Comparison between Polymerase Chain Reaction and blood culture for diagnosis of neonatal sepsis

Hayder  Hamad, Mohammed; Eidan Hadi, Mazin; K. Ajam, Ismael

doi:10.22092/ari.2022.358608.2259

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	Comparison between Polymerase Chain Reaction and blood culture for diagnosis of neonatal sepsis
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 خرداد 1401
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358608.2259
نویسندگان
Mohammed Hayder Hamad ¹؛ Mazin Eidan Hadi²؛ Ismael K. Ajam³
¹1,2Al Mustaqbal University college, Babylon , Iraq
²Al Mustaqbal University college, Babylon , Iraq
³Islamic University / Babylon , Iraq
چکیده
Neonatal sepsis can be defined as any systemic bacterial infection confirmed by a positive blood culture in the first month of life. This study was designed to evaluate the polymerase chain reaction as the diagnostic approach to identify the neonatal sepsis instead of blood culture. In this study, 85 blood specimens were collected from 85 patients with suspected septicemia, age ranged between 1to 28 days from both sexes (53 males and 32 females) during the period from November 2014 to March 2015. From each neonate, a minimum of 1–3 ml of blood was collected by standard sterile procedures 2 ml for blood culture while 1 ml used for DNA extraction. A minimum of 2 ml of blood is taken through venipuncture and injected into two or more "blood bottles" with specific media for aerobic and anaerobic organisms. The blood is collected using aseptic technique. The recorded data showed that the bacterial culture was positive in 7.06% patients versus 92.9% patients revealed a negative bacterial culture. The most common types of bacteria isolated were three isolates of Klebsiella spp. (50.0%), followed by one isolate of Staphylococcus aureus (16.67%), one E. coli (16.67%) isolate, and one Enterobacter spp.(16.67%) isolate. Finally, molecular detection for bacterial sepsis was done by using specific primers (16 sRNA, rpoB and its). It was found that 16 sRNA genes were present in 20% of samples and rpoBgene present in (18.8%). While its gene used for detection of fungi revealed negative results in all samples.
کلیدواژه‌ها
Neonatal sepsis؛ 16 sRNA؛ PCR؛ Septicemia

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Comparison between Polymerase Chain Reaction and blood culture for diagnosis of neonatal sepsis