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Extraction and characterization of collagen from the skin and bone of shabout (Arabibarbus grypus Heckel, 1843) | ||
| Iranian Journal of Fisheries Sciences | ||
| دوره 21، شماره 3، مرداد 2022، صفحه 671-687 اصل مقاله (861.56 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2022.126898 | ||
| نویسنده | ||
| M. Göçer* | ||
| Department of Aquaculture and Fisheries Program, Kahta Vocational Training School, Adıyaman University, Adıyaman, Turkey | ||
| چکیده | ||
| Acid soluble collagen (ASC) from the skin (ASC-S) and bone (ASC-B) of shabout (Arabibarbus grypus Heckel, 1843) were isolated and characterized. Both ASC-S and ASC-B from shabout contained glycine as the major amino acid and high amount of proline (Pro), hydroxyproline (Hyp), alanine and glutamic acid. On the basis of dry weight, yields of ASC-S and ASC-B were 6.79 and 2.57%, respectively. Furthermore, fourier transform infrared spectroscopy (FTIR) proved that both collagens were integrated and native. The denaturation temperature of ASC-S and ASC-B were 31.59 and 32.25°C, respectively. Additionally, the results of X-ray diffraction (XRD) proved that the two products retained their helical structures. These collagens had prominent absorptions at 230 nm by UV-Vis spectra. Additionally, the scanning electron microscope (SEM) studies have shown that ACS-S and ASC-B are porous and exhibited fibrous nature. According to the UV–Vis and FTIR results, extracted collagens were characterized as Type I collagen based on their amino acid profile. In the current study, the total amount of amino acid (Pro+Hyp) was 19.28% and 19.55% for ASC-S and ASC-B, respectively. The results of the current study suggested that the collagen isolated from shabout can potentially be an alternative source for use in the food, pharmaceutical and biomedical industries. | ||
| کلیدواژهها | ||
| Shabout (Arabibarbus grypus)؛ Characterization؛ FTIR؛ SEM skin؛ bone؛ Type I collagen | ||
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