اخبار و اعلانات

 آمار

تعداد نشریات283
تعداد نشریات سازمان81
تعداد شماره‌ها2,722
تعداد مقالات31,022
تعداد مشاهده مقاله27,177,489
تعداد دریافت فایل اصل مقاله16,170,030
Kareem Kadhim, D, Abdulsalam Hraija, B, Aqeele, G. (1401). Molecular-Genotyping Detection of Entamoeba histolytica in Diarrheic Patients. سامانه مدیریت نشریات علمی, 78(1), 337-343. doi: 10.22092/ari.2022.358947.2336
D Kareem Kadhim; B Abdulsalam Hraija; G Aqeele. "Molecular-Genotyping Detection of Entamoeba histolytica in Diarrheic Patients". سامانه مدیریت نشریات علمی, 78, 1, 1401, 337-343. doi: 10.22092/ari.2022.358947.2336
Kareem Kadhim, D, Abdulsalam Hraija, B, Aqeele, G. (1401). 'Molecular-Genotyping Detection of Entamoeba histolytica in Diarrheic Patients', سامانه مدیریت نشریات علمی, 78(1), pp. 337-343. doi: 10.22092/ari.2022.358947.2336
Kareem Kadhim, D, Abdulsalam Hraija, B, Aqeele, G. Molecular-Genotyping Detection of Entamoeba histolytica in Diarrheic Patients. سامانه مدیریت نشریات علمی, 1401; 78(1): 337-343. doi: 10.22092/ari.2022.358947.2336

