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M. Sulaiman, Asmaa, A. Hussein, Suha, I. Husain, Vian. (1401). Detection of antibiotic resistance genes (CTX-M, Van A & Van B) of Enterococcus faecalis isolated from children with bacteremia by RT-PCR. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2022.359148.2380
Asmaa M. Sulaiman; Suha A. Hussein; Vian I. Husain. "Detection of antibiotic resistance genes (CTX-M, Van A & Van B) of Enterococcus faecalis isolated from children with bacteremia by RT-PCR". سامانه مدیریت نشریات علمی, , , 1401, -. doi: 10.22092/ari.2022.359148.2380
M. Sulaiman, Asmaa, A. Hussein, Suha, I. Husain, Vian. (1401). 'Detection of antibiotic resistance genes (CTX-M, Van A & Van B) of Enterococcus faecalis isolated from children with bacteremia by RT-PCR', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2022.359148.2380
M. Sulaiman, Asmaa, A. Hussein, Suha, I. Husain, Vian. Detection of antibiotic resistance genes (CTX-M, Van A & Van B) of Enterococcus faecalis isolated from children with bacteremia by RT-PCR. سامانه مدیریت نشریات علمی, 1401; (): -. doi: 10.22092/ari.2022.359148.2380

Detection of antibiotic resistance genes (CTX-M, Van A & Van B) of Enterococcus faecalis isolated from children with bacteremia by RT-PCR

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 19 تیر 1401  XML
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359148.2380
نویسندگان
Asmaa M. Sulaiman email 1؛ Suha A. Hussein2؛ Vian I. Husain1
1Northern Technical university, College of Health and Medical Techniques Medical Lab, Techniques department, Iraq
2University of Sulaimani, College of Dentistry, Basic Sciences, Iraq
چکیده
Fever is one of the most common diseases that affect humans, as it is the result of any disease or development and worsening of the disease for most people with general infections in the body. Therefore, the aim of this study was to evaluate antibiotic resistance genes (CTX-M, Van A & Van B) of Enterococcus faecalis isolated from children with bacteremia by RT-PCR. A total of 200 child was enrolled in the study, 100 child with fever and 100 healthy child (not suffering from any problem), that is, they are a control group for detection of antibiotic resistance genes (CTX-M, Van A&B) of Enterococcus faecalis by RT-PCR. The age of the two groups ranged from one to five years. Four ml of venous blood sample was collected from each child; the venipuncture area was sterilized first with alcohol at a rate of 70%, followed by medical iodine and then sterilized with alcohol again to avoid contamination with skin flora. The blood samples were cultured on media for isolating bacteria. Then, the resistant isolates of E. faecalis to vancomycin and cefotaxime antibiotic were taken and kept in special nutrient agar media where the DNA of the bacteria was extracted using (Zymogene Extraction kit, Japan). The detection of the exact genes (CTX-M, Van A&B) was done using Real Time PCR technology according to the protocol mentioned by the company (Sacace biotechnology, Italy). The study presented that, 40% of children with fever have positive blood culture compared with 5% in the control group with a significant difference between the two groups (P<0.001). The study found that, 32.5% of bacteremic children were due S. aureus, 30%, 5%, 4% was due to E. faecalis, E. coli, P. aeruginosa and Klebsiella spp, respectively with significant difference (P<0.01).
کلیدواژه‌ها
Antibiotic resistance؛ CTX-M؛ Vancomycin؛ Enterococcus faecalis RT-PCR
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تعداد مشاهده مقاله: 27


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