Fekri, K, Mortezaie, M. Sh, Maleki-Dizaji, N, Fathiazad, F, Hamedeyazdan, S. (1401). Marrubium persicum İmproved the Biological Parameters Associated with Angiogenesis and İnflammation in Mice. سامانه مدیریت نشریات علمی, 78(1), 233-239. doi: 10.22092/ari.2022.358579.2253
K Fekri; M. Sh Mortezaie; N Maleki-Dizaji; F Fathiazad; S Hamedeyazdan. "Marrubium persicum İmproved the Biological Parameters Associated with Angiogenesis and İnflammation in Mice". سامانه مدیریت نشریات علمی, 78, 1, 1401, 233-239. doi: 10.22092/ari.2022.358579.2253
Fekri, K, Mortezaie, M. Sh, Maleki-Dizaji, N, Fathiazad, F, Hamedeyazdan, S. (1401). 'Marrubium persicum İmproved the Biological Parameters Associated with Angiogenesis and İnflammation in Mice', سامانه مدیریت نشریات علمی, 78(1), pp. 233-239. doi: 10.22092/ari.2022.358579.2253
Fekri, K, Mortezaie, M. Sh, Maleki-Dizaji, N, Fathiazad, F, Hamedeyazdan, S. Marrubium persicum İmproved the Biological Parameters Associated with Angiogenesis and İnflammation in Mice. سامانه مدیریت نشریات علمی, 1401; 78(1): 233-239. doi: 10.22092/ari.2022.358579.2253
Marrubium persicum İmproved the Biological Parameters Associated with Angiogenesis and İnflammation in Mice
1Department of Paramedicine, Amol School of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran
2Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
5Research Center for Integrative Medicine in Aging, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
The genus Marrubium, belonging to the family Lamiaceae, is highly praised in herbal medicine of different countries for having renowned healing properties. Herein, the anti-inflammatory and anti-angiogenesis potential of Marrubium persicum methanol extract was evaluated in a mouse air pouch model of inflammation. Aerial parts of M. persicum were solvent extracted using the Soxhlet apparatus. Subsequently, air injections were performed (for 3 days) into the mice's backs to bring about an air pouch, while carrageenan was used to induce inflammation. The mice were divided into four groups, including; negative control (normal saline into the pouch), control (carrageenan), treatment and positive control (dexamethasone). The inflammatory markers were analyzed 48h after injecting carrageenan, and a haemoglobin assay kit assessed the quantification of angiogenesis in granulation tissue. M. persicum methanol extract at doses of 3.5, 5, 7.5 and 10 mg/kg represented significant decreases in inflammatory parameters. Compared to the control group, the optimum dose (3.5 mg/kg) lessened the myeloperoxidase (MPO) and angiogenesis activity, as well as haemoglobin levels. In sum, the methanol extract of M. persicum exhibited anti-inflammatory effects against carrageenan-induced inflammation, which could be related to its antioxidant and inhibitory effects on neutrophils’ infiltration.
گیاه ماروبیوم پرسیکوم پارامترهای بیولوژیک مرتبط با التهاب و رگ زایی را در موش های سوری بهبود بخشید
چکیده [English]
جنس ماروبیوم متعلق به خانواده لامیاسه در طب گیاهی کشورهای مختلف به دلیل داشتن خواص درمانی معروف بسیار مورد توجه قرار گرفته است. در اینجا، پتانسیل ضد التهابی و ضد رگزایی عصاره متانولی ماروبیوم پرسیکوم در مدل التهاب کیسه هوای موش بررسی شد. قسمت های هوایی ماروبیوم پرسیکوم با استفاده از دستگاه سوکسله با حلال استخراج شد. پس از آن، تزریق هوا (به مدت 3 روز) به پشت موش ها انجام شد تا یک کیسه هوا ایجاد شود در حالی که از کاراگینان برای القای التهاب استفاده شد. موش ها به چهار گروه تقسیم شدند. کنترل منفی (سالین نرمال داخل کیسه)، کنترل (کاراگینان)، درمان و کنترل مثبت (دگزامتازون). نشانگرهای التهابی 48 ساعت پس از تزریق کاراگینان مورد تجزیه و تحلیل قرار گرفتند و کمیت رگزایی در بافت گرانولاسیون با استفاده از کیت سنجش هموگلوبین ارزیابی شد. عصاره متانولی ماروبیوم پرسیکوم در دوزهای 3.5، 5، 7.5 و 10 میلی گرم بر کیلوگرم کاهش قابل توجهی در پارامترهای التهابی نشان داد. در مقایسه با گروه کنترل، دوز بهینه (3.5 میلی‌گرم بر کیلوگرم) فعالیت میلوپراکسیداز و رگزایی و همچنین سطوح هموگلوبین را به‌طور معنی‌داری کاهش داد. در مجموع، عصاره متانولی ماروبیوم پرسیکوم اثرات ضد التهابی در برابر التهاب ناشی از کاراگینان از خود نشان داد که می‌تواند به اثرات آنتی اکسیدانی آن و همچنین اثرات بازدارنده بر نفوذ نوتروفیل‌ها مربوط باشد.
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