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Mohammed Aboud, A, Kareem Luaibi, O. (1402). First Molecular Investigation of lumpy Skin Disease in Buffaloes Using the Qualitative and Quantitative Polymerase Chain Reaction Assays, Iraq. سامانه مدیریت نشریات علمی, 78(2), 505-513. doi: 10.22092/ari.2022.359034.2359
A Mohammed Aboud; O Kareem Luaibi. "First Molecular Investigation of lumpy Skin Disease in Buffaloes Using the Qualitative and Quantitative Polymerase Chain Reaction Assays, Iraq". سامانه مدیریت نشریات علمی, 78, 2, 1402, 505-513. doi: 10.22092/ari.2022.359034.2359
Mohammed Aboud, A, Kareem Luaibi, O. (1402). 'First Molecular Investigation of lumpy Skin Disease in Buffaloes Using the Qualitative and Quantitative Polymerase Chain Reaction Assays, Iraq', سامانه مدیریت نشریات علمی, 78(2), pp. 505-513. doi: 10.22092/ari.2022.359034.2359
Mohammed Aboud, A, Kareem Luaibi, O. First Molecular Investigation of lumpy Skin Disease in Buffaloes Using the Qualitative and Quantitative Polymerase Chain Reaction Assays, Iraq. سامانه مدیریت نشریات علمی, 1402; 78(2): 505-513. doi: 10.22092/ari.2022.359034.2359

First Molecular Investigation of lumpy Skin Disease in Buffaloes Using the Qualitative and Quantitative Polymerase Chain Reaction Assays, Iraq

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مقاله 2، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 505-513    اصل مقاله (603.1 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359034.2359
نویسندگان
A Mohammed Aboud* ؛ O Kareem Luaibi
Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
چکیده
Lumpy skin disease (LSD) is one of the most important infectious bovine diseases in Iraq in the last 10 years; however, the current study represents the first investigation to confirm the disease in buffaloes as well as ticks with estimation the association of positivity to clinical vital signs and risk factors.  A total of 150 buffaloes were subjected for blood sampling, skin lesions and ticks. All the collected samples; 150 blood, 13 skin lesions, and 29 tick samples, were examined molecularly using the conventional and real-time PCR assays. The total positive results of blood, skin and ticks by conventional PCR were 5.33, 7.69 and 0%, respectively; while for real-time PCR, it was 15.33, 7.69 and, 0%, respectively. Insignificant differences were showed between values of temperature, pulse and respiratory rates of LSD positive and negative buffaloes by the conventional and real-time PCR assays. The association of positive conventional PCR results to risk factors (age, sex and region) was revealed a significant increase in prevalence and risk of LSD in buffaloes aged < 1 year; but for gender, insignificant variation in prevalence but not risk was seen between females and males. In case of different geographical region, significant higher prevalence was reported in Wasit; while, buffaloes of Maysan and Wasit were appeared at higher risk than those of Dhi-Qar. Regarding real-time PCR, insignificant differences were found between values of < 1, 1-4 and > 4-8 years age old, but not in group of >8 that showed a significant decline in positivity (0%). For sex, insignificant variation in prevalence, but not risk, was seen between females and males. Concerning region, buffaloes of Wasit province were recorded a significant higher values of prevalence and risk than other regions. LSD in buffaloes is mainly sub-acute, and PCR appeared to be a suitable diagnostic method in detection of infection; however, furthermore studies are necessary.
کلیدواژه‌ها
Real-Time PCR؛ Envelop Protein (P32) Gene؛ Blood؛ Skin Lesion؛ Ticks
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مراجع
  1. Constable PD, Hinchcliff KW, Done SH, Grünberg W. Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs, and Goats: Elsevier; 2017.
  2. Al-sabawy HB, AL-Hamdany EK, AL-Sultan AA, RDAM SA. A High Light On Lumpy Skin Disease In Iraq And The Middle East: A Review Article. J Appl Vet Sci. 2020;5(2):94-103.
  3. Al-Salihi KA, Hassan IQ. Lumpy Skin Disease in Iraq: Study of the Disease Emergence. Transbound Emerg Dis. 2015;62(5):457-62.
  4. Abdulqa HY, Rahman HS, Dyary HO, Othman HH. Lumpy skin disease. Reprod Immunol. 2016;1(4):25-31.
  5. Tuppurainen ES, Oura CA. Review: lumpy skin disease: an emerging threat to Europe, the Middle East and Asia. Transbound Emerg Dis. 2012;59(1):40-8.
