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آمار
| تعداد نشریات | 280 |
| تعداد نشریات سازمان | 79 |
| تعداد شماره‌ها | 2,348 |
| تعداد مقالات | 27,179 |
| تعداد مشاهده مقاله | 14,960,606 |
| تعداد دریافت فایل اصل مقاله | 11,950,805 |
Metformin and Bee Venom: A Comparative Detection of Histological Alteration of the Pancreas and Systemic Inflammatory Markers in Diabetic Mice | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 خرداد 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.358619.2269 | ||
| نویسندگان | ||
Rana A.H. Al-Sarray  1؛ Sattar J. J. Al-Shaeli2
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| 1Department of Biological Science, College of Education for Pure Sciences, Wasit University, Wasit, Iraq | ||
| 2Department of Medical Basic Sciences, College of Dentistry, Wasit University, Wasit, Iraq | ||
| چکیده | ||
| Metformin is widely used as the approved prescribed medication that used for management global health issue concerned type 2 diabetes mellitus (T2DM). It is crucial to use natural bioactive compounds as alternative therapy to manage several metabolic diseases. Therefore, due to recent limited studies that detected the role of bee venom (BV) to improve diabetic condition in Iraq, the current study was designed to identify the potential therapeutic role of BV and metformin in diabetic mice. 20 male mice (Balb/c) aged about 60 days with an average weight of 26.55 ± 2.70 g were randomly divided into four groups (n=5). The animals were placed in plastic cages for acclimatization for one week access to food and water ad libitum. Overnight fasting was applied to 15 mice which then injected with 95 mg/kg body weight of prepared alloxan. The mice were supplemented with glucose fluid for 3 days. At day 4, the blood was collected from the tail to measure the level of circulating glucose. When the level of blood glucose exceeding 200 mg/dl the animals considered diabetic. After induction of diabetes the animals were divided as following: Control group: including five mice that not subjected to diabetes induction, the animals in this group did not received any medications. Diabetic group: including five mice that were confirmed with diabetes without receiving any treatments. Metformin group: including five diabetic mice exposed to oral single dose of 150 mg/kg metformin for 30 days. Bee Venom group: including five diabetic mice exposed to intraperitoneal single dose of 1 mg/kg Bee Venom for 30 days. After 30 days of treatment, blood was drained and serum was obtained to detect the levels of glucose, insulin, TNFα, IL6, and IL10 by using precise enzyme-linked immunosorbent assay (ELIZA) kits. Also, the pancreas was collected from all mice for histopathological investigation. The result displayed significant elevated of glucose concentration in diabetic mice, while metformin and BV significantly reversed this increases. Significant declined in the concentration of insulin was seen in diabetic mice, whereas metformin and BV significantly enhanced this reduction in the concentration of insulin. | ||
| کلیدواژه‌ها | ||
| Diabetes mellitus, TNF&alpha؛ , IL-6, IL-10 | ||
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آمار تعداد مشاهده مقاله: 4 |
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