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Al-Mudallal, N. H. A. L. (1401). The Expression of MMP1 and MMP7 in Mice Liver after Exposure to Aflatoxin B1 Using Immunohistochemistry Technique. سامانه مدیریت نشریات علمی, 78(1), 63-72. doi: 10.22092/ari.2022.358774.2306
N. H. A. L Al-Mudallal. "The Expression of MMP1 and MMP7 in Mice Liver after Exposure to Aflatoxin B1 Using Immunohistochemistry Technique". سامانه مدیریت نشریات علمی, 78, 1, 1401, 63-72. doi: 10.22092/ari.2022.358774.2306
Al-Mudallal, N. H. A. L. (1401). 'The Expression of MMP1 and MMP7 in Mice Liver after Exposure to Aflatoxin B1 Using Immunohistochemistry Technique', سامانه مدیریت نشریات علمی, 78(1), pp. 63-72. doi: 10.22092/ari.2022.358774.2306
Al-Mudallal, N. H. A. L. The Expression of MMP1 and MMP7 in Mice Liver after Exposure to Aflatoxin B1 Using Immunohistochemistry Technique. سامانه مدیریت نشریات علمی, 1401; 78(1): 63-72. doi: 10.22092/ari.2022.358774.2306

The Expression of MMP1 and MMP7 in Mice Liver after Exposure to Aflatoxin B1 Using Immunohistochemistry Technique

Archives of Razi Institute
مقاله 10، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 63-72    اصل مقاله (738.44 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358774.2306
نویسنده
N. H. A. L Al-Mudallal*
Department of Medical Microbiology, College of Medicine, Al-Iraqi University, Baghdad, Iraq
چکیده
Mycotoxin is a class of poisonous secondary metabolites generated by filamentous fungi and found in agricultural commodities worldwide. Therefore, the current study aimed to investigate how aflatoxin B1 affected hepatic cellular architecture and Matrix metalloproteinase expression in particular (MMP1 and MMP7) in the livers of experimental mice (IHC). A total of sixteen mice (four groups) were studied after being given pure aflatoxin B1 (9mg/kg B.W., 6mg/kg B.W., and 3mg/kg B.W.) (produced from Aspergillus flavus) or a control group (not treated). MMP1 and MMP7 expressions were also measured using the MMP1 and MMP7 expression assays (IHC). The degree of liver damage is related to the AFB1 concentration and the duration of exposure. IHC reveals a considerable rise in MMP1 and MMP7 expression in the livers of mice given a maximum concentration of 90% ((9 mg/B.W.) pure AFB1), which approached the toxin's effect toxic dosage. MMP1 and MMP7 expression were also increased by AFB1 at dosages of 60 and 30% (6mg/BW and 3mg/B.W., respectively), although not to the same extent as 90%. MMP1 was significantly more expressed than MMP7 compared to control, and AFB1 at 90, 60, and 30% concentrations caused changes in hepatic cellular architecture, organization, and liver tissue damage and dramatically increased MMP1 and MMP7 production in hepatic tissue following treatment. Increased levels of pure aflatoxin B1 will harm liver tissue and MMP1 and MMP7 expression. MMP1 was more substantially expressed than MMP7.
کلیدواژه‌ها
Matrix metalloproteinases MMP1 and MMP7؛ Aflatoxin B1؛ Immunohistochemistry technique
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مراجع
  1. Liew W-P-P, Mohd-Redzwan S. Mycotoxin: Its impact on gut health and microbiota. Front Cell Infect. 2018;8:60.
  2. Omotayo OP, Omotayo AO, Mwanza M, Babalola OO. Prevalence of mycotoxins and their consequences on human health. Toxicol Res. 2019;35(1):1-7.
  3. WHO. Hazardous chemicals in human and environmental health. Geneva, Switzerland: World Health Organization. 2002.
  4. Dai Y, Huang K, Zhang B, Zhu L, Xu W. Aflatoxin B1-induced epigenetic alterations: An overview. Food Chem Toxicol. 2017;109:683-9.
  5. Muhammad I, Sun X, Wang H, Li W, Wang X, Cheng P, et al. Curcumin successfully inhibited the computationally identified CYP2A6 enzyme-mediated bioactivation of aflatoxin B1 in arbor acres broiler. Front Pharmacol. 2017;8:143.
  6. Naim A, Pan Q, Baig MS. Matrix metalloproteinases (MMPs) in liver diseases. J Clin Exp Hepatol. 2017;7(4):367-72.
  7. Scheau C, Badarau IA, Costache R, Caruntu C, Mihai GL, Didilescu AC, et al. The role of matrix metalloproteinases in the epithelial-mesenchymal transition of hepatocellular carcinoma. Anal Cell Pathol. 2019;2019.
  8. Yamahana H, Terashima M, Takatsuka R, Asada C, Suzuki T, Uto Y, et al. TGF-β1 facilitates MT1-MMP-mediated proMMP-9 activation and invasion in oral squamous cell carcinoma cells. Biochem Biophys Rep. 2021;27:101072.
  9. Niland S, Riscanevo AX, Eble JA. Matrix metalloproteinases shape the tumor microenvironment in cancer progression. Int J Mol Sci. 2021;23(1):146.
