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Dawood Saleem, H, Fawwaz Alfarras, A, Hameed, N. M, Hasan Al-Zubaidi, S, Shnain Ali, M, Hamood, S. A, Hameed, S, Hamad, D. A, Ali Hussein, H, Mohsin Al-Dhalemi, D. (1401). Detection of Salmonella spp. by Traditional and PCR Assays in Raw Milk, Maysan, Iraq. سامانه مدیریت نشریات علمی, 77(4), 1453-1457. doi: 10.22092/ari.2022.359086.2369
H Dawood Saleem; A Fawwaz Alfarras; N. M Hameed; S Hasan Al-Zubaidi; M Shnain Ali; S. A Hamood; S Hameed; D. A Hamad; H Ali Hussein; D Mohsin Al-Dhalemi. "Detection of Salmonella spp. by Traditional and PCR Assays in Raw Milk, Maysan, Iraq". سامانه مدیریت نشریات علمی, 77, 4, 1401, 1453-1457. doi: 10.22092/ari.2022.359086.2369
Dawood Saleem, H, Fawwaz Alfarras, A, Hameed, N. M, Hasan Al-Zubaidi, S, Shnain Ali, M, Hamood, S. A, Hameed, S, Hamad, D. A, Ali Hussein, H, Mohsin Al-Dhalemi, D. (1401). 'Detection of Salmonella spp. by Traditional and PCR Assays in Raw Milk, Maysan, Iraq', سامانه مدیریت نشریات علمی, 77(4), pp. 1453-1457. doi: 10.22092/ari.2022.359086.2369
Dawood Saleem, H, Fawwaz Alfarras, A, Hameed, N. M, Hasan Al-Zubaidi, S, Shnain Ali, M, Hamood, S. A, Hameed, S, Hamad, D. A, Ali Hussein, H, Mohsin Al-Dhalemi, D. Detection of Salmonella spp. by Traditional and PCR Assays in Raw Milk, Maysan, Iraq. سامانه مدیریت نشریات علمی, 1401; 77(4): 1453-1457. doi: 10.22092/ari.2022.359086.2369

Detection of Salmonella spp. by Traditional and PCR Assays in Raw Milk, Maysan, Iraq

Archives of Razi Institute
مقاله 19، دوره 77، شماره 4، مهر و آبان 2022، صفحه 1453-1457    اصل مقاله (536.79 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359086.2369
نویسندگان
H Dawood Saleem1؛ A Fawwaz Alfarras2؛ N. M Hameed3؛ S Hasan Al-Zubaidi4؛ M Shnain Ali5؛ S. A Hamood6؛ S Hameed7؛ D. A Hamad8؛ H Ali Hussein9؛ D Mohsin Al-Dhalemi* 10
1Al-Manara College for Medical Sciences, Maysan, Iraq
2Medical Laboratory Techniques Department, College of Medical Techology, Al-Farahidi University, Baghdad, Iraq
3Anesthesia techniques, Al–Nisour University College, Baghdad, Iraq
4Anesthesia Techniques Department, Al-Mustaqbal University College, Babylon, Iraq
5Department of Dentistry, Al-Zahrawi University College, Karbala, Iraq
6Al-Esraa University College, Baghdad, Iraq
7Medical Device Engineering, Ashur University College, Baghdad, Iraq
8Nursing Department, Hilla University College, Babylon, Iraq
9Scientific Research Center, Al-Ayen University, Thi-Qar, Iraq
10The Islamic University, Najaf, Iraq
چکیده
Salmonella spp are characterized as rod- shaped, motile, gram- negative bacteria which has the ability to infect animals and human. Salmonella spp occasionally causes sickness while in most cases not lead to severe symptoms. Analyzing milk for Salmonella spp. is not routine but traditional culture methods are used to evaluate the health condition of the dairy products. However, the antibody-based and nucleic-acid- based methods are practical for identifying Salmonella spp. Therefore, this research was designed to evaluate the use of traditional culture methods and PCR in detection of the presence of Salmonella spp. in raw milk samples in, Maysan Iraq. A total number of 130 raw milk samples collected from Maysan Iraq. All the samples were analyzed for the presence of Salmonella spp. using traditional culture method and polymerase chain reaction (PCR).  The culture method used in this experiment were done by using pre-enrichment, enrichment, selective plating and biochemical tests. The results of this traditional technique were compared with the results obtained from PCR method. The PCR was performed using a 284bp sequence of the invA gene.  The results showed that 8 (7.07%) of samples were identified as salmonella positive using traditional culture technique but 14 (12.3%) samples were detected as salmonella positive by PCR method. The results of the current research revealed that the traditional culture based methods are generally time costuming and labor intensive but the development of new rapid methods including DNA based methods such as PCR are more sensitive and have dramatically decreased the time necessary for the detection of bacteria.
کلیدواژه‌ها
PCR؛ biochemical tests؛ raw milk؛ Salmonella spp
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مراجع
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