The high requirement of broiler to protein in the diet need more knowledge about protein quality, availability of amino acids, prices or cost, minimum concentration of anti-nutritional factor (ANFs) and gastrointestinal tract (GIT) health. Azolla is a floating fern with highly protein content and vitamins (Vit B12, Vit A, and growth promoters), essential amino acids and minerals (phosphor, calcium, ferrous and potassium). Azolla can fixes nitrogen from atmosphere by the presence of the blue-green algae (Anabaena azollae) in their leaves cavities. This study was investigating the physiological and production effect of inclusion different levels of azolla in broiler feed. 400 broiler Ross chicks were randomly divided into four equal groups (n=100). Control group received normal diet without any supplementation. Animals in treatment 1 group (T1) received 15% azolla in their normal diet formulation, treatment 2 group (T2) received 30% azolla in their diet formulation, and treatment 3 group (T3) received 45% azolla. The animals had ad libitum access to food and water. Body weights and food conversion ratio (FCR) were monitored weekly and blood samples collected at the end of the experiment for assessment of lipid profile (cholesterol, triglyceride, HDL, LDL and VLDL), blood proteins (total protein and albumin) and activity of liver enzymes (GOT, GPT and ALP). The results showed increase body weight significantly (p≤0.5) in T3 group compared with control along with significant decrease in FCR at a same quantity of feed for each group (3 Kg). The results showed no change in physiological parameters (lipid profile, blood protein and liver enzymes activity) except for the levels of GOT which was decrease significantly (p≤0.5). In conclusion, the inclusion of azolla at 15, 30 and 45% in broiler feed has a good effect on body weight and FCR, with no adverse effect on normal physiology of broilers. Also, supplementation of Azolla can decrease production cost in the broiler industry with more than 30%. |