Molecular-Genotyping Detection of Entamoeba histolytica in Diarrheic Patients

Archives of Razi Institute
مقاله 42، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 337-343    اصل مقاله (626.69 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358947.2336
نویسندگان
D Kareem Kadhim* 1؛ B Abdulsalam Hraija2؛ G Aqeele2
1Department of Anatomy and Biology, College of Medicine, University of Wasit, Wasit, Iraq
2Department of Microbiology, College of Medicine, University of Wasit, Wasit, Iraq
چکیده
Amebiasis is caused by Entamoeba histolytica, a protozoan that is found worldwide. The degree of pathogenesis of clinical isolates varies greatly. This study was aimed to molecular identification of E. histolytica in children using the nested polymerase chain reaction (nPCR), and then, a genotyping of positive E. histolytica isolates using the quantitative PCR (qPCR) assay through targeting serine-rich E. histolytica protein (SREHP) gene. For this purpose, a total of 50 bloody diarrheic stool samples were collected from the children attended to the Al-Zahraa’ Teaching Hospital and Alkut Hospital for Gynecology, Obstetric and Pediatrics (Alkut, Wasit, Iraq) were subjected to the present study from September to December 2021. Firstly, the extracted DNAs that amplified using specific primers through targeting 18S rRNA gene and tested using nPCR assay were revealed an overall 48% (24/50) positive samples for E. histolytica. For genotyping, our results were detected an existence of four different genotypes (I, II, III and IV) with a significant prevalence of Genotype-II (54.17%) when compared to Genotype-I (20.83%), Genotype-III (12.5%) and Genotype- IV (12.5%). In addition, results of melting temperature of targeted genotypes were 84ºC, 83 - 83.5ºC, 82.5ºC and 81ºC for Genotype-I, II, III and IV, respectively. In conclusion, molecular amplification of 18S rRNA gene was revealed the large prevalence of E. histolytica among bloody diarrheic children of study areas; while, amplification of SREHP gene was reflected the widespread phenotypic variation of the Genotype-II suggesting the high ability of this genotype to spread infection in children. In different endemic areas as Iraq, the utilization of high-resolution genotyping methods showed the extremely polymorphic genetic structure of this parasite.
کلیدواژه‌ها
Entamoeba histolytica؛ genotyping؛ 18S rRNA؛ SREHP gene؛ Iraq
سایر فایل های مرتبط با مقاله
مراجع
  1. Rawat A, Singh P, Jyoti A, Kaushik S, Srivastava VK, Design D. Averting transmission: A pivotal target to manage amoebiasis. Chem Biol Drug Des. 2020;96(2):731-44.
  2. Cimino RO, Jeun R, Juarez M, Cajal PS, Vargas P, Echazú A, et al. Identification of human intestinal parasites affecting an asymptomatic peri-urban Argentinian population using multi-parallel quantitative real-time polymerase chain reaction. Parasit Vectors. 2015;8(1):1-7.
  3. Begum S, Gorman H, Chadha A, Chadee K. Role of inflammasomes in innate host defense against Entamoeba histolytica. J Leukoc Biol. 2020;108(3):801-12.
  4. Shirley D-AT, Farr L, Watanabe K, Moonah S, editors. A review of the global burden, new diagnostics, and current therapeutics for amebiasis. Open forum infectious diseases; 2018: Oxford University Press US.
  5. Kantor M, Abrantes A, Estevez A, Schiller A, Torrent J, Gascon J, et al. Entamoeba histolytica: updates in clinical manifestation, pathogenesis, and vaccine development. Can J Gastroenterol Hepatol. 2018;2018.
  6. Bercu TE, Petri WA, Behm BW. Amebic colitis: new insights into pathogenesis and treatment. Curr Gastroenterol Rep. 2007;9(5):429-33.
  7. Aguilar-Rojas A, Olivo-Marin J-C, Guillen N. The motility of Entamoeba histolytica: finding ways to understand intestinal amoebiasis. Curr Opin Microbiol. 2016;34:24-30.
  8. Greene JM, Collins F, Lefkowitz EJ, Roos D, Scheuermann RH, Sobral B, et al. National Institute of Allergy and Infectious Diseases bioinformatics resource centers: new assets for pathogen informatics. Infect. Immun. 2007;75(7):3212-9.
  9. Laude A, Valot S, Desoubeaux G, Argy N, Nourrisson C, Pomares C, et al. Is real-time PCR-based diagnosis similar in performance to routine parasitological examination for the identification of Giardia intestinalis, Cryptosporidium parvum/Cryptosporidium hominis and Entamoeba histolytica from stool samples? Evaluation of a new commercial multiplex PCR assay and literature review. 2016;22(2):190. e1-. e8.
  10. Foo PC, Nurul Najian A, Muhamad NA, Ahamad M, Mohamed M, Yean Yean C, et al. Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample. BMC Biotechnol. 2020;20(1):1-15.
  11. Gupta S, Smith L, Diakiw AJPC. Amebiasis and Amebic Liver Abscess in Children. 2022;69(1):79-97.
  12. Das K, kumar Sardar S, Ghosal A, Saito-Nakano Y, Dutta S, Nozaki T, et al. Multilocus sequence typing (MLST) of Entamoeba histolytica identifies kerp2 as a genetic marker associated with disease outcomes. Parasitol Int. 2021;83:102370.
