.22092/ari.2022.359468.2424
Ghazy Hawal, R, Talib Bakr, M. Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields. سامانه مدیریت نشریات علمی, 1402; 78(2): 587-592. doi: 10.22092/ari.2022.359468.2424

Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields

Archives of Razi Institute
مقاله 11، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 587-592    اصل مقاله (648.72 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359468.2424
نویسندگان
R Ghazy Hawal* ؛ M Talib Bakr
Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
چکیده
Pullorum disease (PD) is one of the most common diseases in the world, with devastating consequences. In the chicken sector, there have been financial losses. It is brought on by Salmonella enteric subspecies serovar Gallinarum biovar pullorum; definitive detection requires culture followed by biochemistry analysis and serotyping. This study aimed to verify the presence of bacteria by culture, biochemical characterization, PCR assay, and sequencing. One hundred samples were collected from 12 broiler chicken flocks of different ages for 8districts of Baghdad province, including cloacal swabs (65), visceral organs (15), and dropping (20). Salmonella colonies were identified by selective culture broth and agar with biochemical description for 75% of the total samples, with a higher incidence in visceral organs than dropping and cloacal swabs. ،The Sequencing and phylogenetic tree analysis of 16S rRNA gene for representative Salmonella isolates. The presence of Salmonella pullorum isolates in global genetic strains; was revealed a matching NCBI isolates similarity of 99.02% with (MF445124.1) and 98% with (MH352164.1), respectively. In the current state of molecular and genetic research, phlyogentic research announced the real presence of Salmonella pullorum in Baghdadprovince's broiler chicken, also showing the phylogentic characteristics and links to some global isolates. The detection of Salmonella pullorum in broiler flocks of the current study extent of health risks to other uninfected birds present in the free range.
کلیدواژه‌ها
Salmonella pullorum؛ Biochemical Tests؛ PCR؛ Phylogenetic Analysis
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