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Alwaily, E. R, Abood, M. S, Al Uobody, R. M. (1401). Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis. سامانه مدیریت نشریات علمی, 78(1), 475-483. doi: 10.22092/ari.2022.359466.2422
E. R Alwaily; M. S Abood; R. M Al Uobody. "Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis". سامانه مدیریت نشریات علمی, 78, 1, 1401, 475-483. doi: 10.22092/ari.2022.359466.2422
Alwaily, E. R, Abood, M. S, Al Uobody, R. M. (1401). 'Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis', سامانه مدیریت نشریات علمی, 78(1), pp. 475-483. doi: 10.22092/ari.2022.359466.2422
Alwaily, E. R, Abood, M. S, Al Uobody, R. M. Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis. سامانه مدیریت نشریات علمی, 1401; 78(1): 475-483. doi: 10.22092/ari.2022.359466.2422

Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis

Archives of Razi Institute
مقاله 59، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 475-483    اصل مقاله (586.49 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359466.2422
نویسندگان
E. R Alwaily* 1؛ M. S Abood2؛ R. M Al Uobody3
1Microbiology Research Group , College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq
2Department of Biology, College of Education for Pure Science, Thi-Qar University, Thi-Qar 64001, Iraq
3College of Pharmacy, Al-Ayen University, Thi-Qar, 64001, Iraq
چکیده
Candida tropical has been found as the most abundant pathogenic yeast species under the group Candida-non-albicans. Despite this, it is taxonomically related to C. albicans and has many of its pathogenic characteristics. Infection with Candida tropicalis is closely associated with many virulence factors encoded by multiple virulence genes. This study aims to diagnose C. tropicalis based on the presence of 18SrRNA and to detect many virulence genes. C. tropicalis isolates were collected from oral candidiasis patients. Children infected with oral thrush ranging in age from infants to 12 years old provided 150 samples. C. albicans (66.68 %), C. tropicalis (13.21 %), C. krusie (9.43 %), C. parapsilosis (7.55 %), and C. glarata were isolated as C. tropicalis types, according to the findings of the present study (2.83%). The presence of the 18SrRNA gene was confirmed in the isolates. All isolates were positive for cph1 and hwp1, while some were positive for sap1 (78.5%) and plb1 genes (71.4%). Using sequences and phylogenetic trees, it was determined that there was negligible genetic variation between local isolates and global strains. These virulence factor genes play a crucial role in developing infections.
کلیدواژه‌ها
Oral candidiasis؛ sap1؛ hwp1؛ cph1؛ plb1
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