  13. Uddin MJ, Leslie JL, Petri Jr WA. Host protective mechanisms to intestinal amebiasis. Trends Parasitol. 2021;37(2):165-75.
  14. Al-Ardi M. Illumination on the structure and characteristics of Entamoeba histolytica genome. Al-Qadisiyah J Pure Sci. 2021;26(4):19-26.
  15. Ali IKM, Mondal U, Roy S, Haque R, Petri Jr WA, Clark CG. Evidence for a link between parasite genotype and outcome of infection with Entamoeba histolytica. J Clin Microbiol. 2007;45(2):285-9.
  16. Haque R, Ali I, Akther S, Petri Jr WA. Comparison of PCR, isoenzyme analysis, and antigen detection for diagnosis of Entamoeba histolytica infection. J Clin Microbiol. 1998;36(2):449-52.
  17. Paul J, Srivastava S, Bhattacharya S. Molecular methods for diagnosis of Entamoeba histolytica in a clinical setting: an overview. Exp Parasitol. 2007;116(1):35-43.
  18. Mahmood SAF, Bakr HM. Molecular identification and prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, northern Iraq. Pol J Microbiol.2020;69(3):263.
  19. Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J. PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia. J Clin Microbiol. 2007;45(3):1035-7.
  20. Al-Braiken FA, Salem HS. Diagnosis of Entamoeba histolytica in symptomatic children, Jeddah City, Saudi Arabia. Egypt J Immunol. 2008;15(1):85-92.
  21. Peralta ML, Ayala J. Algunas consideraciones sobre la prevalencia actual de Entamoeba histolytica, Giardia duodenalis, coccidios, microsporidios y mixosporidios en Colombia. Revista Salud Uninorte. 2008;24(2):294-302.
  22. Ngui R, Angal L, Fakhrurrazi SA, Lian YLA, Ling LY, Ibrahim J, et al. Differentiating Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii using nested polymerase chain reaction (PCR) in rural communities in Malaysia. Parasit Vectors. 2012;5(1):1-7.
  23. ElBakri A, Samie A, Ezzedine S, Odeh RA. Differential detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in fecal samples by nested PCR in the United Arab Emirates (UAE). Acta Parasitologica. 2013;58(2):185-90.
  24. Al‐Areeqi MA, Sady H, Al‐Mekhlafi HM, Anuar TS, Al‐Adhroey AH, Atroosh WM, et al. First molecular epidemiology of Entamoeba histolytica, E. dispar and E. moshkovskii infections in Yemen: different species‐specific associated risk factors. Trop Med Int Health. 2017;22(4):493-504.
  25. Yimer M, Zenebe Y, Mulu W, Abera B, Saugar JM. Molecular prevalence of Entamoeba histolytica/dispar infection among patients attending four health centres in north-west Ethiopia. Trop Doct. 2017;47(1):11-5.
  26. Bahrami F, Haghighi A, Zamini G, Khademerfan M. Differential detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in faecal samples using nested multiplex PCR in west of Iran. Epidemiol Infect. 2019;147.
  27. Abozahra R, Mokhles M, Baraka K. Prevalence and Molecular Differentiation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in Egypt. Acta Parasitologica. 2020;65(4):929-35.
  28. Pu L-H, Li Z, Wu J, Zhang Y-L, Chen J-Q, Yang J-F, et al. Prevalence, molecular epidemiology and zoonotic risk of Entamoeba spp. from experimental macaques in Yunnan Province, southwestern China. Epidemiol Infect. 2020;119(8):2733-40.
  29. Ahmed S, Klena J, Albana A, Alhamdani F, Oskoff J, Soliman M, et al. Characterization of human rotaviruses circulating in Iraq in 2008: atypical G8 and high prevalence of P [6] strains. Infect Genet Evol. 2013;16:212-7.
  1. Harb A, Abraham S, Rusdi B, Laird T, O’Dea M, Habib I. Molecular detection and epidemiological features of selected bacterial, viral, and parasitic enteropathogens in stool specimens from children with acute diarrhea in Thi-Qar Governorate, Iraq. Int J Environ Res Public Health. 2019;16(9):1573.
  2. Gilchrist CA, Petri SE, Schneider BN, Reichman DJ, Jiang N, Begum S, et al. Role of the gut microbiota of children in diarrhea due to the protozoan parasite Entamoeba histolytica. J Infect Dis. 2016;213(10):1579-85.
  3. Mero S, Kirveskari J, Antikainen J, Ursing J, Rombo L, Kofoed P-E, et al. Multiplex PCR detection of Cryptosporidium sp, Giardia lamblia and Entamoeba histolytica directly from dried stool samples from Guinea-Bissauan children with diarrhoea. Infect Dis. 2017;49(9):655-63.
  4. Connor BA, Martin GJ, Riddle MS. Use of the multiplex diagnostic PCR panel in diarrheal disease: expert guidance on the interpretation of results with a focus on Travelers' diarrhea. Official J American College Gastroenterol. 2020;115(10):1553-5.
  5. Ayeh-Kumi PF, Ali IM, Lockhart LA, Gilchrist CA, Petri Jr WA, Haque R. Entamoeba histolytica: genetic diversity of clinical isolates from Bangladesh as demonstrated by polymorphisms in the serine-rich gene. Exp Parasitol. 2001;99(2):80-8.
  6. Araz RE, Koru Ö, Tanyüksel M, Özekinci T, Ceylan A, Kilbaş HZG, et al. An investigation of the relationship between clinical features of amoebiasis and Entamoeba histolytica genotypes. Turk J Med Sci. 2012;42(1):1147-56.
آمار
تعداد مشاهده مقاله: 981
تعداد دریافت فایل اصل مقاله: 295


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب