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آنالیز تقریبی و تعیین میزان کیتین در برخی حشرات قابل استفاده در تغذیه طیور | ||
| علوم دامی | ||
| دوره 35، شماره 134، خرداد 1401، صفحه 91-102 اصل مقاله (1.35 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2021.343635.2080 | ||
| نویسندگان | ||
| مهدی امیرصادقی* ؛ امیرحسین علیزاده قمصری | ||
| استادیار مؤسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران. | ||
| چکیده | ||
| امروزه علاقه وافری برای استفاده از حشرات در تغذیه دام و طیور وجود دارد. تمامی حشرات اسکلت خارجی می‌سازند که بر پایه کیتین است. تعیین مقدار دقیق کیتین در حشرات به دلیل کاربرد این ماده در صنایع مختلف و گوارش اندک در اکثر گونه‌های دامی، ضروری است. با توجه به ساختار کیتین و نامحلول بودن آن در حلال‌های رایج، اندازه‌گیری مقدار این ترکیب اغلب دشوار و به صورت تخمینی انجام می‌گیرد. در این پژوهش، دو روش مختلف برای اندازه‌گیری میزان کیتین حشرات، مورد استفاده و مقایسه قرار گرفت. آزمایش با استفاده از شش نمونه مختلف حشره شامل لارو سوسک شبزی (میلورم) (Tenebrio molitor)، جیرجیرک (Gryllodes sigillatus)، زنبور عسل (Apis mellifera meda) و سه واریته 31، 51 و 107 کرم ابریشم (Bombix mori) انجام شد. در روش اول، میزان کیتین با استفاده از اندازه‌گیری فیبر نامحلول در شوینده اسیدی تخمین زده شد. در روش دوم، میزان کیتین با روش خالص‌سازی شیمیایی اندازه‌گیری شد. مقدار کیتین اندازه‌گیری شده با روش خالص‌سازی شیمیایی برای نمونه زنبور عسل، 071/0 ±05/12، میلورم، 259/0±18/8، جیرجیرک، 107/0±79/7 و برای سه واریته کرم ابریشم به ترتیب 058/0±92/7، 091/0 ±93/4 و 116/0 ±08/6 درصد برای واریته های 31، 51 و 107 بود. با توجه به مقدار بیشینه توصیه شده کیتین در خوراک طیور در سطح 7 گرم در کیلوگرم و تاثیر کیتین در محاسبه پروتئین کل، تعیین مقدار کیتین حشرات قبل از مصرف در جیره ضروری است و بر اساس نتایج به دست آمده از این تحقیق، استفاده از روش خالص‌سازی شیمیایی جهت تعیین میزان کیتین حشرات، پیشنهاد می‌شود. | ||
| کلیدواژه‌ها | ||
| حشرات؛ کیتین؛ خالص‌سازی شیمیایی | ||
| عنوان مقاله [English] | ||
| Approximate Analysis and Determination of Chitin Content in Some Insects Used In Poultry Nutrition | ||
| نویسندگان [English] | ||
| Mehdi Amirsadeghi؛ Amir Hossein Alizadeh Ghamsari | ||
| Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO),Karaj, Iran | ||
| چکیده [English] | ||
| Nowadays, there is an abundant interest in the use of insects in poultry and livestock nutrition. All insects produce chitin-based exoskeletons. A precise determination of chitin content of insects is necessary because of its application in various industries and poor digestion in some livestock. Therefore it is necessary to specify their skeleton composition including chitin. Due to the structure of chitin and insolubility in common solvents, the measurement of this compound is difficult and undertaken by estimation. In this study, two different methods are used and compared for chitin content measurement of insects. The experiment was carried out using six different samples of insects including larva of darkling beetle (mealworm) (Tenebrio molitor), crickets (Gryllodes sigillatus), honey bee (Apis mellifera meda) and varieties 31, 51 and 107 of silkworm (Bombix mori). In the first method, chitin content was estimated by measuring the acid detergent fiber. In the second method, the chitin measured by chemical purification method. The measured chitin contents by chemical purification were 12.05%± 0.071 for honey bee, 8.18%± 0.259 for mealworm, 7.79% ± 0.107 for cricket and 7.92% ± 0.058, 4.93% ± 0.091, 6.08% ± 0.116 for variety 31, 51 and 107 of silkworm samples respectively. Due to the recommended maximum amount of chitin in poultry feed at the level of 7g/kg and effect of chitin in determination of total protein, measurement of chitin before usage in feed is necessary and according to the obtained results, for determination of chitin content use of chemical purification method is recommended. | ||
| کلیدواژه‌ها [English] | ||
| Chemical Purification, Chitin, Insects | ||
| مراجع | ||
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