  10. Cabral-Pacheco GA, Garza-Veloz I, Castruita-De la Rosa C, Ramirez-Acuna JM, Perez-Romero BA, Guerrero-Rodriguez JF, et al. The roles of matrix metalloproteinases and their inhibitors in human diseases. Int J Mol Sci. 2020;21(24):9739.
  11. Lai Y-L, Gong C-L, Fu C-K, Yueh T-C, Tsai C-W, Chang W-S, et al. The contribution of matrix metalloproteinase-1 genotypes to hepatocellular carcinoma susceptibility in Taiwan. Cancer Genom Proteom. 2017;14(2):119-25.
  12. Djuric T, Zivkovic M. Overview of MMP biology and gene associations in human diseases. Role Matrix Met Hum Body Pathol. 2017;1(3).
  13. Al-Mudallal NH, Almashta HIA, Rashid SA, Nakeeb A. Study the Expression of Tumor Growth Factor β (TGF β) in Mice Organs after Exposure to Aflatoxin B1 using Immunohistochemistry Technique. Extraction. 2009;11:12.
  14. Park D, Troxell T. Mycotoxins and food safety. Adv Exp Med Biol. 2002;504:173-9.
  15. Bancroft JD, Gamble M. Theory and practice of histological techniques: Elsevier health sciences; 2008.
  16. Benson. Microbial Applications Laboratory Manual in General Microbiology: The McGraw-Hill Companies; 2001.
  17. Wang QM, Lv L, Tang Y, Zhang L, Wang LF. MMP‑1 is overexpressed in triple‑negative breast cancer tissues and the knockdown of MMP‑1 expression inhibits tumor cell malignant behaviors in vitro. Oncol Lett. 2019;17(2):1732-40.
  18. Yuan S, Lin L-s, Gan R-H, Huang L, Wu X-t, Zhao Y, et al. Elevated matrix metalloproteinase 7 expression promotes the proliferation, motility and metastasis of tongue squamous cell carcinoma. BMC Cancer. 2020;20(1):1-12.
  19. Ito K, Kitajima Y, Kai K, Matsufuji S, Yamada K, Egawa N, et al. Matrix metalloproteinase‑1 expression is regulated by HIF‑1‑dependent and epigenetic mechanisms and serves a tumor‑suppressive role in gastric cancer progression. Int J Cancer. 2021;59(6):1-15.
  20. Celentano DD, Platz E, Mehta SH. The centennial of the Department of Epidemiology at Johns Hopkins Bloomberg School of Public Health: a century of epidemiologic discovery and education. Am J Epidemiol. 2019;188(12):2043-8.
  21. Marchese S, Polo A, Ariano A, Velotto S, Costantini S, Severino L. Aflatoxin B1 and M1: Biological properties and their involvement in cancer development. Toxins. 2018;10(6):214.
  22. Ishikawa AT, Hirooka EY, Alvares e Silva PL, Bracarense APFRL, Flaiban KKMdC, Akagi CY, et al. Impact of a single oral acute dose of aflatoxin B1 on liver function/cytokines and the lymphoproliferative response in C57Bl/6 mice. Toxins. 2017;9(11):374.
  23. Guengerich FP, Johnson WW, Shimada T, Ueng Y-F, Yamazaki H, Langouët S. Activation and detoxication of aflatoxin B1. Mutat Res Fundam Mol Mech. 1998;402(1-2):121-8.
  24. Benkerroum N. Chronic and acute toxicities of aflatoxins: Mechanisms of action. Int J Environ Res Public Health. 2020;17(2):423.
  25. Xu Q, Shi W, Lv P, Meng W, Mao G, Gong C, et al. Critical role of caveolin-1 in aflatoxin B1-induced hepatotoxicity via the regulation of oxidation and autophagy. Cell Death Dis. 2020;11(1):1-16.
  26. de Freitas Souza C, Baldissera MD, Baldisserotto B, Petrolli TG, da Glória EM, Zanette RA, et al. Dietary vegetable choline improves hepatic health of Nile tilapia (Oreochromis niloticus) fed aflatoxin-contaminated diet. Comp Biochem Physiol C Toxicol Pharmacol. 2020;227:108614.
  27. Singh C, Prakash C, Mishra P, Tiwari KN, Mishra SK, More RS, et al. Hepatoprotective efficacy of Premna integrifolia L. leaves against aflatoxin B1-induced toxicity in mice. Toxicon. 2019;166:88-100.
  28. Xu F, Li Y, Cao Z, Zhang J, Huang W. AFB1-induced mice liver injury involves mitochondrial dysfunction mediated by mitochondrial biogenesis inhibition. Ecotoxicol Environ Saf. 2021;216:112213.
  1. Chen F, Feng L, Zheng Y-l, Lu J, Fan S-h, Shan Q, et al. 2, 2′, 4, 4′-tetrabromodiphenyl ether (BDE-47) induces mitochondrial dysfunction and related liver injury via eliciting miR-34a-5p-mediated mitophagy impairment. Environ Pollut. 2020;258:113693.
  2. Liu X, Taylor SA, Gromer KD, Zhang D, Hubchak SC, LeCuyer BE, et al. Mechanisms of liver injury in high fat sugar diet fed mice that lack hepatocyte X-box binding protein 1. Plos One. 2022;17(1):e0261789.
  3. Eiró N, González LO, Cid S, Andicoechea A, Vizoso FJ. Matrix metalloproteases expression in different histological types of colorectal polyps. Rev Esp Enferm Dig. 2017;109(6):414-20.
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