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تأثیر محلول‌پاشی برگی کیتوزان و دیاکسیدتیتانیوم (بالک و نانو) بر عملکرد و پاسخهای بیوشیمیایی اکوتیپ‌های ماریتیغال (Silybum marianum (L.) Gaertn.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 8، دوره 38، شماره 3 - شماره پیاپی 113، مهر 1401، صفحه 450-463 اصل مقاله (733.9 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2022.357559.3126 | ||
| نویسندگان | ||
| سمیرا جعفری1؛ صادق موسوی فرد* 2؛ عبدالحسین رضایی نژاد3؛ حسن مومیوند4؛ کریم سرخه5 | ||
| 1دانشجوی دکتری، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران | ||
| 2استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران | ||
| 3استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران | ||
| 4استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران | ||
| 5استادیار، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| چکیده | ||
| ماریتیغال (Silybum marianum (L. Gaertn.)) گیاهی با کاربردهای متنوع دارویی است که با انواع شرایط آب و هوایی سازگار است. به منظور ارزیابی تأثیر غلظتهای مختلف کیتوزان و دیاکسیدتیتانیوم (بالک و نانو) بر افزایش عملکرد و شاخصهای بیوشیمیایی اکوتیپ‌های مختلف گیاه ماریتیغال، پژوهشی زراعی به‌صورت فاکتوریل در قالب طرح آماری بلوک‌های کامل تصادفی با سه تکرار در دو سال زراعی انجام شد. تیمارهای آزمایشی شامل اکوتیپ‌های ماریتیغال در پنج سطح (بوداکالازی، ساری، خمین، خرمآباد و اهواز) و نوع و غلظت الیسیتور در 9 سطح (محلول‌پاشی با آب مقطر (شاهد)، کیتوزان بالک (50 و 100 میلیگرم در لیتر)، نانو کیتوزان (50 و 100 میلیگرم در لیتر)، دیاکسیدتیتانیوم بالک (50 و 100 میلیگرم در لیتر) و نانو دیاکسیدتیتانیوم (50 و 100 میلیگرم در لیتر)) بود. نتایج نشان داد که محلول‌پاشی کیتوزان و دیاکسید تیتانیوم (نانو و بالک) در افزایش عملکرد گیاه و آنزیمهای دفاعی مؤثر بود. تأثیر محلول‌پاشی کیتوزان و دیاکسید تیتانیوم در فرم نانو روی صفات مذکور نسبت به فرم بالک و شاهد برتری داشت. براساس نتایج مقایسه میانگین‌ها، با محلول‌پاشی نانوکیتوزان در غلظت50 میلیگرم در لیتر، عملکرد بوته افزایش یافت. همچنین، محلول‌پاشی نانوکیتوزان در غلظت 100 میلیگرم در لیتر باعث افزایش فعالیت آنزیمهای دفاعی شد. بهطور کلی، کاربرد نانوکیتوزان در افزایش عملکرد و همچنین بهبود خصوصیات بیوشیمیایی اکوتیپ‌های ماریتیغال بهخصوص اکوتیپ بوداکالازی تأثیرگذار بود که میتواند به‌عنوان راهکار مناسبی برای افزایش عملکرد این گیاه باشد. همچنین استفاده از این اکوتیپ در برنامه های اصلاحی با توجه به عملکرد بالاتر قابل توصیه است. | ||
| کلیدواژه‌ها | ||
| نانوکیتوزان؛ آنزیمهای دفاعی؛ الیسیتور؛ مالوندیآلدئید؛ بوداکالازی | ||
| عنوان مقاله [English] | ||
| Effects of chitosan and titanium dioxide (bulk and nano) foliar application on yield and biochemical responses of Silybum marianum (L. Gaertn.) ecotypes | ||
| نویسندگان [English] | ||
| S. Jafari1؛ S Mousavi-Fard2؛ A. Rezaei Nejad3؛ H. Mumivand4؛ K. Sorkheh5 | ||
| 1Ph.D. student, Department of Horticultural Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran | ||
| 2Department of Horticultural Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran | ||
| 3Department of Horticultural Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran | ||
| 4Department of Horticultural Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran | ||
| 5Department of Production Engineering and Plant Genetics, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran | ||
| چکیده [English] | ||
| Milk thistle (Silybum marianum (L. Gaertn.)) is a plant with various medicinal uses which is adapted to different climates. To investigate the effects of different concentrations of chitosan (CS) and titanium dioxide (TiO2) on increasing yield and biochemical indices of milk thistle ecotypes, a farm factorial experiment was conducted in a randomized complete block design with three replications during two crop years. Experimental treatments included milk thistle ecotype at five levels (Budakalazi, Sari, Khomein, Khorramabad, and Ahvaz) and type and elicitor concentration at nine levels (foliar application of distilled water (control), bulk CS (50 and 100 mg L-1), nano CS (50 and 100 mg L-1), bulk TiO2 (50 and 100 mg L-1), and nano TiO2 (50 and 100 mg L-1)). The results showed that foliar application of CS and TiO2 (bulk and nano) effectively increased yield and defense enzymes. In all these traits, foliar application of CS and TiO2 in nano form was more effective compared to the bulk form and control. Based on the mean comparisons results, the plant yield increased with foliar application of nano CS at the concentration of 50 mg L-1. Also, foliar application of nano CS at the concentration of 100 mg L-1 increased the activity of defense enzymes. In general, foliar application of nano CS increased the yield as well as improved the biochemical traits of milk thistle ecotypes, especially the Budakalazi one, which could be considered as a suitable way to increase the yield of this plant. Considering the higher yield of Budakalazi ecotype, it could be also recommended to be used in breeding programs. | ||
| کلیدواژه‌ها [English] | ||
| Nano chitosan, defense enzymes, elicitor, malondialdehyde, Budakalazi | ||
| مراجع | ||
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