Marrubium vulgare (horehound) has been used to treat gastrointestinal disorders, inflammation. This plant has beneficial therapeutic properties and purifies the blood. In this research the changes in the composition of essential oils, aerial herb yield, and antioxidant activity of the aerial parts of Marrubium vulgare were determined at different treatments of zinc. An experiment was performed using completely randomized design (CRD) with five different treatments (T1= Control, T2= Zn sulfate, T3=Nano Zn, T4= EDTA- chelated Zn and T5= Citrate chelated- Zn) in three replications in Eram Garden greenhouse, Shiraz, Iran. The essential oil of air-dried samples was obtained by hydro distillation and analysed by gas chromatography/mass spectrometry (GC/MS). The result showed a significant difference between all zinc treatments for Estragol (methyl chavicol), biological activities and introducing new monoterpene oxygenated. The highest value of methyl chavicol was observed in zinc citrate (68.2%), followed by EDTA chelated Zn (51.2%), Zn sulfate (40.6%) and Nano Zn (18.5%), respectively. The highest essential oil (EO) content (w/w%) was obtained in the plants treated with Nano Zn (0.25%). The high antioxidant activity (735.3 mg/L) was found when Nano-Zn was applied. Results revealed that application of Zn to horehound may be very useful for the production of active natural compounds such as methyl chavicol for drug industries and medical materials. Abbreviations: DPPH: (2,2-diphenyl-1-picryl-hydrazyl-hydrate), EO: Essential oil, GC/MS: Gas chromatography/ mass spectrometry, HEO: Horehound essential oil. |
- Hmamou D.B., Salghi R., Zarrouk A., Zarrok H., Benali O., Errami M., et al. Inhibition effect of horehound (Marrubium vulgare L.) extract towards C38 steel corrosion in HCl solution. Res Chem Intermed. 2013;39(7):3291–302.
- Yabrir B. Essential oil of marrubium vulgare: Chemical composition and biological activities. A review. Nat Prod Sci. 2019;25(2):81–91.
- Asadipour A., Mehrabani M., Nazeri V., Tabarraii M. Composition of the essential oil of Marrubium vulgare L. Pharm Sci. 2005;2:75–82.
- Said-Al Ahl H., Sabra A.S. Growth, herb and essential oil of Marrubium vulgare as affected by phenological stages and planting dates. J chem pharm Res. 2016;8:863–72.
- Burducea M., Zheljazkov VD, Dincheva I, Lobiuc A, Teliban G-C, Stoleru V. Fertilization modifies the essential oil and physiology of basil varieties. Ind Crops Prod. 2018;121:282–93.
- Abugassa I.O., Bashir A.T., Doubali K., Etwir R.H., Abu-Enawel M., Abugassa SO. Characterization of trace elements in medicinal herbs by instrumental neutron activation analysis. J. Radioanal. Nucl. Chem. 2008;278(3):559–63.
- Murch S.J., Haq K., Rupasinghe H.P.V., Saxena P.K. Nickel contamination affects growth and secondary metabolite composition of Hypericum perforatum L. Environ. Exp. Bot. 2003;49(3):251–7.
- Grejtovsky A., Markusova K., Eliasova A., Safarik P.J. The response of chamomile (Matricaria chamomilla L.) plants to soil zinc supply. Plant Soil Environ. 2006;52(1):1–7.
- Bai B.A., Malakouti M.J. Effects of foliar applications of nitrogen, boron, and zinc on fruit set and some quality of almonds. Pajouhesh and Sazandegi. 2005; 8(3):32-40.
- Malakuti M.J., Tehrani M. The role of micronutrients on yield and quality of crops. Tarbiat modares press; 1999.
- Kannan S. Foliar fertilization for sustainable crop production. In: Genetic engineering, biofertilisation, soil quality and organic farming. Springer. 2010; p. 371–402.
- Nelson D.W., Sommers L. Total carbon, organic carbon, and organic matter. Methods of soil analysis: Part 2 chemical and microbiological properties. 1983;9:539–79.
- Bremner J.M. Nitrogen‐total. Methods of soil analysis: Part 3 Chemical methods. 1996;5:1085–121.
- Najafian S., Zahedifar M. Antioxidant activity and essential oil composition of Satureja hortensis L. as influenced by sulfur fertilizer. J. Sci. Food Agric. 2015;95(12):2404–8.
- Pharmacopoeia B. British pharmacopoeia. 2016.
- Adams R.P. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th Edition. Illinois, USA: Allured Publishing Corporation, Carol Stream. 2007;804-6.
- Burits M., Asres K., Bucar F. The antioxidant activity of the essential oils of Artemisia afra, Artemisia abyssinica and Juniperus procera. Phytother Res. 2001; 15: 103-108.
- El-Sawi S.A., Mohamed M.A. Cumin herb as a new source of essential oils and its response to foliar spray with some micro-elements. Food Chem. 2002;77(1):75–80.
- Bisht M., Pande C., Tewari G., Bhatt S., Triphati S. Effect of Zinc on the Growth and Essential Oil Composition of Ocimum gratissimum L. J Essent Oil-Bearing Plants. 2019;22(2):441–54.
- Moghimipour Z., Sourestani M.M., Ansari N.A., Ramezani Z. The effect of foliar application of zinc on essential oil content and composition of holy basil [Ocimum sanctum] at first and second harvests. J Essent Oil-Bearing Plants. 2017;20(2):449–58.
- Marschner H., Cakmak I. High light intensity enhances chlorosis and necrosis in leaves of zinc, potassium, and magnesium deficient bean (Phaseolus vulgaris) plants. J. Plant Physiol. 1989;134(3):308–15.
- Yıldırım A., Mavi A., Kara A.A. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J. Agric Food Chem. 2001;49(8):4083–9.
- Kadri A., Zarai Z., Békir A., Gharsallah N., Damak M., Gdoura R. Chemical composition and antioxidant activity of Marrubium vulgare L. essential oil from Tunisia. Afr j biotechnol.. 2011;10(19):3908–14.
- Said-Al Ahl HAH., Gendy A.SH., Mahmoud A.A., Mohamed H.F.Y. Essential oil composition of Marrubium vulgare L. cultivated in Egypt. Int J Plant Sci. 2015;1(4):138–41.
- Salama M.M., Taher E.E., El-Bahy M.M. Atividades moluscicida e mosquitocida de oleos essenciais de Thymus capitatus Hoff. et Link. e de Marrubium vulgare L. Revista do Instituto de Medicina Tropical de Sao Paulo. 2012;54(5):281-6.
- Hamdaoui B., Wannes W.A., Marrakchi M., Brahim N Ben., Marzouk B. Essential oil composition of two Tunisian horehound species: Marrubium vulgare L. and Marrubium aschersonii Magnus. J Essent Oil-Bearing Plants. 2013;16(5):608-12.
- Srivastava N.K., Sharma S., Misra A. Influence of Zn on allocation of leaf-assimilated 14CO2 into primary metabolites in relation to production of essential oil and curcumin in Turmeric (Curcuma longa L.). World J Agric Sci. 2006;2(2):201-7.
- Coolong T.W., Randle W.M., Toler H.D., Sams C.E. Zinc availability in hydroponic culture influences glucosinolate concentrations in Brassica rapa. Hort Sci. 2004;39(1):84-6.
- Kabata-Pendias A. Trace Elements in Soils and Plants. Boca Raton, FL, Crc Press. 2010; p. 548.
- Pande P., Anwar M., Chand S., Yadav V.K., Patra D.D. Optimal level of iron and zinc in relation to its influence on herb yield and production of essential oil in menthol mint. Commun. Soil Sci Plant Anal. 2007;38(5–6):561-78.
- Najafian S., Zahedifar M. Productivity, essential oil components and herbage yield, of sweet basil as a function of biochar and potassium-nano chelate. J Essent Oil-Bearing Plants. 2018;21(4):886-94.
- Moghaddam M., Alymanesh M.R., Mehdizadeh L., Mirzaei H., Ghasemi Pirbalouti A. Chemical composition and antibacterial activity of essential oil of Ocimum ciliatum, as a new source of methyl chavicol, against ten phytopathogens. Ind Crops Prod. 2014;59:144-8.
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