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Ali Hameed AL-Dabbagh, A, Ali Ajah, H, Abdul Sattar Salman, J. (1401). Detection of Virulence Factors from Candida spp. Isolated from Oral and Vaginal Candidiasis in Iraqi Patients. سامانه مدیریت نشریات علمی, 78(1), 465-474. doi: 10.22092/ari.2022.359464.2420
A Ali Hameed AL-Dabbagh; H Ali Ajah; J Abdul Sattar Salman. "Detection of Virulence Factors from Candida spp. Isolated from Oral and Vaginal Candidiasis in Iraqi Patients". سامانه مدیریت نشریات علمی, 78, 1, 1401, 465-474. doi: 10.22092/ari.2022.359464.2420
Ali Hameed AL-Dabbagh, A, Ali Ajah, H, Abdul Sattar Salman, J. (1401). 'Detection of Virulence Factors from Candida spp. Isolated from Oral and Vaginal Candidiasis in Iraqi Patients', سامانه مدیریت نشریات علمی, 78(1), pp. 465-474. doi: 10.22092/ari.2022.359464.2420
Ali Hameed AL-Dabbagh, A, Ali Ajah, H, Abdul Sattar Salman, J. Detection of Virulence Factors from Candida spp. Isolated from Oral and Vaginal Candidiasis in Iraqi Patients. سامانه مدیریت نشریات علمی, 1401; 78(1): 465-474. doi: 10.22092/ari.2022.359464.2420

Detection of Virulence Factors from Candida spp. Isolated from Oral and Vaginal Candidiasis in Iraqi Patients

Archives of Razi Institute
مقاله 58، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 465-474    اصل مقاله (479.95 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359464.2420
نویسندگان
A Ali Hameed AL-Dabbagh* ؛ H Ali Ajah؛ J Abdul Sattar Salman
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq
چکیده
Yeast-like fungi (YLF) of the genus Candida are unicellular microorganisms of relatively large size and rounded shape, aerobes, and belong to conditionally pathogenic microorganisms. The genus Candida includes approximately 150 species, which are classified as Deuteromycetes due to the absence of a sexual stage of development. This study aimed to identify virulence factors from Candida spp. isolated from oral and vaginal candidiasis. Fifty-eight oral and vaginal swab specimens were collected from patients, including (28) oral swabs from children and (30) vaginal swabs from different infected women. All isolates were subjected to direct examination, Morphological tests, Germ tube formation, growth at 45ºC, CHROM agar Candida culture, and VITEK 2 Compact system to ensure this diagnosis. (31) isolates were identified as Candida spp., including (21) (C. albicans (14), C. glabrata (1), C. guilliermondii (2), C. dubliniensis (3), C. parapsilosis (1)) were isolated from oral swabs and (10) isolates includedC. parapsilosis (4), C. albicans (6) were isolated from vaginal swabs. Moreover, these isolates had been detected to have some virulence factors, including phospholipase, esterase, proteinase, coagulase, hemolysin, and biofilm formation. Different species of Candida were isolated and identified from oral and vaginal. Phospholipase (Pz), Esterase (Ez), and Proteinase (Prz) were produced by 19 (61.29%), 16 (51.61%), and 26(83.87%), respectively, out of 31 isolates, whereas. All isolates produce coagulase enzyme except C. dubliniensis, which did not produce coagulase enzyme. All Candida spp. isolates produce hemolysin and biofilm formation in different percentages.
کلیدواژه‌ها
Yeast-Like Fungi؛ Unicellular Microorganisms؛ Deuteromycetes
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مراجع
  1. Rudenko P, Vatnikov Y, Sachivkina N, Rudenko A, Kulikov E, Lutsay V, et al. Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections. Pathogens. 2021;10(6).
  2. Ciurea CN, Kosovski IB, Mare AD, Toma F, Pintea-Simon IA, Man A. Candida and Candidiasis-Opportunism Versus Pathogenicity: A Review of the Virulence Traits. Microorganisms. 2020;8(6).