  6. Gharban HAJ, Al-Shaeli SJJ, Al-Fattli HHH, Altaee MNK. Molecular and histopathological confirmation of clinically diagnosed lumpy skin disease in cattle, Baghdad Province of Iraq. Vet World. 2019;12(11):1826-32.
  7. Sevik M, Dogan M. Epidemiological and Molecular Studies on Lumpy Skin Disease Outbreaks in Turkey during 2014-2015. Transbound Emerg Dis. 2017;64(4):1268-79.
  8. Abutarbush SM. Lumpy Skin Disease (Knopvelsiekte, Pseudo-Urticaria, Neethling Virus Disease, Exanthema Nodularis Bovis). In: Bayry J, editor. Emerging and Re-emerging Infectious Diseases of Livestock. Cham: Springer International Publishing; 2017. p. 309-26.
  9. Sprygin A, Pestova Y, Wallace DB, Tuppurainen E, Kononov AV. Transmission of lumpy skin disease virus: A short review. Virus Res. 2019;269:197637.
  10. Gharban HAJ, Yousif AA. Serological and Molecular Phylogenetic Detection of Coxiella burnetii in Lactating Cows, Iraq. Iraqi J Vet Med. 2020;44:42-50.
  11. Ochwo S, VanderWaal K, Ndekezi C, Nkamwesiga J, Munsey A, Witto SG, et al. Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreaks in Uganda 2017-2018. BMC Vet Res. 2020;16(1):66.
  12. Sudhakar SB, Mishra N, Kalaiyarasu S, Jhade SK, Hemadri D, Sood R, et al. Lumpy skin disease (LSD) outbreaks in cattle in Odisha state, India in August 2019: Epidemiological features and molecular studies. Transbound Emerg Dis. 2020;67(6):2408-22.
  13. Molla W, Frankena K, Gari G, Kidane M, Shegu D, de Jong MCM. Seroprevalence and risk factors of lumpy skin disease in Ethiopia. Prev Vet Med. 2018;160:99-104.
  14. Fagbo S, Coetzer JA, Venter EH. Seroprevalence of Rift Valley fever and lumpy skin disease in African buffalo (Syncerus caffer) in the Kruger National Park and Hluhluwe-iMfolozi Park, South Africa. J S Afr Vet Assoc. 2014;85(1):e1-e7.
  15. Elhaig MM, Selim A, Mahmoud M. Lumpy skin disease in cattle: Frequency of occurrence in a dairy farm and a preliminary assessment of its possible impact on Egyptian buffaloes. Onderstepoort J Vet Res. 2017;84(1):e1-e6.
  16. Faris DN, El-Bayoumi K, El-Tarabany M, Kamel ER. Prevalence and risk factors for lumpy skin disease in cattle and buffalo under subtropical environmental conditions. Adv Anim Vet Sci. 2021;9(9):1311-6.
  17. Caliendo AM, Schuurman R, Yen-Lieberman B, Spector SA, Andersen J, Manjiry R, et al. Comparison of quantitative and qualitative PCR assays for cytomegalovirus DNA in plasma. J Clin Microbiol. 2001;39(4):1334-8.
  1. Houghton SG, Cockerill FR. Real-time PCR: overview and applications. Surgery. 2006;139(1):1-5.
  2. Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonak J, Lind K, et al. The real-time polymerase chain reaction. Mol Aspects Med. 2006;27(2-3):95-125.
  3. Das M, Chowdhury MSR, Akter S, Mondal AK, Jamal M. An updated review on lumpy skin disease: perspective of Southeast Asian countries. J Adv Biotechnol Exp Ther. 2021;4:322-33.
  4. Ayelet G, Haftu R, Jemberie S, Belay A, Gelaye E, Sibhat B, et al. Lumpy skin disease in cattle in central Ethiopia: outbreak investigation and isolation and molecular detection of the virus. Rev Sci Tech. 2014;33(3):877-87.
  5. Abd Elmohsen M, Selim A, Abd Elmoneim AE. Prevalence and molecular characterization of Lumpy Skin Disease in cattle. Benha Vet Med J. 2019;37(1):144-7.
  6. Mhemid KM. Molecular detection of lumpy skin disease virus in cattle by polymerase chain reaction in Iraq: Khitam Mahdi Mhemid and Ibtesam Qasim Hassan. Iraqi J Vet Med. 2016;40(1):83-8.
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