  3. Deorukhkar SC, Saini S, Mathew S. Non-albicans Candida Infection: An Emerging Threat. Interdiscip Perspect Infect Dis. 2014;2014:615958.
  4. Girish Kumar CP, Menon T, Sundararajan T, Nalini S, Thirunarayan MA, Rajasekaran S, et al. Actividad esterasa en especies de Candida procedentes de pacientes inmunodeficientes. Rev Iberoam Mico. 2006;23(2):101-3.
  5. Silva S, Negri M, Henriques M, Oliveira R, Williams DW, Azeredo J. Candida glabrata, Candida parapsilosis and Candida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance. FEMS Microbiol Rev. 2012;36(2):288-305.
  6. Al-Oebady MAH. Isolation and identification of Candida species from vaginal, urine and oral swabs by chromagar Candida. Int J Adv Res. 2015;3(1):948-56.
  7. Montes K, Ortiz B, Galindo C, Figueroa I, Braham S, Fontecha G. Identification of Candida Species from Clinical Samples in a Honduran Tertiary Hospital. 2019;8(4):237.
  8. Matare T, Nziramasanga P, Gwanzura L, Robertson V. Experimental Germ Tube Induction in Candida albicans: An Evaluation of the Effect of Sodium Bicarbonate on Morphogenesis and Comparison with Pooled Human Serum. Biomed Res Int. 2017;2017:1976273.
  9. Yin P, Peter A, Franken H, Zhao X, Neukamm SS, Rosenbaum L, et al. Preanalytical aspects and sample quality assessment in metabolomics studies of human blood. Clin Chem. 2013;59(5):833-45.
  10. Mariano PdLS, Gonçalves RB, Höfling JF. Storage procedures for yeast preservation: phenotypic and genotypic evaluation. Ann Microbiol. 2007;57(3):461-5.
  11. Fule SR, Das D, Fule RP. Detection of phospholipase activity of Candida albicans and non albicans isolated from women of reproductive age with vulvovaginal candidiasis in rural area. Indian J Med Microbiol. 2015;33(1):92-5.
  12. Tsang CSP, Chu FCS, Leung WK, Jin LJ, Samaranayake LP, Siu SC. Phospholipase, proteinase and haemolytic activities of Candida albicans isolated from oral cavities of patients with type 2 diabetes mellitus. J Med Microbiol. 2007;56(Pt 10):1393-8.
  13. Lan D, Hou S, Yang N, Whiteley C, Yang B, Wang Y. Optimal production and biochemical properties of a lipase from Candida albicans. Int J Mol Sci. 2011;12(10):7216-37.
  14. Yigit N, Aktas E, Dagistan S, Ayyildiz A. Investigating biofilm production, coagulase and hemolytic activity in Candida species isolated from denture stomatitis patients. Eurasian J Med. 2011;43(1):27-32.
  15. Riceto EB, Menezes Rde P, Penatti MP, Pedroso Rdos S. Enzymatic and hemolytic activity in different Candida species. Rev Iberoam Micol. 2015;32(2):79-82.
  16. Lee JH, Kim YG, Cho MH, Lee J. ZnO nanoparticles inhibit Pseudomonas aeruginosa biofilm formation and virulence factor production. Microbiol Res. 2014;169(12):888-96.
  17. Deepa K, Jeevitha T, Michael A. In vitro evaluation of virulence factors of Candida species isolated from oral cavity. J Microbiol Antimicrob. 2015;7(3):28-32.
  18. Dhanasekaran D, Vinothini K, Latha S, Thajuddin N, Panneerselvam A. Human dental biofilm: Screening, characterization, in vitro biofilm formation and antifungal resistance of Candida spp. Saudi J Dent Res. 2014;5(1):55-70.
  19. Hospenthal DR, Beckius ML, Floyd KL, Horvath LL, Murray CK. Presumptive identification of Candida species other than C. albicans, C. krusei, and C. tropicalis with the chromogenic medium CHROMagar Candida. Ann Clin Microbiol Antimicrob. 2006;5:1.
  20. Francis R, Joy N, Sivadas A. Relevance of natural degradable polymers in the biomedical field. Biomed Appl Polym Mater Compos. 2016:303-60.
  21. Kalaiarasan K, Singh R, Chaturvedula L. Changing virulence factors among vaginal non-albicans Candida species. Indian J Med Microbiol. 2018;36(3):364-8.
  22. Maurya IK, Thota CK, Sharma J, Tupe SG, Chaudhary P, Singh MK, et al. Mechanism of action of novel synthetic dodecapeptides against Candida albicans. Biochim Biophys Acta. 2013;1830(11):5193-203.
  23. El-Houssaini HH, Elnabawy OM, Nasser HA, Elkhatib WF. Correlation between antifungal resistance and virulence factors in Candida albicans recovered from vaginal specimens. Microb Pathog. 2019;128:13-9.
  24. de Paula Menezes R, de Melo Riceto EB, Borges AS, de Brito Roder DV, dos Santos Pedroso R. Evaluation of virulence factors of Candida albicans isolated from HIV-positive individuals using HAART. Arch Oral Biol. 2016;66:61-5.
  25. Udayalaxmi J, Shenoy N. Comparison Between Biofilm Production, Phospholipase and Haemolytic Activity of Different Species of Candida Isolated from Dental Caries Lesions in Children. J Clin Diagn Res. 2016;10(4):DC21-3.
  26. Abi-Chacra EA, Souza LO, Cruz LP, Braga-Silva LA, Goncalves DS, Sodre CL, et al. Phenotypical properties associated with virulence from clinical isolates belonging to the Candida parapsilosis complex. FEMS Yeast Res. 2013;13(8):831-48.
  27. Pakshir K, Zomorodian K, Karamitalab M, Jafari M, Taraz H, Ebrahimi H. Phospholipase, esterase and hemolytic activities of Candida spp. isolated from onychomycosis and oral lichen planus lesions. J Mycol Med. 2013;23(2):113-8.
  28. Naglik JR, Challacombe SJ, Hube B. Candida albicans secreted aspartyl proteinases in virulence and pathogenesis. Microbiol Mol Biol Rev. 2003;67(3):400-28.
  29. Neji S, Hadrich I, Trabelsi H, Abbes S, Cheikhrouhou F, Sellami H, et al. Virulence factors, antifungal susceptibility and molecular mechanisms of azole resistance among Candida parapsilosis complex isolates recovered from clinical specimens. J Biomed Sci. 2017;24(1):67.
  1. Yigit N, Aktas A, Ayyildiz A. Detection of coagulase activity in pathogenic Candida species. Int J Med Res. 2008;36(6):1378-82.
  2. Rodrigues CF, Correia A, Vilanova M, Henriques M. Inflammatory Cell Recruitment in Candida glabrata Biofilm Cell-Infected Mice Receiving Antifungal Chemotherapy. J Clin Med. 2019;8(2).
  3. Rodrigues CF, Rodrigues ME, Henriques M. Candida sp. Infections in Patients with Diabetes Mellitus. J Clin Med. 2019;8(1).
  4. Ribeiro FC, Rossoni RD, de Barros PP, Santos JD, Fugisaki LRO, Leao MPV, et al. Action mechanisms of probiotics on Candida spp. and candidiasis prevention: an update. J Appl Microbiol. 2020;129(2):175-85.
  5. Silva S, Henriques M, Martins A, Oliveira R, Williams D, Azeredo J. Biofilms of non-Candida albicans Candida species: quantification, structure and matrix composition. Med Mycol. 2009;47(7):681-9.
  6. Paiva LC, Vidigal PG, Donatti L, Svidzinski TI, Consolaro ME. Assessment of in vitro biofilm formation by Candida species isolates from vulvovaginal candidiasis and ultrastructural characteristics. Micron. 2012;43(2-3):497-502.
  7. Tellapragada C, Eshwara VK, Johar R, Shaw T, Malik N, Bhat PV, et al. Antifungal susceptibility patterns, in vitro production of virulence factors, and evaluation of diagnostic modalities for the speciation of pathogenic Candida from blood stream infections and vulvovaginal candidiasis. J Pathog. 2014;2014:142864.
 

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